ATTO 647

ATTO 647 (0.3 mg; 2 h) can covalently label the purified WGA, with an average of 1.5 ATTO 647 fluorescent groups binding to each WGA molecule^[1].
ATTO 647 (100 nM) in the Atto 647-WGA conjugate will exhibit single-step or double-step photobleaching when bound to the hyaluronic acid-coated glass cover slip, which is consistent with the situation where each conjugate contains one or two fluorescent groups^[1].

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
Application of ATTO 647 in the Stably Expressing Group Protein H2B-GFP Human HeLa Cells for Staining^[2][3]:
1. Cultivate the cells until 50-80% confluence, fix with 3.7% formaldehyde.
2. Triton X-100 0.5% permeabilization, BSA/PBST 2% blocking.
3. Incubate with ATTO 647N conjugated antibody to label the intranuclear group protein H2B-GFP.
4. Seal the sections with the following media: PBS, Vectashield (VS), Ibidi-MM, ROXS (AA/MV) (1 mM ascorbic acid + 1 mM methylene blue + enzymatic deoxygenation system), ROXS (TX/TQ) (1 mM Trolox + UV pre-generated Trolox - quinone + enzymatic deoxygenation system).
5. Observe the results under a 635 nm laser.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.