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Art. -Nr.: HY-135123

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DC4SMe

DC4SMe, a phosphate proagent of cytotoxic DNA alkylator DC4, can be used in the synthesis of antibody-drug conjugate (ADC). DC4SMe exhibits IC₅₀s of 1.9 nM, 2.9 nM, and 1.8 nM for Ramos, Namalwa, and HL60/s cancer cells, respectively. DC4SMe can be used for the targeted treatment of cancer.

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CAS. Nr.: 615538-47-1
Formel: C₃₅H₃₁ClN₅O₇PS₂
Molecular Weight:764.21

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (267 KB)

DC4SMe Chemical Structure

CAS. Nr.: 615538-47-1

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Protokoll (1)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[1]

Duocarmycins

Alle ADC Payloads Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
COLO 205	IC₅₀	>3000 pM Compound: 38, DC4SMe	Cytotoxicity against human COLO205 cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human COLO205 cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
HL-60	IC₅₀	1800 pM Compound: 38, DC4SMe	Cytotoxicity against human HL60 cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human HL60 cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
NAMALVA	IC₅₀	2900 pM Compound: 38, DC4SMe	Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
NAMALVA	IC₅₀	30 pM Compound: 38, DC4SMe	Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay	[PMID: 22148292]
Ramos	IC₅₀	1900 pM Compound: 38, DC4SMe	Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
Ramos	IC₅₀	80 pM Compound: 38, DC4SMe	Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay	[PMID: 22148292]

In Vitro

The phosphate prodrug may be a suitable choice for ADCs of effector molecules that do not possess sufficient water solubility or stability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS. Nr. 615538-47-1
Molecular Weight 764.21
Formel C₃₅H₃₁ClN₅O₇PS₂
SMILES

O=C(C(N1)=CC2=C1C=CC(NC(CCSSC)=O)=C2)NC3=CC4=C(NC(C(N5C[C@@H](CCl)C6=C5C=C(OP(O)(O)=O)C7=CC=CC=C67)=O)=C4)C=C3
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Protokoll

All Cell Biology

Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (267 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Zhao RY, et al. Synthesis and biological evaluation of antibody conjugates of phosphate prodrugs of cytotoxic DNA alkylators for the targeted treatment of cancer. J Med Chem. 2012 Jan 26;55(2):766-82. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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