PRO-HD3
PRO-HD3 is a HDAC6 PROTAC degrader. PRO-HD3 exerts antiproliferative activity in leukemia cells. PRO-HD3 can be used for leukemia research.
(Pink: HDAC6 ligand (HY-175627); Blue: Cereblon ligand (HY-41547); Black: linker (HY-W015659)).
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- CAS No.: 3126555-94-7
- Formule: C40H43N5O5S
- Masse moléculaire:705.86
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
(Pink: HDAC6 ligand (HY-175627); Blue: Cereblon ligand (HY-41547); Black: linker (HY-W015659)).
In Vitro
PRO-HD3 (500 nM-5 μM; 24 h) nearly completely degrades HDAC6 in A549 cells at a concentration of 5 μM after 24 h of incubation[1].
PRO-HD3 (48 h) inhibits Jurkat cell proliferation with an IC50 of 3.80 μM after 48 h of incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:adenocarcinomic human alveolar basal epithelial A549 cells
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Concentration:500 nM, 1 μM, 5 μM
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Incubation Time:24 h
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Result:Nearly completely depleted HDAC6 protein at 5 μM.
Partially degraded HDAC6 protein at lower concentrations.
Chemical Information
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CAS No. 3126555-94-7
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Masse moléculaire 705.86
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Formule C40H43N5O5S
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SMILES
S=C1N(CC2=CC=C(C3=CC=C(CNCCCCCCCCNC4=CC=CC(C(N5C6CCC(NC6=O)=O)=O)=C4C5=O)C=C3)C=C2)C=CC=C1O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PRO-HD3 Related Classifications
HY-155398 Related Classifications
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