Art. -Nr.: HY-N2387

Pinosylvin

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Pinosylvin is a?pre-infectious stilbenoid toxin?isolated from the heartwood of Pinus species, has anti-bacterial activities. Pinosylvin is a resveratrol analogue, can induce cell apoptosis and autophapy in leukemia cells.

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Reinheit: 99.94%
CAS. Nr.: 22139-77-1
Formel: C₁₄H₁₂O₂
Molecular Weight:212.24

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Pinosylvin Chemical Structure

CAS. Nr.: 22139-77-1

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Pinosylvin is a?pre-infectious stilbenoid toxin?isolated from the heartwood of Pinus species, has anti-bacterial activities^[1]. Pinosylvin is a resveratrol analogue, can induce cell apoptosis and autophapy in leukemia cells^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	>10 μM Compound: 1	Antagonist activity at human TRPV1 expressed in HEK293 cells assessed as inhibition of allyl isothiocyanate-induced increase of intracellular calcium level Antagonist activity at human TRPV1 expressed in HEK293 cells assessed as inhibition of allyl isothiocyanate-induced increase of intracellular calcium level	[PMID: 26750258]
HEK293	IC₅₀	12.1 μM Compound: 1	Antagonist activity at rat TRPA1 expressed in HEK293 cells assessed as inhibition of allyl isothiocyanate-induced increase of intracellular calcium level Antagonist activity at rat TRPA1 expressed in HEK293 cells assessed as inhibition of allyl isothiocyanate-induced increase of intracellular calcium level	[PMID: 26750258]
J774	IC₅₀	16.6 μM Compound: 1	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 29726680]
J774	IC₅₀	32.1 μM Compound: 1	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced IL6 expression after 24 hrs by ELISA Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced IL6 expression after 24 hrs by ELISA	[PMID: 29726680]
J774	IC₅₀	38.7 μM Compound: 1	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced MCP1 expression after 24 hrs by ELISA Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced MCP1 expression after 24 hrs by ELISA	[PMID: 29726680]
RAW264.7	IC₅₀	10.6 μM Compound: Pinosylvin	inhibitory concentration against PGE-2 production in LPS-stimulated RAW264.7 cells inhibitory concentration against PGE-2 production in LPS-stimulated RAW264.7 cells	[PMID: 15501064]
U-87MG ATCC	IC₅₀	88.96 μM Compound: Pinosylvin	Antitumor activity against human U87 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay Antitumor activity against human U87 cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay	[PMID: 38493731]

In Vitro

Pinosylvin (0-100 μM; 24 hours) is cytotoxic to THP‐1 and U937 cells, exhibits an IC₅₀ value of 20-30 μM in leukemia cells, the maximal cytotoxic effect occurred at 100 μM following incubation for 24?hr^[3].
Pinosylvin (0-100 μM; 24 hours) enhances the number of annexin V⁺?and PI⁺?cells in the U937 population at 50 μM, increases annexin V⁺?and PI⁺ cells in the THP‐1 population at 100 μM^[3].
Pinosylvin (0-100 μM; 24 hours) promotes autophagy in leukemia cells by enhancing the level of LC3‐II and p62/SQSTM1 degradation in leukemia cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

Leukemia cells
Concentration:

0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM
Incubation Time:

24 hours
Result:

Was cytotoxic to leukemia cells at high concentrations.

Apoptosis Analysis^[3]

Cell Line:

U937 and THP‐1 cells
Concentration:

0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM
Incubation Time:

24 hours
Result:

Induced apoptosis cell number in U937 and THP‐1 cells.

Western Blot Analysis^[3]

Cell Line:

U937 and THP‐1 cells
Concentration:

0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM
Incubation Time:

24 hours
Result:

Induced autophagy in leukemia cells.

In Vivo

Pinosylvin (intravenous injection; 10 mg/kg) yields the plasma AUC, urine t , CL and Vd values of 5.23 ± 1.20 mgh mL^-1, 13.13 ± 2.05 h, 1.84 ± 0.44 Lh^-1kg^-1 and 2.29 Lkg^-1, respectively in male Sprague-Dawley rats, in a PK study^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 22139-77-1
Appearance Solid
Molecular Weight 212.24
Formel C₁₄H₁₂O₂
Color White to off-white
SMILES

OC1=CC(/C=C/C2=CC=CC=C2)=CC(O)=C1
Structure Classification
- Phenols
- Polyphenols
- Stilbenes
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (471.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.7116 mL

23.5582 mL

47.1165 mL

5 mM

0.9423 mL

4.7116 mL

9.4233 mL

10 mM

0.4712 mL

2.3558 mL

4.7116 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lee SK, et al. Antibacterial and antifungal activity of pinosylvin, a constituent of pine.Fitoterapia. 2005 Mar;76(2):258-60. [Content Brief]

[2]. Roupe KA, et al. Pharmacokinetics of selected stilbenes: rhapontigenin, piceatannol and pinosylvin in rats.J Pharm Pharmacol. 2006 Nov;58(11):1443-50. [Content Brief]

[3]. Song J, et al. Pinosylvin enhances leukemia cell death via down-regulation of AMPKα expression. Phytother Res. 2018 Oct;32(10):2097-2104. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.7116 mL	23.5582 mL	47.1165 mL	117.7912 mL
	5 mM	0.9423 mL	4.7116 mL	9.4233 mL	23.5582 mL
	10 mM	0.4712 mL	2.3558 mL	4.7116 mL	11.7791 mL
	15 mM	0.3141 mL	1.5705 mL	3.1411 mL	7.8527 mL
	20 mM	0.2356 mL	1.1779 mL	2.3558 mL	5.8896 mL
	25 mM	0.1885 mL	0.9423 mL	1.8847 mL	4.7116 mL
	30 mM	0.1571 mL	0.7853 mL	1.5705 mL	3.9264 mL
	40 mM	0.1178 mL	0.5890 mL	1.1779 mL	2.9448 mL
	50 mM	0.0942 mL	0.4712 mL	0.9423 mL	2.3558 mL
	60 mM	0.0785 mL	0.3926 mL	0.7853 mL	1.9632 mL
	80 mM	0.0589 mL	0.2945 mL	0.5890 mL	1.4724 mL
	100 mM	0.0471 mL	0.2356 mL	0.4712 mL	1.1779 mL

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Pinosylvin

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Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Pinosylvin Related Classifications

HY-N2387 Related Classifications