M50054
Based on 1 Customer Validation
M50054 is a potent inhibitor of apoptosis. M50054 inhibits Etoposide-induced caspase-3 activation of U937 cells with an IC50 of 79 μg/mL. M50054 does not directly inhibit the enzymatic activity of caspase-3. M50054 can be used for the research anti-Fas-antibody-induced hepatitis and chemotherapy-induced alopecia.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.44%
- CAS No.: 54135-60-3
- Formule: C13H16O4
- Masse moléculaire:236.26
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
M50054 inhibits the caspase-3 activation by Etoposide in a dose-dependent manner. The IC50 value of M50054 against Etoposide-induced caspase-3 activation of U937 cells is 79 μg/mL. However, M50054 does not directly inhibit the enzymatic activity of caspase-3 at all concentrations up to 1000 μg/mL[1].
M50054 (1-300 μg/mL) inhibits soluble human Fas ligand-induced cell death of human Fas-expressing WC8 cells in a dose-dependent manner, and the IC50 of 67 μg/mL[1].
M50054 (30-300 μg/mL) inhibits the death of U937 cells induced by etoposide (10 μg/mL) in a dose-dependent manner, and the IC50 value of M50054 against Etoposide-induced cell death of U937 cells is 130 μg/mL. The IC50 value of M50054 against etoposide-induced DNA fragmentation of U937 cells was 54 μg/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human Fas-expressing WC8 cells
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Concentration:1, 3, 10, 30, 100, 300 μg/mL
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Incubation Time:Pre-incubated for 30 min
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Result:Inhibited soluble human Fas ligand-induced cell death of human Fas-expressing WC8 cells in a dose-dependent manner, and the IC50 of 67 μg/mL.
M50054 (topical treatment; given daily beginning on day 5 after birth and ending on day 14) significantly improves alopecia (hair loss) symptoms[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c mice[1]
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Dosage:10, 30, 100 or 300 mg/kg
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Administration:Orally administered in a volume of 10 mL/kg
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Result:Inhibited the elevation of alanine aminotransferase and aspartate aminotransferase in a dose-dependent manner.
Significantly improved these parameters at doses of 100 mg/kg and 300 mg/kg.
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Animal Model:Five-day-old SD rats[1]
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Dosage:
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Administration:Topically applied to the head and back of 5-day-old rats once daily for 10 days
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Result:Alopecia scores significantly decreased throughout the entire observation period compared with the control group.
Chemical Information
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CAS No. 54135-60-3
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Appearance Solid
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Masse moléculaire 236.26
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Formule C13H16O4
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Color Off-white to light yellow
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SMILES
O=C1C(CC(C(CCC2)=O)C2=O)C(CCC1)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : ≥ 100 mg/mL (423.26 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.2326 mL | 21.1631 mL | 42.3263 mL | 105.8156 mL |
| 5 mM | 0.8465 mL | 4.2326 mL | 8.4653 mL | 21.1631 mL | |
| 10 mM | 0.4233 mL | 2.1163 mL | 4.2326 mL | 10.5816 mL | |
| 15 mM | 0.2822 mL | 1.4109 mL | 2.8218 mL | 7.0544 mL | |
| 20 mM | 0.2116 mL | 1.0582 mL | 2.1163 mL | 5.2908 mL | |
| 25 mM | 0.1693 mL | 0.8465 mL | 1.6931 mL | 4.2326 mL | |
| 30 mM | 0.1411 mL | 0.7054 mL | 1.4109 mL | 3.5272 mL | |
| 40 mM | 0.1058 mL | 0.5291 mL | 1.0582 mL | 2.6454 mL | |
| 50 mM | 0.0847 mL | 0.4233 mL | 0.8465 mL | 2.1163 mL | |
| 60 mM | 0.0705 mL | 0.3527 mL | 0.7054 mL | 1.7636 mL | |
| 80 mM | 0.0529 mL | 0.2645 mL | 0.5291 mL | 1.3227 mL | |
| 100 mM | 0.0423 mL | 0.2116 mL | 0.4233 mL | 1.0582 mL |
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