Art. -Nr.: HY-108496

Sphingosine-1-phosphate

(Synonyms: S1P)

21 Cited Publications

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Based on 21 publication(s) in Google Scholar

Sphingosine-1-phosphate (S1P) is an agonist of S1P_1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca²⁺ as an extracellular ligand for G protein-coupled receptors. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membrane phospholipids. Sphingosine-1-phosphate stimulates the DNA synthesis, cell proliferation and migration.

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Reinheit: 99.94%
CAS. Nr.: 26993-30-6
Formel: C₁₈H₃₈NO₅P
Molecular Weight:379.47

Speicherung:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Sphingosine-1-phosphate Chemical Structure

CAS. Nr.: 26993-30-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (21)
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- In Vitro
- In Vivo
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Biologische Aktivität

Beschreibung

Sphingosine-1-phosphate (S1P) is an agonist of S1P_1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca²⁺ as an extracellular ligand for G protein-coupled receptors^[1]. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membrane phospholipids^[2]. Sphingosine-1-phosphate stimulates the DNA synthesis, cell proliferation and migration^[4].

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Alle LPL Receptor Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	0.04 nM Compound: S1P	Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 3 expressed on CHO cell membranes Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 3 expressed on CHO cell membranes	[PMID: 15149705]
CHO	IC₅₀	0.16 nM Compound: S1P	Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 1 expressed on CHO cell membranes Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 1 expressed on CHO cell membranes	[PMID: 15149705]
CHO	IC₅₀	0.23 nM Compound: S1P	Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 5 expressed on CHO cell membranes Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 5 expressed on CHO cell membranes	[PMID: 15149705]
CHO	IC₅₀	23 nM Compound: S1P	Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 4 expressed on CHO cell membranes Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 4 expressed on CHO cell membranes	[PMID: 15149705]
CHO	IC₅₀	0.26 nM Compound: S1P	Displacement of [33P]sphingosine 1 phosphate from human S1P3 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P3 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	0.35 nM Compound: S1P	Displacement of [33P]sphingosine 1 phosphate from human S1P2 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P2 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	0.55 nM Compound: S1P	Displacement of [33P]sphingosine 1 phosphate from human S1P5 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P5 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	0.67 nM Compound: S1P	Displacement of [33P]sphingosine 1 phosphate from human S1P1 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P1 receptor expressed in CHO cells	[PMID: 15615513]
CHO	IC₅₀	34 nM Compound: S1P	Displacement of [33P]sphingosine 1 phosphate from human S1P4 receptor expressed in CHO cells Displacement of [33P]sphingosine 1 phosphate from human S1P4 receptor expressed in CHO cells	[PMID: 15615513]
CHO	EC₅₀	0.55 nM Compound: sphingosine-P	Activity at human recombinant S1P1 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay Activity at human recombinant S1P1 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay	[PMID: 17070046]
CHO	EC₅₀	0.91 nM Compound: sphingosine-P	Activity at human recombinant S1P5 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay Activity at human recombinant S1P5 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay	[PMID: 17070046]
CHO	EC₅₀	1.6 nM Compound: sphingosine-P	Activity at human recombinant S1P3 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay Activity at human recombinant S1P3 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay	[PMID: 17070046]
CHO	EC₅₀	7.9 nM Compound: sphingosine-P	Activity at human recombinant S1P4 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay Activity at human recombinant S1P4 receptor expressed in CHO cells assessed as increase in calcium release by FLIPR assay	[PMID: 17070046]
CHO	EC₅₀	0.2 nM Compound: S1P	Displacement of [33P]S1P from human recombinant S1P5 receptor expressed in CHO cells by scintillation counting Displacement of [33P]S1P from human recombinant S1P5 receptor expressed in CHO cells by scintillation counting	[PMID: 20446681]
CHO	EC₅₀	121 nM Compound: S1P	Agonist activity at human recombinant S1P5 receptor expressed in CHO cells by GTPgammaS binding assay Agonist activity at human recombinant S1P5 receptor expressed in CHO cells by GTPgammaS binding assay	[PMID: 20446681]
CHO	EC₅₀	164 nM Compound: S1P	Agonist activity at human recombinant S1P4 receptor expressed in CHO cells by GTPgammaS binding assay Agonist activity at human recombinant S1P4 receptor expressed in CHO cells by GTPgammaS binding assay	[PMID: 20446681]
CHO	IC₅₀	2.9 nM Compound: S1P	Displacement of [33P]S1P from human recombinant S1P4 receptor expressed in CHO cells by scintillation counting Displacement of [33P]S1P from human recombinant S1P4 receptor expressed in CHO cells by scintillation counting	[PMID: 20446681]
HEK293	EC₅₀	0.1 nM Compound: S1P	Displacement of [33P]S1P from human recombinant S1P1 receptor expressed in HEK cells by scintillation counting Displacement of [33P]S1P from human recombinant S1P1 receptor expressed in HEK cells by scintillation counting	[PMID: 20446681]
HEK293	EC₅₀	0.2 nM Compound: S1P	Displacement of [33P]S1P from human recombinant S1P3 receptor expressed in HEK cells by scintillation counting Displacement of [33P]S1P from human recombinant S1P3 receptor expressed in HEK cells by scintillation counting	[PMID: 20446681]
HEK293	EC₅₀	0.7 nM Compound: S1P	Agonist activity at human recombinant S1P3 receptor expressed in HEK cells by GTPgammaS binding assay Agonist activity at human recombinant S1P3 receptor expressed in HEK cells by GTPgammaS binding assay	[PMID: 20446681]
HEK293	EC₅₀	0.7 nM Compound: S1P	Displacement of [33P]S1P from human recombinant S1P2 receptor expressed in HEK cells by scintillation counting Displacement of [33P]S1P from human recombinant S1P2 receptor expressed in HEK cells by scintillation counting	[PMID: 20446681]
HEK293	EC₅₀	25.3 nM Compound: S1P	Agonist activity at human recombinant S1P1 receptor expressed in HEK cells by GTPgammaS binding assay Agonist activity at human recombinant S1P1 receptor expressed in HEK cells by GTPgammaS binding assay	[PMID: 20446681]
HEK293	EC₅₀	43.9 nM Compound: S1P	Agonist activity at human recombinant S1P2 receptor expressed in HEK cells by GTPgammaS binding assay Agonist activity at human recombinant S1P2 receptor expressed in HEK cells by GTPgammaS binding assay	[PMID: 20446681]
HEK-293T	EC₅₀	0.9 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 1 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 1 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1)	[PMID: 14505636]
HEK-293T	EC₅₀	1.1 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 3 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 3 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1)	[PMID: 14505636]
HEK-293T	EC₅₀	2.9 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 2 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 2 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1)	[PMID: 14505636]
HEK-293T	EC₅₀	270 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 4 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 4 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2)	[PMID: 14505636]
HEK-293T	EC₅₀	4.5 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 1 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand. (Experiment 2) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 1 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand. (Experiment 2)	[PMID: 14505636]
HEK-293T	EC₅₀	43.9 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 5 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 5 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 1)	[PMID: 14505636]
HEK-293T	EC₅₀	8.3 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 2 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 2 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2)	[PMID: 14505636]
HEK-293T	EC₅₀	8.7 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 3 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 3 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2)	[PMID: 14505636]
HEK-293T	EC₅₀	9.2 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 5 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2) In vitro binding affinity towards human Sphingosine 1-phosphate receptor 5 expressed in HEK293T cells using [gamma-35S]-GTP as radioligand (Experiment 2)	[PMID: 14505636]
HEK-293T	EC₅₀	1.2 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 3 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand In vitro binding affinity towards human Sphingosine 1-phosphate receptor 3 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand	[PMID: 15341948]
HEK-293T	EC₅₀	1.7 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 5 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand In vitro binding affinity towards human Sphingosine 1-phosphate receptor 5 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand	[PMID: 15341948]
HEK-293T	EC₅₀	2.2 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 2 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand In vitro binding affinity towards human Sphingosine 1-phosphate receptor 2 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand	[PMID: 15341948]
HEK-293T	EC₅₀	20 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 1 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand In vitro binding affinity towards human Sphingosine 1-phosphate receptor 1 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand	[PMID: 15341948]
HEK-293T	EC₅₀	37 nM Compound: S1P	In vitro binding affinity towards human Sphingosine 1-phosphate receptor 4 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand In vitro binding affinity towards human Sphingosine 1-phosphate receptor 4 expressed in HEK293T cells was determined using [gamma-35S]-GTP as radioligand	[PMID: 15341948]
HEK-293T	EC₅₀	2.4 nM Compound: S1P	Antagonist activity at human S1P3 receptor expressed in HEK293T cells assessed as inhibition of sphingosine-1-phosphate-induced gamma-[35S]GTP binding after 30 mins by scintillation counting Antagonist activity at human S1P3 receptor expressed in HEK293T cells assessed as inhibition of sphingosine-1-phosphate-induced gamma-[35S]GTP binding after 30 mins by scintillation counting	[PMID: 20304639]
HEK-293T	EC₅₀	5.6 nM Compound: S1P	Antagonist activity at human S1P1 receptor expressed in HEK293T cells assessed as inhibition of sphingosine-1-phosphate-induced gamma-[35S]GTP binding after 30 mins by scintillation counting Antagonist activity at human S1P1 receptor expressed in HEK293T cells assessed as inhibition of sphingosine-1-phosphate-induced gamma-[35S]GTP binding after 30 mins by scintillation counting	[PMID: 20304639]
HEK-293T	IC₅₀	0.78 nM Compound: S1P	Displacement of [33P]sphingosine-1-phosphate from human S1P1 receptor expressed in HEK293T cells after 60 mins by scintillation counting Displacement of [33P]sphingosine-1-phosphate from human S1P1 receptor expressed in HEK293T cells after 60 mins by scintillation counting	[PMID: 20304639]
HEK-293T	IC₅₀	0.92 nM Compound: S1P	Displacement of [33P]sphingosine-1-phosphate from human S1P3 receptor expressed in HEK293T cells after 60 mins by scintillation counting Displacement of [33P]sphingosine-1-phosphate from human S1P3 receptor expressed in HEK293T cells after 60 mins by scintillation counting	[PMID: 20304639]
HEK-293T	IC₅₀	1.04 nM Compound: S1P	Displacement of [33P]sphingosine-1-phosphate from human S1P4 receptor expressed in HEK293T cells after 60 mins by scintillation counting Displacement of [33P]sphingosine-1-phosphate from human S1P4 receptor expressed in HEK293T cells after 60 mins by scintillation counting	[PMID: 20304639]
HEK-293T	IC₅₀	2 nM Compound: S1P	Displacement of [33P]sphingosine-1-phosphate from human S1P5 receptor expressed in HEK293T cells after 60 mins by scintillation counting Displacement of [33P]sphingosine-1-phosphate from human S1P5 receptor expressed in HEK293T cells after 60 mins by scintillation counting	[PMID: 20304639]
HEK-293T	EC₅₀	2.4 nM Compound: S1P	Agonist activity at human S1P3 receptor expressed in HEK293T cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human S1P3 receptor expressed in HEK293T cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 23245510]
HEK-293T	EC₅₀	5.6 nM Compound: S1P	Agonist activity at human S1P1 receptor expressed in HEK293T cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human S1P1 receptor expressed in HEK293T cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 23245510]
HEK-293T	EC₅₀	1.3 nM Compound: S1P	Agonist activity at human S1P2R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting Agonist activity at human S1P2R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting	[PMID: 24900589]
HEK-293T	EC₅₀	1.8 nM Compound: S1P	Agonist activity at human S1P5R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting Agonist activity at human S1P5R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting	[PMID: 24900589]
HEK-293T	EC₅₀	13 nM Compound: S1P	Agonist activity at human S1P4R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting Agonist activity at human S1P4R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting	[PMID: 24900589]
HEK-293T	EC₅₀	3.94 nM Compound: S1P	Agonist activity at human S1P3R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting Agonist activity at human S1P3R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting	[PMID: 24900589]
HEK-293T	EC₅₀	4.25 nM Compound: S1P	Agonist activity at human S1P1R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting Agonist activity at human S1P1R expressed in HEK293T cells assessed as [35S]GTPgammaS binding after 30 mins by scintillation counting	[PMID: 24900589]
U2OS	EC₅₀	0.018 μM Compound: 1, S1P	Agonist activity at human S1P1 receptor expressed in human U2OS cells co-expressing eGFP assessed as receptor internalization into cytoplasm using Hoechst dye staining Agonist activity at human S1P1 receptor expressed in human U2OS cells co-expressing eGFP assessed as receptor internalization into cytoplasm using Hoechst dye staining	[PMID: 22104144]
U2OS	EC₅₀	2.8 nM Compound: S1P	Agonist activity at human S1P1 receptor expressed in EDG1-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay Agonist activity at human S1P1 receptor expressed in EDG1-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay	[PMID: 26687487]
U2OS	EC₅₀	79 nM Compound: S1P	Agonist activity at human S1P3 receptor expressed in EDG3-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay Agonist activity at human S1P3 receptor expressed in EDG3-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay	[PMID: 26687487]

In Vitro

S1P (1 μM) induces a significant Ca²⁺ releases in HEK293 cells under serum starvation conditions (1% FCS)^[1].
In a functional Ca²⁺ assay, Suramin (HY-B0879) alone does not exert any effect on intracellular Ca²⁺ release via gpr3, gpr6 or gpr12. In contrast, S1P (1 μM) induces Ca²⁺ release of gpr3, gpr6 and gpr12 in the presence of Suramin (HY-B0879) various concentrations in transfected HEK293 cells^[2].
In a functional Ca²⁺ assay,S1P (3-3000 nM) in the presence Suramin (300 μM), exhibits nanomolar EC₅₀ values for gpr3 (EC₅₀=29 nM), gpr6 (EC₅₀=15 nM) and gpr12 (EC₅₀=24 nM), rat gpr3 (EC₅₀=68 nM),respectively in HEK293 cells^[2].
S1P increases intracellular calcium levels in TAg-Jurkat cells expressing S1P1 and G_qi5, which allows for phospholipase C stimulation by G_i proteins, when used at a concentration of 200 nM, as well as in TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC₅₀s = 8 and 11 nM, respectively)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 26993-30-6
Appearance Solid
Molecular Weight 379.47
Formel C₁₈H₃₈NO₅P
Color White to yellow
SMILES

N[C@@H](CO[P](O)(O)=O)[C@H](O)/C=C/CCCCCCCCCCCCC
Synonyms

S1P
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (21)

All Flow CytometryBio/Physico-chemical AssayRT-PCRCell Migration/Invasion AssayWBCell Imaging/Staining

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Journal Impact Factor

Mol Cancer

EGFR-mediated local invasiveness and response to Cetuximab in head and neck cancer. [Abstract]

2025 Mar 22;24(1):94. PMID: 40121428
Cell Metab

Adipokine IL-11/IL-11Ra constrains sphingolipid metabolism to limit the thermogenic capacity of beige adipocytes. [Abstract]

2026 May 14:S1550-4131(26)00151-8. PMID: 42140185
Cell Death Differ

Efferocytosis enhances macrophage pro-angiogenic functions for bone marrow regeneration. [Abstract]

2026 Jun 24. PMID: 42337094
Adv Sci (Weinh)

A Microbial Lipid-ATP Synthase Axis Fuels NK Cell Antitumor Activity. [Abstract]

2026 Jul;13(39):e20095. PMID: 41972457
Sci Adv

In situ-formed immunotherapeutic hydrogel containing sphingosine-1-phosphate for enhanced lung cancer immunotherapy. [Abstract]

2025 Aug 8;11(32):eadw5001. PMID: 40779634

Sphingosine-1-phosphate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 8;11(32):eadw5001. [Abstract]

Representative flow cytometric plots and quantitative analysis of mature DCs (CD80⁺CD86⁺) gating on CD11c⁺ cells after treated with different concentrations of S1P (0.5-1.0 μM; 24 h).

Sphingosine-1-phosphate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 8;11(32):eadw5001. [Abstract]

The levels of lactate dehydrogenase (LDH) after coculture of S1P-treated (0.5-1.0 μM; 24 h) BMDMs and LLC cells at radios of 10:1 or 15:1.

Sphingosine-1-phosphate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 8;11(32):eadw5001. [Abstract]

Relative mRNA expression of M1 macrophages marker genes (iNOS, IL-6, IL-1β, and TNF-α) in S1P-treated (0.5-1.0 μM; 24 h) BMDMs.

Sphingosine-1-phosphate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 8;11(32):eadw5001. [Abstract]

Representative images of migratory Raw264.7 cells stained with crystal violet at 18 hours after incubation with S1P (0.5-1.0 μM).

Sphingosine-1-phosphate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 8;11(32):eadw5001. [Abstract]

The levels of p-STAT1, p-p65, and p-IκBα expressed in BMDMs after treated with 1 μM S1P for 15, 30, or 120 min.

Sphingosine-1-phosphate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 8;11(32):eadw5001. [Abstract]

The levels of NLRP3 expressed in BMDMs after treated with 1 μM S1P for 4 or 8 hours.

Sphingosine-1-phosphate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 8;11(32):eadw5001. [Abstract]

Mitochondrial ROS and mitochondria in Raw264.7 were detected by MitoSOX Red staining and TMRM, respectively, and analyzed by confocal microscopy after S1P (1 μM; 24 h) and Mdivi-1 (10 μM) treatment.
J Neuroinflammation

Accumulated BCAAs and BCKAs contribute to the HFD-induced deterioration of Alzheimer's disease via a dysfunctional TREM2-related reduction in microglial β-amyloid clearance. [Abstract]

2024 Dec 23;21(1):327. PMID: 39716292
Genome Biol

Single-cell transcriptomics reveals multiple chemoresistant properties in leukemic stem and progenitor cells in pediatric AML. [Abstract]

2023 Aug 31;24(1):199. PMID: 37653425
Cell Commun Signal

Concomitant targeting of FLT3 and SPHK1 exerts synergistic cytotoxicity in FLT3-ITD+ acute myeloid leukemia by inhibiting β-catenin activity via the PP2A-GSK3β axis. [Abstract]

2024 Aug 7;22(1):391. PMID: 39113090
Front Immunol

Sphingosine-1-phosphate alleviates colitis by regulating macrophage polarization and PI3k-Akt signaling. [Abstract]

2025 Jul 21:16:1622094. PMID: 40761802
Neurobiol Dis

Metabolomics-driven exploration of sphingosine 1-phosphate mechanisms in refractory epilepsy. [Abstract]

2025 May 9:106953. PMID: 40349856
Cell Prolif

MCL restrained ROS/AKT/ASAH1 pathway to therapy tamoxifen resistance breast cancer by stabilizing NRF2. [Abstract]

2024 Jun 26:e13700. PMID: 38924190
Chem Biol Interact

S1P/S1PR2 promote pancreatic stellate cell activation and pancreatic fibrosis in chronic pancreatitis by regulating autophagy and the NLRP3 inflammasome. [Abstract]

2023 Aug 1:380:110541. PMID: 37169277
Stem Cell Reports

YAP signaling in horizontal basal cells promotes the regeneration of olfactory epithelium after injury. [Abstract]

2022 Mar 8;17(3):664-677. PMID: 35148842
Eur J Pharmacol

Vitexin attenuates neuropathic pain by regulating astrocyte autophagy flux and polarization via the S1P/ S1PR1-PI3K/ Akt axis. [Abstract]

2024 Jul 31:176848. PMID: 39094925
FASEB J

SPHK1/S1P/S1PR pathway promotes the progression of peritoneal fibrosis by mesothelial-mesenchymal transition. [Abstract]

2024 Jan 31;38(2):e23417. PMID: 38226856
Metabolites

Transcriptome and Lipidomic Analysis Suggests Lipid Metabolism Reprogramming and Upregulating SPHK1 Promotes Stemness in Pancreatic Ductal Adenocarcinoma Stem-like Cells. [Abstract]

2023 Nov 4;13(11):1132. PMID: 37999228
J Cancer

Tumor cell-derived hyaluronan fragments induce endocytosis of S1PR1 to promote lymphangiogenesis through LYVE-1-Src pathway. [Abstract]

2025 Jan 27;16(5):1466-1478. PMID: 39991586
Ren Fail

Hypoxia-inducible factor 2α overexpression in podocytes ameliorates lipid metabolism disorders in diabetic kidney disease by inhibiting S1P. [Abstract]

2025 Dec;47(1):2568972. PMID: 41077842
J Nat Med

Chaihu Guizhi Ganjiang Decoction ameliorates chronic pancreatitis by modulating the SK1/S1P signaling pathway. [Abstract]

2025 Apr 3. PMID: 40178769
Neurosci Lett

Sphingosine-1-phosphate attenuates seizures and cognitive deficits, associated with reduced hippocampal IL-1β in a PTZ-induced epilepsy model. [Abstract]

2026 Mar 26:875:138527. PMID: 41621476
bioRxiv

2026 Apr 30.

Lösungsmittel & Löslichkeit

In Vitro:

1 M NaOH : 33.33 mg/mL (87.83 mM; Need ultrasonic)

Methanol : 3.85 mg/mL (10.15 mM; ultrasonic and warming and adjust pH to 3 with 1M HCl and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6353 mL

13.1763 mL

26.3525 mL

5 mM

0.5271 mL

2.6353 mL

5.2705 mL

10 mM

0.2635 mL

1.3176 mL

2.6353 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.1% BSA in Saline

Solubility: 0.5 mg/mL (1.32 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Teresa Sanchez, et al. Structural and Functional Characteristics of S1P Receptors. J Cell Biochem. 2004 Aug 1;92(5):913-22. [Content Brief]

[2]. Kirsten Uhlenbrock, et al. Sphingosine 1-phosphate Is a Ligand of the Human gpr3, gpr6 and gpr12 Family of Constitutively Active G Protein-Coupled Receptors. Cell Signal. 2002 Nov;14(11):941-53. [Content Brief]

[3]. S An, et al. Transduction of Intracellular Calcium Signals Through G Protein-Mediated Activation of Phospholipase C by Recombinant Sphingosine 1-phosphate Receptors. Mol Pharmacol. 1999 May;55(5):787-94 [Content Brief]

[4]. Calise S, et al., Sphingosine 1-phosphate stimulates proliferation and migration of satellite cells: role of S1P receptors. Biochim Biophys Acta. 2012 Feb;1823(2):439-50. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Methanol / 1 M NaOH	1 mM	2.6353 mL	13.1763 mL	26.3525 mL	65.8814 mL
	5 mM	0.5271 mL	2.6353 mL	5.2705 mL	13.1763 mL
	10 mM	0.2635 mL	1.3176 mL	2.6353 mL	6.5881 mL
1 M NaOH	15 mM	0.1757 mL	0.8784 mL	1.7568 mL	4.3921 mL
	20 mM	0.1318 mL	0.6588 mL	1.3176 mL	3.2941 mL
	25 mM	0.1054 mL	0.5271 mL	1.0541 mL	2.6353 mL
	30 mM	0.0878 mL	0.4392 mL	0.8784 mL	2.1960 mL
	40 mM	0.0659 mL	0.3294 mL	0.6588 mL	1.6470 mL
	50 mM	0.0527 mL	0.2635 mL	0.5271 mL	1.3176 mL
	60 mM	0.0439 mL	0.2196 mL	0.4392 mL	1.0980 mL
	80 mM	0.0329 mL	0.1647 mL	0.3294 mL	0.8235 mL

Keywords

Sphingosine-1-phosphate

21 Cited Publications

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Sphingosine-1-phosphate

Publications Citing Use of MedChemExpress (MCE) Sphingosine-1-phosphate

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Alle LPL Receptor Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (21)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Sphingosine-1-phosphate Related Classifications

HY-108496 Related Classifications