Ac-rC Phosphoramidite
Based on 1 Customer Validation
Ac-rC Phosphoramidite is a cytosine phosphoramidite used in the chemical synthesis of RNA oligonucleotides. Ac-rC Phosphoramidite enables the incorporation of cytidine nucleotides into synthetic RNA strands, and its unique protecting groups ensure the structural integrity of the molecule throughout complex synthetic processes.
For research use only. We do not sell to patients.
- Purity: 98.87%
- CAS No.: 121058-88-6
- Formula: C47H64N5O9PSi
- Molecular Weight:902.10
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
More
Biological Activity
Ac-rC Phosphoramidite enables the incorporation of cytidine nucleotides into synthetic RNA strands, and its unique protecting groups ensure the structural integrity of the molecule throughout complex synthetic processes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 121058-88-6
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Appearance Solid
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Molecular Weight 902.10
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Formula C47H64N5O9PSi
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(C2=CC=CC=C2)(C3=CC=C(OC)C=C3)OC[C@@H]4[C@@H](OP(N(C(C)C)C(C)C)OCCC#N)[C@@H](O[Si](C)(C)C(C)(C)C)[C@H](N5C(N=C(NC(C)=O)C=C5)=O)O4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (110.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (2.77 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1085 mL | 5.5426 mL | 11.0852 mL | 27.7131 mL |
| 5 mM | 0.2217 mL | 1.1085 mL | 2.2170 mL | 5.5426 mL | |
| 10 mM | 0.1109 mL | 0.5543 mL | 1.1085 mL | 2.7713 mL | |
| 15 mM | 0.0739 mL | 0.3695 mL | 0.7390 mL | 1.8475 mL | |
| 20 mM | 0.0554 mL | 0.2771 mL | 0.5543 mL | 1.3857 mL | |
| 25 mM | 0.0443 mL | 0.2217 mL | 0.4434 mL | 1.1085 mL | |
| 30 mM | 0.0370 mL | 0.1848 mL | 0.3695 mL | 0.9238 mL | |
| 40 mM | 0.0277 mL | 0.1386 mL | 0.2771 mL | 0.6928 mL | |
| 50 mM | 0.0222 mL | 0.1109 mL | 0.2217 mL | 0.5543 mL | |
| 60 mM | 0.0185 mL | 0.0924 mL | 0.1848 mL | 0.4619 mL | |
| 80 mM | 0.0139 mL | 0.0693 mL | 0.1386 mL | 0.3464 mL | |
| 100 mM | 0.0111 mL | 0.0554 mL | 0.1109 mL | 0.2771 mL |
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