2. Antibody-drug Conjugate/ADC Related
  3. Drug-Linker Conjugates for ADC
  4. CL2A-SN-38

CL2A-SN-38 is a agent-linker conjugate composed of a potent a DNA Topoisomerase I inhibitor SN-38 and a linker CL2A to make antibody agent conjugate (ADC). CL2A-SN-38 provides significant and specific antitumor effects against a range of human solid tumor types. CL2A-SN-38 uses hydrolyzable linker to deliver active agents within tumor cells and in the tumor microenvironment, resulting in bystander effects.

For research use only. We do not sell to patients.

CAS No. : 1279680-68-0

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Customer Review

Based on 5 publication(s) in Google Scholar

CL2A-SN-38 Related Antibodies

Top Publications Citing Use of Products
Bio/Physico-chemical Assay
Cell Proliferation/Viability Assay
In Vivo Efficacy Study

    CL2A-SN-38 purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2025 Jul 25;17(8):967.  [Abstract]

    412a ADC with dual MMAF and SN38 payloads improved potency and efficacy over single-payload 412a ADCs. A panel of 8 cell lines consisting of gastric, pancreatic, small cell lung, and triple negative breast cancer cell lines was treated with a molar (M) dilution series of 412a-MMAF, 412a-SN38, or 412a-druxtecan (Dxd), 412a-MMAF+SN38 dual-payload ADC, and unconjugated 412a for 72 h. Cell viability at each concentration of ADC was determined using a luminescent readout of ATP levels in relative light units (RLU). Data were shown from two representative cancer cell lines, BxPC-3 and MKN45 cells. Curves were fitted using a nonlinear regression.

    CL2A-SN-38 purchased from MedChemExpress. Usage Cited in: Pharmaceutics. 2025 Jul 25;17(8):967.  [Abstract]

    Half-maximal inhibitory concentration (IC50) values were generated from cell viability curves to determine the potency of each test article. Each data point represented a different cell line. Groups without 8 data points had cell lines that were not responsive enough to the ADC to generate an IC50 value.

    CL2A-SN-38 purchased from MedChemExpress. Usage Cited in: AAPS J. 2022 May 27;24(4):70.  [Abstract]

    SY02-SN-38 was the antibody-drug conjugate (ADC) prepared by direct conjugation of the antibody SY02 with CL2A-SN-38. In vitro comparison of cytotoxicity of SY02-ADCs in Trop-2-expressing CFPAC-1 cells and MDA-MB-468 cells was conducted.

    CL2A-SN-38 purchased from MedChemExpress. Usage Cited in: AAPS J. 2022 May 27;24(4):70.  [Abstract]

    SY02-SN-38 was the antibody-drug conjugate (ADC) prepared by direct conjugation of the antibody SY02 with CL2A-SN-38. The in vivo antitumor activity of SY02-ADCs in CFPAC-1 xenograft mouse models was evaluated.

    CL2A-SN-38 purchased from MedChemExpress. Usage Cited in: AAPS J. 2022 May 27;24(4):70.  [Abstract]

    SY02-SN-38 was the antibody-drug conjugate (ADC) prepared by direct conjugation of the antibody SY02 with CL2A-SN-38. Plasma concentration–time curves of total antibody and free payloads were presented.

    View All Drug-Linker Conjugates for ADC Isoform Specific Products:

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    Auristatin Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents Dual payloads

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    • Purity & Documentation

    • References

    • Customer Review

    Description

    CL2A-SN-38 is a agent-linker conjugate composed of a potent a DNA Topoisomerase I inhibitor SN-38 and a linker CL2A to make antibody agent conjugate (ADC). CL2A-SN-38 provides significant and specific antitumor effects against a range of human solid tumor types. CL2A-SN-38 uses hydrolyzable linker to deliver active agents within tumor cells and in the tumor microenvironment, resulting in bystander effects[1].

    IC50 & Target

    Camptothecins

     

    In Vivo

    CL2A-SN-38 coupled to the anti-Trop-2 humanized antibody hRS7 (intraperitoneal injection, 0.2 or 0.4 mg/kg, twice weekly, 4 weeks) can both significantly inhibit tumor growth in mice with human colon (COLO 205) or pancreatic (Capan-1) tumour xenografts[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    1480.61

    Formula

    C73H97N11O22
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(O[C@](C1=C(CO2)C(N3CC4=C(CC)C5=CC(O)=CC=C5N=C4C3=C1)=O)(CC)C2=O)OCC6=CC=C(NC([C@H](CCCCN)NC(COCC(NCCOCCOCCOCCOCCOCCOCCOCCOCCN7N=NC(CNC(C8CCC(CN9C(C=CC9=O)=O)CC8)=O)=C7)=O)=O)=O)C=C6
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (67.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.6754 mL 3.3770 mL 6.7540 mL
    5 mM 0.1351 mL 0.6754 mL 1.3508 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.08 mg/mL (1.40 mM); Clear solution; Need ultrasonic

      This protocol yields a clear solution of 2.08 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (1.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.97%

    SDS (419 KB)

    COA (252 KB) HNMR (228 KB) RP-HPLC (235 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.6754 mL 3.3770 mL 6.7540 mL 16.8849 mL
    5 mM 0.1351 mL 0.6754 mL 1.3508 mL 3.3770 mL
    10 mM 0.0675 mL 0.3377 mL 0.6754 mL 1.6885 mL
    15 mM 0.0450 mL 0.2251 mL 0.4503 mL 1.1257 mL
    20 mM 0.0338 mL 0.1688 mL 0.3377 mL 0.8442 mL
    25 mM 0.0270 mL 0.1351 mL 0.2702 mL 0.6754 mL
    30 mM 0.0225 mL 0.1126 mL 0.2251 mL 0.5628 mL
    40 mM 0.0169 mL 0.0844 mL 0.1688 mL 0.4221 mL
    50 mM 0.0135 mL 0.0675 mL 0.1351 mL 0.3377 mL
    60 mM 0.0113 mL 0.0563 mL 0.1126 mL 0.2814 mL
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    CL2A-SN-38 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CL2A-SN-381279680-68-0Drug-Linker Conjugates for ADCDNA Topoisomerase ISN-38CL2AADCantitumorbystander effectInhibitorinhibitorinhibit

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