2'-Hydroxy-3,4-dimethoxychalcone
Based on 1 Customer Validation
2'-Hydroxy-3,4-dimethoxychalcone is a chalcone derivative and lipoxygenase inhibitor with an IC50 of 67.5 μM. 2'-Hydroxy-3,4-dimethoxychalcone acts as a radical scavenger that scavenges DPPH stable free radical, superoxide anion radical, and hydroxyl radical. 2'-Hydroxy-3,4-dimethoxychalcone scavenges hydrogen peroxide and inhibits linoleic acid lipid peroxidation. 2'-Hydroxy-3,4-dimethoxychalcone can be used for research on oxidative damage and inflammatory diseases.
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- Pureté : 98.01%
- CAS No.: 19152-36-4
- Formule: C17H16O4
- Masse moléculaire:284.31
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | IC50 |
95 μM
Compound: 24
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Growth inhibition of MCF7 cells by sulforhodamine assay
Growth inhibition of MCF7 cells by sulforhodamine assay
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[PMID: 17383189] |
| MDCK-II | IC50 |
4.97 μM
Compound: 5
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Inhibition of human BCRP expressed in MDCK2 cells assessed as Hoechst 33342 accumulation preincubated for 30 mins by fluorimetry
Inhibition of human BCRP expressed in MDCK2 cells assessed as Hoechst 33342 accumulation preincubated for 30 mins by fluorimetry
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[PMID: 22112540] |
| Neutrophil | IC50 |
15 μM
Compound: 13
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Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 30 mM glucose by luminol-amplified chemiluminescence method
Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 30 mM glucose by luminol-amplified chemiluminescence method
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[PMID: 33006891] |
| Neutrophil | IC50 |
6.4 μM
Compound: 13
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Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 5.5 mM glucose by luminol-amplified chemiluminescence method
Inhibition of PMA-induce ROS/RNS generation in human neutrophils measured up to 30 mins in presence of 5.5 mM glucose by luminol-amplified chemiluminescence method
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[PMID: 33006891] |
| TK-10 | IC50 |
90 μM
Compound: 24
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Growth inhibition of TK10 cells by sulforhodamine assay
Growth inhibition of TK10 cells by sulforhodamine assay
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[PMID: 17383189] |
In Vitro
2'-Hydroxy-3,4-dimethoxychalcone (Compound 12) (50-100 μM; 20-60 min) exhibits time- and concentration-dependent DPPH radical scavenging activity, reaching 28.5% scavenging at 100 μM after 60 min incubation[1].
2'-Hydroxy-3,4-dimethoxychalcone (10 μM) completely scavenges superoxide anion radicals[1].
2'-Hydroxy-3,4-dimethoxychalcone (100 μM) scavenges hydroxyl radicals with 84.1% inhibition[1].
2'-Hydroxy-3,4-dimethoxychalcone scavenges hydrogen peroxide with an IC50 of 0.228 mM[1].
2'-Hydroxy-3,4-dimethoxychalcone (10 μM) inhibits AAPH (HY-Y0525)-induced linoleic acid lipid peroxidation with 64.6% activity[1].
2'-Hydroxy-3,4-dimethoxychalcone (67.5 μM) inhibits soybean lipoxygenase with an IC50 of 67.5 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 19152-36-4
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Appearance Solid
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Masse moléculaire 284.31
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Formule C17H16O4
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Color Yellow to orange
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SMILES
O=C(C1=C(O)C=CC=C1)C=CC2=CC=C(C(OC)=C2)OC
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Structure Classification
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 25 mg/mL (87.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.79 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5173 mL | 17.5864 mL | 35.1729 mL | 87.9322 mL |
| 5 mM | 0.7035 mL | 3.5173 mL | 7.0346 mL | 17.5864 mL | |
| 10 mM | 0.3517 mL | 1.7586 mL | 3.5173 mL | 8.7932 mL | |
| 15 mM | 0.2345 mL | 1.1724 mL | 2.3449 mL | 5.8621 mL | |
| 20 mM | 0.1759 mL | 0.8793 mL | 1.7586 mL | 4.3966 mL | |
| 25 mM | 0.1407 mL | 0.7035 mL | 1.4069 mL | 3.5173 mL | |
| 30 mM | 0.1172 mL | 0.5862 mL | 1.1724 mL | 2.9311 mL | |
| 40 mM | 0.0879 mL | 0.4397 mL | 0.8793 mL | 2.1983 mL | |
| 50 mM | 0.0703 mL | 0.3517 mL | 0.7035 mL | 1.7586 mL | |
| 60 mM | 0.0586 mL | 0.2931 mL | 0.5862 mL | 1.4655 mL | |
| 80 mM | 0.0440 mL | 0.2198 mL | 0.4397 mL | 1.0992 mL |
2'-Hydroxy-3,4-dimethoxychalcone Related Classifications
HY-W110154 Related Classifications
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Keywords
- 2'-Hydroxy-3,4-dimethoxychalcone
- 19152-36-4
- Lipoxygenase
- Reactive Oxygen Species (ROS)
- superoxide anion radical
- 13-hydroperoxylinoleic acid
- hydrogen peroxide
- DPPH stable free radical
- hydroxyl radical
- soybean lipoxygenase
- Sophora interrupta Bedd.
- root tissue
- sodium linoleate
- AAPH-induced linoleic acid lipid peroxidation
- Inhibitor
- inhibitor
- inhibit