Art. -Nr.: HY-153508

ANO1-IN-4

Kundenbewertung

Based on 1 Customer Validation

ANO1-IN-4 (Compound 10bm) is a reversible inhibitor for calcium-activated chloride channel transmembrane protein 16A (TMEM16A, also known as ANO1) with an IC₅₀ of 0.030 µM. ANO1-IN-4 exhibits good metabolic stability in rat liver microsomes. ANO1-IN-4 inhibits spontaneous contraction in mouse isolated ileum.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.58%
CAS. Nr.: 2098490-06-1
Formel: C₁₉H₁₉BrF₂N₂O₂S
Molecular Weight:457.33

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

ANO1-IN-4 Chemical Structure

CAS. Nr.: 2098490-06-1

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC₅₀: 0.030 µM (ANO1)

Cellular Effect

Cell Line	Type	Value	Description	References
U138-MG	GI₅₀	29.4 μM Compound: 4	Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-251	GI₅₀	45.33 μM Compound: 4	Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-87MG ATCC	GI₅₀	48 μM Compound: 4	Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]

Chemical Information

CAS. Nr. 2098490-06-1
Appearance Solid
Molecular Weight 457.33
Formel C₁₉H₁₉BrF₂N₂O₂S
Color White to off-white
SMILES

O=C(C1=C(NC(C(F)(Br)F)=O)SC2=C1CCCCC2)NC3=C(C)C=CC=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (109.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1866 mL

10.9330 mL

21.8660 mL

5 mM

0.4373 mL

2.1866 mL

4.3732 mL

10 mM

0.2187 mL

1.0933 mL

2.1866 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Truong EC, et al., Substituted 2-Acylaminocycloalkylthiophene-3-carboxylic Acid Arylamides as Inhibitors of the Calcium-Activated Chloride Channel Transmembrane Protein 16A (TMEM16A). J Med Chem. 2017 Jun 8;60(11):4626-4635. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1866 mL	10.9330 mL	21.8660 mL	54.6651 mL
	5 mM	0.4373 mL	2.1866 mL	4.3732 mL	10.9330 mL
	10 mM	0.2187 mL	1.0933 mL	2.1866 mL	5.4665 mL
	15 mM	0.1458 mL	0.7289 mL	1.4577 mL	3.6443 mL
	20 mM	0.1093 mL	0.5467 mL	1.0933 mL	2.7333 mL
	25 mM	0.0875 mL	0.4373 mL	0.8746 mL	2.1866 mL
	30 mM	0.0729 mL	0.3644 mL	0.7289 mL	1.8222 mL
	40 mM	0.0547 mL	0.2733 mL	0.5467 mL	1.3666 mL
	50 mM	0.0437 mL	0.2187 mL	0.4373 mL	1.0933 mL
	60 mM	0.0364 mL	0.1822 mL	0.3644 mL	0.9111 mL
	80 mM	0.0273 mL	0.1367 mL	0.2733 mL	0.6833 mL
	100 mM	0.0219 mL	0.1093 mL	0.2187 mL	0.5467 mL

Keywords

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

ANO1-IN-4

Membrane Transporter/Ion Channel Compound Library

Mechanoreceptors Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ANO1-IN-4

Membrane Transporter/Ion Channel Compound Library

Mechanoreceptors Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ANO1-IN-4 Related Classifications

HY-153508 Related Classifications