Bz-rA Phosphoramidite
Based on 1 Customer Validation
Bz-rA Phosphoramidite (DMT-2'O-TBDMS-rA(bz) Phosphoramidite) a phosphoramidite containing 5' DMT and the nucleoside adenosine (A) with Benzoyl (Bz) base protection, for use in oligonucleotide synthesis.
For research use only. We do not sell to patients.
- Purity: 99.03%
- CAS No.: 104992-55-4
- Formula: C53H66N7O8PSi
- Molecular Weight:988.19
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Bz-rA Phosphoramidite is a 2'-O-protected ribonucleoside phosphoramidite, which can be used in the synthesis of 2'-O-guanidinopropyl-modified nucleoside phosphoramidites.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 104992-55-4
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Appearance Solid
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Molecular Weight 988.19
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Formula C53H66N7O8PSi
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(C2=CC=CC=C2)(C3=CC=C(OC)C=C3)OC[C@@H]4[C@@H](OP(N(C(C)C)C(C)C)OCCC#N)[C@@H](O[Si](C)(C)C(C)(C)C)[C@H](N5C6=NC=NC(NC(C7=CC=CC=C7)=O)=C6N=C5)O4
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Synonyms
DMT-2'O-TBDMS-rA(bz) Phosphoramidite
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (101.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (2.53 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0120 mL | 5.0598 mL | 10.1195 mL | 25.2988 mL |
| 5 mM | 0.2024 mL | 1.0120 mL | 2.0239 mL | 5.0598 mL | |
| 10 mM | 0.1012 mL | 0.5060 mL | 1.0120 mL | 2.5299 mL | |
| 15 mM | 0.0675 mL | 0.3373 mL | 0.6746 mL | 1.6866 mL | |
| 20 mM | 0.0506 mL | 0.2530 mL | 0.5060 mL | 1.2649 mL | |
| 25 mM | 0.0405 mL | 0.2024 mL | 0.4048 mL | 1.0120 mL | |
| 30 mM | 0.0337 mL | 0.1687 mL | 0.3373 mL | 0.8433 mL | |
| 40 mM | 0.0253 mL | 0.1265 mL | 0.2530 mL | 0.6325 mL | |
| 50 mM | 0.0202 mL | 0.1012 mL | 0.2024 mL | 0.5060 mL | |
| 60 mM | 0.0169 mL | 0.0843 mL | 0.1687 mL | 0.4216 mL | |
| 80 mM | 0.0126 mL | 0.0632 mL | 0.1265 mL | 0.3162 mL | |
| 100 mM | 0.0101 mL | 0.0506 mL | 0.1012 mL | 0.2530 mL |
Bz-rA Phosphoramidite Related Classifications
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