Cat. No.: HY-N2535

Coniferaldehyde

(Synonyms: 4-Hydroxy-3-methoxycinnamaldehyde)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Coniferaldehyde (4-Hydroxy-3-methoxycinnamaldehyde) is an effective inducer of heme oxygenase-1 (HO-1). Coniferaldehyde inhibits LPS-induced apoptosis through the PKCα/β II/Nrf-2/HO-1 dependent pathway in RAW264.7 macrophage cells. Coniferaldehyde has antioxidant and anti-inflammatory activities.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 458-36-6
Formula: C₁₀H₁₀O₃
Molecular Weight:178.18

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Coniferaldehyde Chemical Structure

CAS No.: 458-36-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	1.7 μM Compound: 10	Growth inhibition of human A549 cells by Sulforhodamine B assay Growth inhibition of human A549 cells by Sulforhodamine B assay	[PMID: 21106458]
A549	IC₅₀	21.18 μM Compound: 19	Anticancer activity against human A549 cells by SRB assay Anticancer activity against human A549 cells by SRB assay	[PMID: 21420296]
A549	IC₅₀	59 μM Compound: 30	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
B16-4A5	IC₅₀	15.3 μM Compound: 15	Antimelanogenic activity in theophylline-stimulated mouse B16-4A5 cells assessed as inhibition of melanogenesis after 72 hrs relative to control Antimelanogenic activity in theophylline-stimulated mouse B16-4A5 cells assessed as inhibition of melanogenesis after 72 hrs relative to control	[PMID: 27756508]
HeLa	GI₅₀	61.8 μM Compound: 10	Growth inhibition of human HeLa cells by Sulforhodamine B assay Growth inhibition of human HeLa cells by Sulforhodamine B assay	[PMID: 21106458]
HeLa	EC₅₀	9.03 μM Compound: 5	Induction of cell surface translocation of MR1 in human HeLa overexpressing MR1 assessed as increase in MR1 cell surface levels incubated for 8 hrs followed by labeling with PE-conjugated anti-hMR1 antibody by flow cytometry analysis Induction of cell surface translocation of MR1 in human HeLa overexpressing MR1 assessed as increase in MR1 cell surface levels incubated for 8 hrs followed by labeling with PE-conjugated anti-hMR1 antibody by flow cytometry analysis	[PMID: 37638616]
HT-1080	ED₅₀	4.05 μg/mL Compound: 9	Cytotoxicity against human HT1080 cells after 24 hrs by MTT assay Cytotoxicity against human HT1080 cells after 24 hrs by MTT assay	[PMID: 9677271]
LoVo	IC₅₀	23 μM Compound: 30	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
MCF7	GI₅₀	39.5 μM Compound: 10	Growth inhibition of human MCF7 cells by Sulforhodamine B assay Growth inhibition of human MCF7 cells by Sulforhodamine B assay	[PMID: 21106458]
PC-3	IC₅₀	35 μM Compound: 30	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
RAW264.7	IC₅₀	>0.3 mM Compound: 30	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs	[PMID: 18986199]
RAW264.7	IC₅₀	>0.3 μM/mL Compound: 30	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs	[PMID: 18986199]
SK-MEL-2	IC₅₀	>30 μM Compound: 19	Anticancer activity against human SK-MEL-2 cells by SRB assay Anticancer activity against human SK-MEL-2 cells by SRB assay	[PMID: 21420296]
SK-MEL-28	IC₅₀	33 μM Compound: 30	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
SK-N-SH	GI₅₀	10.6 μM Compound: 10	Growth inhibition of human SK-N-SH cells by Sulforhodamine B assay Growth inhibition of human SK-N-SH cells by Sulforhodamine B assay	[PMID: 21106458]
SK-OV-3	IC₅₀	>30 μM Compound: 19	Anticancer activity against human SKOV3 cells by SRB assay Anticancer activity against human SKOV3 cells by SRB assay	[PMID: 21420296]
U-373MG ATCC	IC₅₀	28 μM Compound: 30	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]
XF498	IC₅₀	>30 μM Compound: 19	Anticancer activity against human XF498 cells by SRB assay Anticancer activity against human XF498 cells by SRB assay	[PMID: 21420296]

In Vitro

Coniferaldehyde (0.1-5 μM; Pretreatment with 1 h; and then treated with 24 h) exerts cytoprotective function against LPS-induced cell death and dramatically inhibits LPS-induced NO production as assessed by the Griess reaction in a dose dependent manner^[1].
Coniferaldehyde (0.5-5 μM; 4-24 h) dramatically increases the Nrf-2 nuclear translocation and HO-1 expression. Furthermore, Coniferaldehyde specifically enhances the phosphorylation of PKCα/PKCβ II^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Raw264.7 cells inudced with LPS
Concentration:

0.1 μM, 0.5 μM, 1 μM, 2 μM, 5 μM
Incubation Time:

Pretreatment with 1 h, and then treated with 24 h
Result:

Inhibited LPS-induced NO production and cell death.

Western Blot Analysis^[1]

Cell Line:

Raw264.7 cells
Concentration:

0.5 μM, 1 μM, 2 μM, 5 μM
Incubation Time:

4 h, 8 h, 12 h, 24 h
Result:

Increased HO-1 protein level in a dose- and time-dependent manner.

In Vivo

Coniferaldehyde (0.05 mmol kg/day; i.p; for 6 weeks) activates the Nrf2 signaling pathway in primary chondrocytes and articular cartilage from the knee joints. And Coniferaldehyde alleviates cartilage destruction in OA mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

B6 male mice (26 g; 8-10 weeks old), surgical-induced osteoarthritis (OA)^[2]
Dosage:

0.05 mmol kg/day (approx. 8.9 mg/kg)
Administration:

i.p; for 6 weeks
Result:

Evidently alleviated the medial meniscus cartilage damage in mice subjected to the destabilization.

Chemical Information

CAS No. 458-36-6
Appearance Solid
Molecular Weight 178.18
Formula C₁₀H₁₀O₃
Color Light yellow to brown
SMILES

O=C/C=C/C1=CC=C(O)C(OC)=C1
Synonyms

4-Hydroxy-3-methoxycinnamaldehyde
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Monophenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

bioRxiv

2024 May 21.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (561.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.6123 mL

28.0615 mL

56.1230 mL

5 mM

1.1225 mL

5.6123 mL

11.2246 mL

10 mM

0.5612 mL

2.8062 mL

5.6123 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1 mg/mL (5.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1 mg/mL (5.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (5.61 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kim KM, et al. Coniferaldehyde inhibits LPS-induced apoptosis through the PKC α/β II/Nrf-2/HO-1 dependent pathway in RAW264.7 macrophage cells. Environ Toxicol Pharmacol. 2016 Dec;48:85-93. [Content Brief]

[2]. Dawei Cai, et al. Coniferaldehyde prevents articular cartilage destruction in a murine model via Nrf2/HO‑1 pathway. Mol Med Rep. 2021 Mar;23(3):224. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.6123 mL	28.0615 mL	56.1230 mL	140.3076 mL
	5 mM	1.1225 mL	5.6123 mL	11.2246 mL	28.0615 mL
	10 mM	0.5612 mL	2.8062 mL	5.6123 mL	14.0308 mL
	15 mM	0.3742 mL	1.8708 mL	3.7415 mL	9.3538 mL
	20 mM	0.2806 mL	1.4031 mL	2.8062 mL	7.0154 mL
	25 mM	0.2245 mL	1.1225 mL	2.2449 mL	5.6123 mL
	30 mM	0.1871 mL	0.9354 mL	1.8708 mL	4.6769 mL
	40 mM	0.1403 mL	0.7015 mL	1.4031 mL	3.5077 mL
	50 mM	0.1122 mL	0.5612 mL	1.1225 mL	2.8062 mL
	60 mM	0.0935 mL	0.4677 mL	0.9354 mL	2.3385 mL
	80 mM	0.0702 mL	0.3508 mL	0.7015 mL	1.7538 mL
	100 mM	0.0561 mL	0.2806 mL	0.5612 mL	1.4031 mL

Keywords

Coniferaldehyde

1 Cited Publications

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Coniferaldehyde

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Publications Citing Use of MedChemExpress (MCE) Coniferaldehyde

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Coniferaldehyde Related Classifications

HY-N2535 Related Classifications