N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride
Based on 1 Customer Validation
N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride (UAA crosslinker 1 hydrochloride) is a click chemistry-reactive amino acid derivative. N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride mediates the generation of antibody-drug conjugates (ADCs). N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride is used for site-specific incorporation into recombinant proteins, or for synthesizing chemical probes and tools for biological research.
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- Reinheit : 98.0%
- CAS. Nr.: 1994331-17-7
- Formel: C9H18ClN5O4
- Molecular Weight:295.72
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
![N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride Chemical Structure](https://file.medchemexpress.com/product_pic/hy-111434a.gif "Click to View N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride Chemical Structure")
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride promotes the generation of antibody-drug conjugates (ADCs)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 1994331-17-7
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Appearance Solid
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Molecular Weight 295.72
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Formel C9H18ClN5O4
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Color Off-white to light yellow
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SMILES
N[C@@H](CCCCNC(OCCN=[N+]=[N-])=O)C(O)=O.[H]Cl
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Synonyms
UAA crosslinker 1 hydrochloride
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 125 mg/mL (422.70 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (338.16 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (7.03 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
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Data Sheet (269 KB)
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SDS (406 KB)
- English - EN (406 KB)
- Français - FR (406 KB)
- Deutsch - DE (406 KB)
- Norwegian - NO (406 KB)
- Español - ES (406 KB)
- Swedish - SV (406 KB)
- Italian - IT (406 KB)
- Korean - KR (406 KB)
- Portuguese - PT (406 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.3816 mL | 16.9079 mL | 33.8158 mL | 84.5394 mL |
| 5 mM | 0.6763 mL | 3.3816 mL | 6.7632 mL | 16.9079 mL | |
| 10 mM | 0.3382 mL | 1.6908 mL | 3.3816 mL | 8.4539 mL | |
| 15 mM | 0.2254 mL | 1.1272 mL | 2.2544 mL | 5.6360 mL | |
| 20 mM | 0.1691 mL | 0.8454 mL | 1.6908 mL | 4.2270 mL | |
| 25 mM | 0.1353 mL | 0.6763 mL | 1.3526 mL | 3.3816 mL | |
| 30 mM | 0.1127 mL | 0.5636 mL | 1.1272 mL | 2.8180 mL | |
| 40 mM | 0.0845 mL | 0.4227 mL | 0.8454 mL | 2.1135 mL | |
| 50 mM | 0.0676 mL | 0.3382 mL | 0.6763 mL | 1.6908 mL | |
| 60 mM | 0.0564 mL | 0.2818 mL | 0.5636 mL | 1.4090 mL | |
| 80 mM | 0.0423 mL | 0.2113 mL | 0.4227 mL | 1.0567 mL | |
| 100 mM | 0.0338 mL | 0.1691 mL | 0.3382 mL | 0.8454 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N6-[(2-Azidoethoxy)carbonyl]-L-lysine hydrochloride Related Classifications
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