URAT1-IN-14
URAT1-IN-14 is a potent and orally active Urate transporter 1 (URAT1) inhibitor. URAT1-IN-14 inhibits the human URAT1 in HEK293 cells with an IC50 of 0.72 μM. URAT1-IN-14 exhibits low cytotoxic in Hep-G2 cells. URAT1-IN-14 shows urate-lowering effect in hyperuricemia mouse models. URAT1-IN-14 can be used for the study of hyperuricemia and gout.
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- CAS. Nr.: 2516232-89-4
- Formel: C19H19NO3S
- Molecular Weight:341.42
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
URAT1-IN-14 (Compound 7) (IC50 = 326.5 μM) was less cytotoxic than Benzbromarone (HY-B1135) (IC50 = 67.21 μM) in Hep-G2 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sub-HUA model induced by uric acid and OP in mice[1]
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Dosage:25, 50 and 100 mg/kg
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Administration:Oral administration (p.o.), single dose
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Result:Significantly lowers the blood uric acid level within 24 hours at the dosage of 100 mg/kg.
Decreased the AUC0-24h values treated groups in a dose-dependent manner 6%, 17%, 34%, at the doses of 25, 50 and 100 mg/kg, respectively.
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Animal Model:Ch-HUA model induced by purine-rich yeast extraction and OP in mice[1]
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Dosage:25, 50 and 100 mg/kg
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Administration:Oral administration (p.o.), for 9 days
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Result:Drastically decreased blood uric acid level at the dosage of 50 mg/kg.
Chemical Information
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CAS. Nr. 2516232-89-4
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Molecular Weight 341.42
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Formel C19H19NO3S
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SMILES
O=C(C1(SC2=CC=CC=C2N3CCOC4=CC=CC=C34)CCC1)O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
URAT1-IN-14 Related Classifications
HY-175309 Related Classifications
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