Methoxy-PMS
Based on 8 publication(s) in Google Scholar
Methoxy-PMS (1-Methoxy PMS), an active oxygen formation inducer, is stable electron-transport mediator between NAD(P)H and tetrazolium dyes.
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- Pureté: 99.32%
- CAS No.: 65162-13-2
- Formule: C15H16N2O5S
- Masse moléculaire:336.36
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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NF-κB Signaling Compound Library
HY-L014
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Anti-Aging Compound Library
HY-L034
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Lactylation Compound Library
HY-L249
Publications Citing Use of MedChemExpress (MCE) Methoxy-PMS
More- Talanta. 2025 Nov 25:300:129159. [Abstract]
- Biomacromolecules. 2026 Feb 9;27(2):1317-1336. [Abstract]
- Chem Biol Interact. 2024 Jun 21:398:111113. [Abstract]
- Cells. 2026 May 14;15(10):898. [Abstract]
- Mitochondrion. 2026 Mar:87:102112. [Abstract]
- J Pharm Biomed Anal. 2026 Oct 15:279:117580. [Abstract]
- PLoS One. 2022 Jul 22;17(7):e0271818. [Abstract]
- protocols.io. 2023 jan 31.
Activité biologique
Methoxy-PMS has no cytotoxicity in the cell culture media. Methoxy-PMS receives an electron from NADH or NADPH at the membrane or inside of the cell and passes the electron to the WST-8 that is around the outer cell membrane[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 65162-13-2
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Appearance Solid
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Masse moléculaire 336.36
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Formule C15H16N2O5S
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Color Brown to reddish brown
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SMILES
C[N+]1=C2C=CC=CC2=NC3=C1C=CC=C3OC.O=S(OC)([O-])=O
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Synonyms
1-Methoxy PMS; 1-Methoxyphenazine methosulfate
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)
Publications (8)
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Journal Impact Factor
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Most Recent
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Talanta
Colorimetric determination of the plant toxin β-cyano-l-alanine using a multistep enzyme reaction. [Abstract]2025 Nov 25:300:129159. PMID: 41338014 -
Biomacromolecules
Tyrosinase Cross-Linked PEG Hydrogels with DAT and DATT as Artificial Substrates: Design, Structure, and Functions. [Abstract]2026 Feb 9;27(2):1317-1336. PMID: 41586814 -
Chem Biol Interact
N-desmethyldauricine from Menispermum dauricum DC suppresses triple-negative breast cancer growth in 2D and 3D models by downregulating the NF-κB signaling pathway. [Abstract]2024 Jun 21:398:111113. PMID: 38908813 -
Cells
2026 May 14;15(10):898. PMID: 42193905 -
Mitochondrion
Sensitivity of primary mitochondrial disease fibroblasts to ferroptosis: The role of intracellular iron. [Abstract]2026 Mar:87:102112. PMID: 41581630 -
J Pharm Biomed Anal
Development of rapid diagnostic kit against sepsis: Barcode-based semi-quantitative measurement of C-reactive protein and lactate. [Abstract]2026 Oct 15:279:117580. PMID: 42190402 -
PLoS One
L-lactate as an indicator for cellular metabolic status: An easy and cost-effective colorimetric L-lactate assay. [Abstract]2022 Jul 22;17(7):e0271818. PMID: 35867690 -
Solvant et solubilité
DMSO : 62.5 mg/mL (185.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 6.67 mg/mL (19.83 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.25 mg/mL (6.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.25 mg/mL (6.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 9.09 mg/mL (27.02 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. De Niz M et al. Tools for mass screening of G6PD deficiency: validation of the WST8/1-methoxy-PMS enzymatic assay in Uganda. Malar J. 2013 Jun 19;12:210. [Content Brief]
[2]. Arakawa H et al. Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol-microperoxidase in the presence of 1-methoxy PMS. Luminescence. 2007 May-Jun;22(3):245-50. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.9730 mL | 14.8650 mL | 29.7301 mL | 74.3251 mL |
| 5 mM | 0.5946 mL | 2.9730 mL | 5.9460 mL | 14.8650 mL | |
| 10 mM | 0.2973 mL | 1.4865 mL | 2.9730 mL | 7.4325 mL | |
| 15 mM | 0.1982 mL | 0.9910 mL | 1.9820 mL | 4.9550 mL | |
| DMSO | 20 mM | 0.1487 mL | 0.7433 mL | 1.4865 mL | 3.7163 mL |
| 25 mM | 0.1189 mL | 0.5946 mL | 1.1892 mL | 2.9730 mL | |
| 30 mM | 0.0991 mL | 0.4955 mL | 0.9910 mL | 2.4775 mL | |
| 40 mM | 0.0743 mL | 0.3716 mL | 0.7433 mL | 1.8581 mL | |
| 50 mM | 0.0595 mL | 0.2973 mL | 0.5946 mL | 1.4865 mL | |
| 60 mM | 0.0496 mL | 0.2478 mL | 0.4955 mL | 1.2388 mL | |
| 80 mM | 0.0372 mL | 0.1858 mL | 0.3716 mL | 0.9291 mL | |
| 100 mM | 0.0297 mL | 0.1487 mL | 0.2973 mL | 0.7433 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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