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Cat. No.: HY-128536

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KMG-104AM

KMG-104AM is a Mg²⁺fluorescent probe. KMG-104AM is a membrane-permeable ester-modified derivative of KMG-104, which serves as a reporter and imaging agent. KMG-104AM can track the increase in intracellular free magnesium ion concentration induced by mitochondrial uncoupling. KMG-104AM enables visualization of the three-dimensional distribution of intracellular magnesium ion concentration. KMG-104AM is applicable to research related to the functions of intracellular magnesium ions.

For research use only. We do not sell to patients.

CAS No.: 2436544-28-2
Formula: C₂₈H₁₇F₂NO₉
Molecular Weight:549.43

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

KMG-104AM Chemical Structure

CAS No.: 2436544-28-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Purity & Documentation
References
Calculators
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Classifications
Bioz

Biological Activity

Description

In Vitro

KMG-104AM (10 μM; 30 min, 15 min) enables detection of dynamic intracellular Mg²⁺ concentration changes in differentiated PC12 cells, measuring a resting Mg²⁺ concentration of 0.90 mM and capturing FCCP-induced Mg²⁺ elevation and subsequent recovery^[1].
KMG-104AM (10 μM; 30 min, 15 min) enables high-resolution 3D imaging of intracellular Mg²⁺ distributions in differentiated PC12 cells, revealing concentrated Mg²⁺ or probe localization in nucleolia-like regions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

514

Excitation (Ex)

495

Chemical Information

CAS No. 2436544-28-2
Molecular Weight 549.43
Formula C₂₈H₁₇F₂NO₉
SMILES

O=C1C(C(OCOC(C)=O)=O)=CC(C2=C3C=C(F)C(C=C3OC4=C2C=C(F)C(OC(C)=O)=C4)=O)=C5C=CC=CN15
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Komatsu H, et al. Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging. J Am Chem Soc. 2004;126(50):16353-16360. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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