CBN209350
CBN209350 is a selective KDM4 inhibitor with an IC50 of 4 μM. CBN209350 exerts antiproliferative effects on prostate cancer cells. CBN209350 can be used for the research of prostate cancer.
For research use only. We do not sell to patients.
- CAS No.: 726201-10-1
- Formula: C13H12N4O3
- Molecular Weight:272.26
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
In Vitro
CBN209350 exerts inhibitory effects with comparable potency against all tested KDM4 family subtypes, and exhibits moderate selectivity for KDM4 over KDM2A (IC50 120 μM) and PHF8 (IC50 30 μM)[1].
CBN209350 (48 h) exerts cytostatic effects on LnCaP prostate cancer cells[1].
CBN209350 (100 μM; 48 h) reduces the level of H3K9me3 in PC-3 prostate cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
-
Cell Line:Human prostate cancer cell lines LnCaP, DU145, PC-3
-
Concentration:Dose-response titration
-
Incubation Time:48 h
-
Result:Inhibited LnCaP cell viability with an IC50 of 410 μM.
Did not reduce DU145 or PC-3 cell viability by 50% at concentrations up to 1000 μM (IC50 >1000 μM for both cell lines).
-
Cell Line:Human prostate cancer cell lines LnCaP, DU145, PC-3
-
Concentration:100 μM
-
Incubation Time:48 h
-
Result:Did not alter global H3K9me3 levels relative to DMSO-treated control in LnCaP or DU145 cells.
Caused a significant decrease in global H3K9me3 levels relative to DMSO-treated control in PC-3 cells.
Chemical Information
-
CAS No. 726201-10-1
-
Molecular Weight 272.26
-
Formula C13H12N4O3
-
SMILES
O=C(OC)CC1=NN(C(=O)C1)C2=NC=3C=CC=CC3N2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CBN209350 Related Classifications
HY-126207 Related Classifications
Powered by Bioz