Subtilisin
Based on 1 Customer Validation
Subtilisin (EC 3.4.21.14) is a bacterial serine protease. Subtilisin induces Apoptosis. Subtilisin stimulates the expression of pro-allergic cytokines (IL-1α, IL-33). Subtilisin induces prototypic allergic lung inflammation. Subtilisin exhibits anticancer activity against breast and colon cancer. Subtilisin shows antifouling activity. Subtilisin can be used as a detergent additive.
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- CAS No.: 9014-01-1
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Product Information
It is a proteolytic enzyme made by deep fermentation, extraction and purification of Bacillus subtilis selected by protoplast mutagenesis method. It can hydrolyze the peptide chain of protein molecules to generate polypeptides or amino acids. It can also catalyze the synthesis of polypeptides in organic solvents. This product contains stabilizers.
Effective temperature range: 20-60°C, the optimum temperature is between 35-45°C.
Effective pH range: 6.0-11.0, the optimum pH range is between 9.5-10.5.
Activators: Ca2+, Mg2+, Mn2+, Zn2+, Fe2+.
Inhibitors: Reagents acting on serine such as sulfonyl halides.
Instructions
Soluble in 100 mg/mL ddH2O, avoid repeated freezing and thawing, or dissolve according to specific experimental references.
Subtilisin (3-5 μg/mL; 24 h) shows cytotoxic effects in MCF-7 and HT-29 cancer cells, with MCF-7 cells showing apoptotic cell characteristics and HT-29 cells showing necrotic cell characteristics[1].
Subtilisin shows antifouling activity[2].
Subtilisin (1.0-10 μg/mL; 3-6 h) stimulates the expression of pro-allergic cytokines IL-1α, IL-33, TSLP, and the growth factor amphiregulin in H292 cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7
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Concentration:0 μg/mL, 3 μg/mL, 5 μg/mL
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Incubation Time:24 h
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Result:Increased apoptotic rate.
Showed no changes in the levels of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female C57BL/6 mice (8-12 weeks old), allergic airway disease model[4]
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Dosage:1 μg
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Administration:Subcutaneous (s.c.) or intranasal (i.n.). Sensitization: s.c. or i.n. injection with 1 μg of subtilisin on days 0 and 7. Challenge: i.n. administration with 1 μg of subtilisin on days 14 and 21. whole experimental cycle of 22 days
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Result:Induced high levels of serum IgE and specific IgG1.
Led to intense airway eosinophilic inflammation, as shown by total and differential cell counts in bronchoalveolar lavage (BAL).
Induced pronounced airway inflammation and mucus formation.
Promoted increased bronchoconstriction to inhaled methacholine and induced a late-phase response.
Induced allergic responses dependent on its protease activity, protease-activated receptor (PAR)-2, IL-33 receptor ST2, and MyD88 signaling.
3.4.21.62
≥150 U/mg soild
One unit is defined as the amount of enzyme that can hydrolyze casein to produce 1 μg of tyrosine per min at pH 10 and 40°C
Chemical Information
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CAS No. 9014-01-1
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Appearance Solid
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Color Light yellow to light brown
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SMILES
[Subtilisin]
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Synonyms
EC 3.4.21.14
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvant et solubilité
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Pureté et documentation
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Fiche technique (272 KB)
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SDS (925 KB)
- English - EN (925 KB)
- Français - FR (925 KB)
- Deutsch - DE (925 KB)
- Norwegian - NO (925 KB)
- Español - ES (925 KB)
- Swedish - SV (925 KB)
- Italian - IT (925 KB)
- Korean - KR (925 KB)
- Portuguese - PT (925 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Singh N, et al. Subtilisin from Bacillus amyloliquefaciens induces apoptosis in breast cancer cells through ubiquitin-proteasome-mediated tubulin degradation. Int J Biol Macromol. 2022 Nov 1;220:852-865. [Content Brief]
[2]. Leroy C, et al. Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers. J Appl Microbiol. 2008 Sep;105(3):791-9. [Content Brief]
[3]. Alexander PA, et al. Stabilizing mutations and calcium-dependent stability of subtilisin. Biochemistry. 2001 Sep 4;40(35):10640-4. [Content Brief]
[4]. Florsheim E, et al. Integrated innate mechanisms involved in airway allergic inflammation to the serine protease subtilisin. J Immunol. 2015 May 15;194(10):4621-30. [Content Brief]
[5]. Azrin NAM, et al. Versatility of subtilisin: A review on structure, characteristics, and applications. Biotechnol Appl Biochem. 2022 Dec;69(6):2599-2616. [Content Brief]
[6]. Philipp M, et al. Kinetics of subtilisin and thiolsubtilisin. Mol Cell Biochem. 1983;51(1):5-32. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Subtilisin Related Classifications
HY-P2729 Related Classifications
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