Monobenzyl phthalate
Based on 1 Customer Validation
Monobenzyl phthalate is a phthalate metabolite (aromatic alkyl chain monophthalate) with oral activity, which acts as a male reproductive development inhibitor and antiandrogen in rat fetuses.
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- Reinheit: 99.88%
- CAS. Nr.: 2528-16-7
- Formel: C15H12O4
- Molecular Weight:256.26
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Chemical Information
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Biologische Aktivität
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar (virgin female, ~14 weeks old, mated with same-strain males, pregnant)[2]
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Dosage:167 mg/kg; 250 mg/kg; 375 mg/kg
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Administration:p.o.; once daily; gestational days 15-17
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Result:Showed no significant changes in fetal body weight, male anogenital distance (AGD), AGD-to-cube root of body weight ratio, or incidence of undescended testes at 167 mg/kg relative to controls.
Caused a significant decrease in AGD and AGD-to-cube root of body weight ratio in male fetuses; observed undescended testes in 21 male fetuses (12 litters) with a significantly increased degree of transabdominal testicular ascent (mean 23.8 units) at 250 mg/kg, with no significant reduction in fetal body weights.
Induced a significant decrease in AGD (to female-like levels) and AGD-to-cube root of body weight ratio in male fetuses; observed undescended testes in 79 male fetuses (16 litters) with a significantly increased degree of transabdominal testicular ascent (mean 40.1 units), and significantly reduced male and female fetal body weights relative to controls at 375 mg/kg.
Left female fetal AGD and AGD-to-cube root of body weight ratio unchanged across all doses tested.
Chemical Information
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CAS. Nr. 2528-16-7
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Appearance Solid
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Molecular Weight 256.26
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Formel C15H12O4
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Color White to off-white
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SMILES
O=C(C1=CC=CC=C1C(O)=O)OCC2=CC=CC=C2
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Synonyms
2-((Benzyloxy)carbonyl)benzoic acid
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (390.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Tian M, et al. Positive association of low-level environmental phthalate exposure with sperm motility was mediated by DNA methylation: A pilot study. Chemosphere. 2019;220:459-467. [Content Brief]
[2]. Ema M, et al. Decreased anogenital distance and increased incidence of undescended testes in fetuses of rats given monobenzyl phthalate, a major metabolite of butyl benzyl phthalate. Reprod Toxicol. 2003;17(4):407-412. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.9023 mL | 19.5114 mL | 39.0229 mL | 97.5572 mL |
| 5 mM | 0.7805 mL | 3.9023 mL | 7.8046 mL | 19.5114 mL | |
| 10 mM | 0.3902 mL | 1.9511 mL | 3.9023 mL | 9.7557 mL | |
| 15 mM | 0.2602 mL | 1.3008 mL | 2.6015 mL | 6.5038 mL | |
| 20 mM | 0.1951 mL | 0.9756 mL | 1.9511 mL | 4.8779 mL | |
| 25 mM | 0.1561 mL | 0.7805 mL | 1.5609 mL | 3.9023 mL | |
| 30 mM | 0.1301 mL | 0.6504 mL | 1.3008 mL | 3.2519 mL | |
| 40 mM | 0.0976 mL | 0.4878 mL | 0.9756 mL | 2.4389 mL | |
| 50 mM | 0.0780 mL | 0.3902 mL | 0.7805 mL | 1.9511 mL | |
| 60 mM | 0.0650 mL | 0.3252 mL | 0.6504 mL | 1.6260 mL | |
| 80 mM | 0.0488 mL | 0.2439 mL | 0.4878 mL | 1.2195 mL | |
| 100 mM | 0.0390 mL | 0.1951 mL | 0.3902 mL | 0.9756 mL |
Monobenzyl phthalate Related Classifications
HY-W011848 Related Classifications
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- Monobenzyl phthalate
- 2528-16-7
- 2-((Benzyloxy)carbonyl)benzoic acid
- Drug Metabolite
- male reproductive development
- rat fetuses
- maternal rat
- yeast estrogenic activity screens
- Wistar rats
- antiandrogenic agent
- butyl benzyl phthalate
- sperm quality parameters
- anogenital distance
- undescended testes
- Inhibitor
- inhibitor
- inhibit