CPM
Based on 1 Customer Validation
CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye (excitation/emission maxima of 387/463 nm).
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- Pureté: 98.87%
- CAS No.: 76877-33-3
- Formule: C24H22N2O4
- Masse moléculaire:402.44
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
1. Preparation of CPM working solution
1.1 Preparation of stock solution
Freshly prepare CPM DMSO solution with a concentration of 4.0 mg/mL.
1.2 Preparation of CPM working solution
Dilute it 40 times in assay buffer to prepare working solution.
Note: Keep it on ice during the experiment.
2. Formal experiment
2.1 CPM reactivity experiment
a. Mix CPM working solution (30 μL) with 570 μL assay buffer.
b. Monitor the fluorescence signal as the temperature increases (excitation wavelength 387 nm, emission wavelength 463 nm).
c. Analyze the value of melting temperature (Tm) according to the Bolzmann equation.
2.2 Covalent interaction evaluation experiment
a. Mix 30 μL of CPM working solution with 570 μL of assay buffer (buffer pH 8.0) containing 50 mM glycine.
b. Incubate at 80 °C for 30 min in the dark.
c. Cool the sample to 20 °C and add lysozyme to a final concentration of 4 μM.
d. Monitor the change in fluorescence intensity after heating.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 76877-33-3
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Appearance Solid
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Masse moléculaire 402.44
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Formule C24H22N2O4
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Color Light yellow to yellow
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SMILES
O=C(C=C1)N(C2=CC=C(C3=C(C)C4=CC=C(N(CC)CC)C=C4OC3=O)C=C2)C1=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
DMSO : 25 mg/mL (62.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Grossman SH. Resonance energy transfer between the active sites of myocardial-type creatine kinase (isozyme MB). Biochemistry. 1983 Nov 8;22(23):5369-75. [Content Brief]
[2]. Wang Z, et al. Cysteine residue is not essential for CPM protein thermal-stability assay. Anal Bioanal Chem. 2015 May;407(13):3683-91. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4848 mL | 12.4242 mL | 24.8484 mL | 62.1211 mL |
| 5 mM | 0.4970 mL | 2.4848 mL | 4.9697 mL | 12.4242 mL | |
| 10 mM | 0.2485 mL | 1.2424 mL | 2.4848 mL | 6.2121 mL | |
| 15 mM | 0.1657 mL | 0.8283 mL | 1.6566 mL | 4.1414 mL | |
| 20 mM | 0.1242 mL | 0.6212 mL | 1.2424 mL | 3.1061 mL | |
| 25 mM | 0.0994 mL | 0.4970 mL | 0.9939 mL | 2.4848 mL | |
| 30 mM | 0.0828 mL | 0.4141 mL | 0.8283 mL | 2.0707 mL | |
| 40 mM | 0.0621 mL | 0.3106 mL | 0.6212 mL | 1.5530 mL | |
| 50 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2424 mL | |
| 60 mM | 0.0414 mL | 0.2071 mL | 0.4141 mL | 1.0354 mL |
CPM Related Classifications
HY-D0069 Related Classifications
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