Art. -Nr.: HY-15127

Isotretinoin

(Synonyms: 13-cis-Retinoic acid)

13 Cited Publications

Kundenbewertung

Based on 13 publication(s) in Google Scholar

Isotretinoin (13-cis-Retinoic acid) is an orally active vitamin A derivative and is often be used for the research of severe acne. Isotretinoin also shows anticancer activity.

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Reinheit: 99.90%
CAS. Nr.: 4759-48-2
Formel: C₂₀H₂₈O₂
Molecular Weight:300.44

Speicherung:

-20°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Isotretinoin Chemical Structure

CAS. Nr.: 4759-48-2

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (13)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Isotretinoin (13-cis-Retinoic acid) is an orally active vitamin A derivative and is often be used for the research of severe acne. Isotretinoin also shows anticancer activity^[1]^[2].

IC₅₀ & Target^[2]

Human Endogenous Metabolite

Alle RAR/RXR Isoform-spezifische Produkte anzeigen :

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	35.8 μM Compound: 3, 13-cis-RA	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 19231037]
BEL-7404 tumor cell line	IC₅₀	42.6 μM Compound: 3, 13-cis-RA	Cytotoxicity against human Bel7404 cells after 24 hrs by MTT assay Cytotoxicity against human Bel7404 cells after 24 hrs by MTT assay	[PMID: 19231037]
F9	ED₅₀	3.0 x 10^-10M Compound: 2a	Ability to induce differentiation of mouse F9 embryonal carcinoma cells. Ability to induce differentiation of mouse F9 embryonal carcinoma cells.	[PMID: 2836589]
HL-60	ED₅₀	100 nM Compound: 2	Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard. Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard.	[PMID: 1992144]
HL-60	ED₅₀	5.9 x 10^-9M Compound: 2	Concentration required for 50% inhibition of differentiation-inducing activity against human promyelocytic leukemia cell line HL-60 Concentration required for 50% inhibition of differentiation-inducing activity against human promyelocytic leukemia cell line HL-60	[PMID: 1738149]

In Vitro

Isotretinoin (13-cis-Retinoic acid) may act as a pro-drug that is converted intracellularly to metabolites that are agonists for RAR and RXR nuclear receptors^[1].
Isotretinoin is highly sensitive to air, heat, and light^[3].
Isotretinoin (0-40 μM; 24-96 h) significantly inhibits HepG2 cell viability^[4].
Isotretinoin (10 μM; 48 h) down-regulates c-MYC mRNA expression and this is partially due to P1 or P2 promoter activity^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

HepG2
Concentration:

0.1, 0.5, 1, 5, 10, 20, and 40 μM
Incubation Time:

24, 48, 72, and 96 h
Result:

Showed a significant reduction in cell viability (less than 30%) at 96 h for all doses.

RT-PCR^[4]

Cell Line:

HepG2
Concentration:

10 µM
Incubation Time:

48 h
Result:

Significantly reduced the expression of c-MYC mRNA by ~80%.

In Vivo

Isotretinoin (13-cis-Retinoic acid) (1.3-481 μg/L; inhalation exposure; 45 min daily for 2 weeks) is an effective lung cancer chemopreventive agent in A/J Mice^[2].
Isotretinoin accelerates alveolar repair after exodontia in rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male A/J mice, lung carcinogenesis model^[2]
Dosage:

1.3, 20.7, or 481 μg/L
Administration:

Inhalation exposure, 45 min daily for 2 weeks
Result:

Reduced the tumor multiplicity. Caused up-regulation of lung tissue nuclear retinoic acid receptors (RARs) relative to vehicle-exposed mice, RARα (3.9-fold vehicle), RARβ (3.3-fold), and RARγ (3.7-fold) at 20.7 μg/L.

Animal Model:

Wistar rats, tooth extraction model^[3]
Dosage:

7.5 mg/kg
Administration:

Oral gavage, daily for 30 days
Result:

Accelerated the process of alveolar repair, significantly decreased serum calcium levels.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 4759-48-2
Appearance Solid
Molecular Weight 300.44
Formel C₂₀H₂₈O₂
Color Yellow to orange
SMILES

CC1(C)C(/C=C/C(C)=C/C=C/C(C)=C\C(O)=O)=C(C)CCC1
Synonyms

13-cis-Retinoic acid
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (13)

All In Vivo Efficacy StudyCell Proliferation/Viability AssayCell Imaging/StainingRT-PCR

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Journal Impact Factor

Nat Commun

Bone morphogenetic protein (BMP) signaling determines neuroblastoma cell fate and sensitivity to retinoic acid. [Abstract]

2025 Feb 28;16(1):2036. PMID: 40021625
Proc Natl Acad Sci U S A

Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration. [Abstract]

2021 Jan 12;118(2):e2009539118. PMID: 33376206
Br J Cancer

Synergy of retinoic acid and BH3 mimetics in MYC(N)-driven embryonal nervous system tumours. [Abstract]

2024 Sep;131(4):763-777. PMID: 38942989

Isotretinoin purchased from MedChemExpress. Usage Cited in: Br J Cancer. 2024 Sep;131(4):763-777. [Abstract]

Representative zebrafish embryo xenograft images before (Day1) and after 48h (Day3) of treatment and corresponding waterfall plots representing the change from baseline growth of individual NB-S-124 zebrafish embryo neuroblastoma xenografts treated with DMSO (blue), Isotretinoin (500 nM) (red), navitoclax (10 μM) (green), and combination (yellow) for 48 h.
Drug Des Devel Ther

Untargeted Metabolomics Investigating the Intracellular Metabolic Alterations of HaCaT Cells Treated with Isotretinoin. [Abstract]

2026 Feb 18:20:585422. PMID: 41737990
Cell Rep Methods

RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]

2023 Oct 23;3(10):100599. PMID: 37797618
Eur J Pharmacol

Adapalene suppressed the proliferation of melanoma cells by S-phase arrest and subsequent apoptosis via induction of DNA damage. [Abstract]

2019 May 15:851:174-185. PMID: 30836068

Isotretinoin purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2019 May 15:851:174-185. [Abstract]

Isotretinoin (2.5-40 μM; 72 h) exhibited strong cytotoxic effects on the proliferation of M14 cells.
Cell Oncol (Dordr)

Targeting TRIM24 promotes neuroblastoma differentiation and decreases tumorigenicity via LSD1/CoREST complex. [Abstract]

2023 Dec;46(6):1763-1775. PMID: 37466744

Isotretinoin purchased from MedChemExpress. Usage Cited in: Cell Oncol (Dordr). 2023 Dec;46(6):1763-1775. [Abstract]

Isotretinoin (RA) (1-10 μM; 7 d) treatment reduced cell viability in SK-NBE(2) and SH-SY5Y cells.

Isotretinoin purchased from MedChemExpress. Usage Cited in: Cell Oncol (Dordr). 2023 Dec;46(6):1763-1775. [Abstract]

The morphology changes and quantification of neurite lengths of SK-N-BE(2) cells after TRIM24 KO in the absence or presence of Isotretinoin (RA) (10 μM; 7 d).

Isotretinoin purchased from MedChemExpress. Usage Cited in: Cell Oncol (Dordr). 2023 Dec;46(6):1763-1775. [Abstract]

qRT-PCR analysis of the mRNA levels of neural differentiation marker genes upon Isotretinoin (RA) (10 μM; 7 d) treatment in control and TRIM24-KO SK-N-BE(2) cells. Isotretinoin (RA) upregulated the expression of neural differentiation marker genes.
Neuromolecular Med

Spatiotemporal Expression of IRS-1 During Brain Development and its Role in Neural Stem Cell Differentiation. [Abstract]

2025 May 2;27(1):32. PMID: 40314831
Adv Biol (Weinh)

LIN28B Promotes Cancer Cell Dissemination and Angiogenesis. [Abstract]

2025 Jul 18:e00730. PMID: 40679030
Fundam Clin Pharmacol

The third-generation retinoid adapalene triggered DNA damage to induce S-phase arrest in HaCat cells. [Abstract]

2020 Jun;34(3):380-388. PMID: 31808972
J Pediatr Surg

CAMK2G Promotes Neuronal Differentiation and Inhibits Migration in Neuroblastoma. [Abstract]

2025 Feb;60(2):161679. PMID: 39266386
bioRxiv

KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma. [Abstract]

2025 Jul 2:2025.07.01.662644. PMID: 40631259
bioRxiv

Closing the loop: Teaching single-cell foundation models to learn from perturbations. [Abstract]

2025 Jul 12:2025.07.08.663754. PMID: 40672312

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 140 mg/mL (465.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3285 mL

16.6423 mL

33.2845 mL

5 mM

0.6657 mL

3.3285 mL

6.6569 mL

10 mM

0.3328 mL

1.6642 mL

3.3285 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (8.32 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Layton A. The use of isotretinoin in acne. Dermatoendocrinol. 2009 May;1(3):162-9. [Content Brief]

[2]. Dahl AR, et al. Inhaled isotretinoin (13-cis retinoic acid) is an effective lung cancer chemopreventive agent in A/J mice at low doses: a pilot study. Clin Cancer Res. 2000 Aug;6(8):3015-24 [Content Brief]

[3]. Bergoli RD, et al. Isotretinoin effect on alveolar repair after exodontia--a study in rats. Oral Maxillofac Surg. 2011 Jun;15(2):85-92. [Content Brief]

[4]. Ramírez-Flores PN, et al. Isotretinoin and Thalidomide Down-Regulate c-MYC Gene Expression and Modify Proteins Associated with Cancer in Hepatic Cells. Molecules. 2021 Sep 22;26(19):5742. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3285 mL	16.6423 mL	33.2845 mL	83.2113 mL
	5 mM	0.6657 mL	3.3285 mL	6.6569 mL	16.6423 mL
	10 mM	0.3328 mL	1.6642 mL	3.3285 mL	8.3211 mL
	15 mM	0.2219 mL	1.1095 mL	2.2190 mL	5.5474 mL
	20 mM	0.1664 mL	0.8321 mL	1.6642 mL	4.1606 mL
	25 mM	0.1331 mL	0.6657 mL	1.3314 mL	3.3285 mL
	30 mM	0.1109 mL	0.5547 mL	1.1095 mL	2.7737 mL
	40 mM	0.0832 mL	0.4161 mL	0.8321 mL	2.0803 mL
	50 mM	0.0666 mL	0.3328 mL	0.6657 mL	1.6642 mL
	60 mM	0.0555 mL	0.2774 mL	0.5547 mL	1.3869 mL
	80 mM	0.0416 mL	0.2080 mL	0.4161 mL	1.0401 mL
	100 mM	0.0333 mL	0.1664 mL	0.3328 mL	0.8321 mL

Keywords

Isotretinoin

13 Cited Publications

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Isotretinoin

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Publications Citing Use of MedChemExpress (MCE) Isotretinoin

Alle RAR/RXR Isoform-spezifische Produkte anzeigen

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (13)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Isotretinoin

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Publications Citing Use of MedChemExpress (MCE) Isotretinoin

Alle RAR/RXR Isoform-spezifische Produkte anzeigen

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (13)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Isotretinoin Related Classifications

HY-15127 Related Classifications