Euphomilone A
Euphomilone A is an ent-abietane-type diterpenoid osteoclastogenesis inhibitor and RANK inhibitor, found in the aerial parts of Euphorbia milii, possessing a 7/5/6 tricyclic system. Euphomilone A inhibits RANKL-induced osteoclastogenesis in monocyte/macrophage lineage cells. Euphomilone A can be used in research on bone resorption-related diseases.
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- CAS. Nr.: 2077182-79-5
- Formel: C20H32O3
- Molecular Weight:320.47
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| RAW264.7 | IC50 |
12.6 μM
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Inhibition of RANKL-induced osteoclast formation in RAW 264.7 cells.
Inhibition of RANKL-induced osteoclast formation in RAW 264.7 cells.
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acs.orglett.6b03142 |
In Vitro
Euphomilone A inhibits RANKL-induced osteoclast formation in RAW 264.7 cells with an IC50 of 12.6 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 2077182-79-5
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Molecular Weight 320.47
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Formel C20H32O3
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SMILES
C[C@@]12[C@@]3([C@@](C(C(C)([C@@H](CC3)O)C)=O)([H])C[C@@]1([H])C[C@](C)(CC2)C=C)O
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Phagocytosis Functional Assay
A phagocytosis functional assay measures the ability of phagocytic cells, such as neutrophils, macrophages, monocytes, or microglia/macrophages, to bind and internalize particulate targets including bacteria, yeast particles, beads, or myelin particles. Fluorescent flow-cytometry assays detect target uptake as fluorescence associated with gated phagocytes, while pH-sensitive dyes such as pHrodo increase signal in acidic phagosomal compartments and therefore preferentially report internalized particles rather than particles remaining outside the cell. Microscopy or high-content imaging can be used to confirm intracellular localization and, in some protocols, to follow uptake kinetics.
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Osteoclast differentiation from monocyte/macrophage precursors
Osteoclast differentiation is an in vitro induction assay in which monocyte/macrophage-lineage precursors are exposed to macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL), generating multinucleated osteoclasts that are commonly identified by tartrate-resistant acid phosphatase (TRAP) staining and functionally confirmed by resorption pits on dentin, bone, or mineralized substrates. M-CSF supports survival and expansion of osteoclast precursors, while RANKL binding to RANK drives osteoclast commitment, fusion, maturation, and resorptive function; osteoprotegerin inhibits this pathway by binding RANKL and preventing RANK activation. The main readouts are the number of TRAP-positive multinucleated cells, formation of F-actin rings, and resorbed surface area; TRAP-positive multinucleated cells indicate osteoclast differentiation, whereas pit formation on dentin, bone, or mineralized coating indicates functional bone-resorbing activity.
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Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Euphomilone A Related Classifications
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