2. Cytoskeleton
  3. Cadherin
  4. CQY684

CQY684  (Synonyms: PCA062 antibody)

Cat. No.: HY-P991342 Purity: 97.60%
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CQY684 (PCA062 antibody) is a monoclonal antibody targeting CDH3/P-cadherin, which locks P-cadherin in an X-dimer conformation and enhances the stability of this adhesion structure. CQY684 induces P-cadherin phosphorylation and promotes its dissociation from the cytoplasmic region of P-cadherin. As an endocytosis inducer and lysosome-targeting agent, CQY684 facilitates the internalization of the P-cadherin-CQY684 complex and improves the turnover efficiency of P-cadherin. CQY684 serves as a platform for the delivery of intracellular anticancer compounds, which is achieved through the targeted lysosomal transport of the antibody-P-cadherin complex. CQY684 is applicable to the research of breast cancer, esophageal cancer, and head and neck cancer.

For research use only. We do not sell to patients.

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CQY684 Related Antibodies

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Description

CQY684 (PCA062 antibody) is a monoclonal antibody targeting CDH3/P-cadherin, which locks P-cadherin in an X-dimer conformation and enhances the stability of this adhesion structure. CQY684 induces P-cadherin phosphorylation and promotes its dissociation from the cytoplasmic region of P-cadherin. As an endocytosis inducer and lysosome-targeting agent, CQY684 facilitates the internalization of the P-cadherin-CQY684 complex and improves the turnover efficiency of P-cadherin. CQY684 serves as a platform for the delivery of intracellular anticancer compounds, which is achieved through the targeted lysosomal transport of the antibody-P-cadherin complex. CQY684 is applicable to the research of breast cancer, esophageal cancer, and head and neck cancer[1].

Isotype

Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity

Human
IC50 & Target

CDH3/P-cadherin
In Vitro

CQY684 (200 nM; overnight) impairs Pcad-mediated intercellular adhesion and aggregation in A431 cells expressing wild-type Pcad[1].
CQY684 (administered when cells reach 70% confluence at 200 nM) promotes the endocytosis of Pcad and its trafficking to lysosomes in A431 cells expressing wild-type Pcad, and reduces the level of Pcad on the cell surface[1].
CQY684 (administered during cell culture) induces phosphorylation of p120 catenin in A431 cells expressing wild-type Pcad, and promotes the dissociation of p120-catenin from Pcad[1].
CQY684 (200 nM; 24+ h) increases the dynamics of Pcad-containing intercellular junctions in A431 cells expressing wild-type Pcad[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CQY684 Related Antibodies

Immunofluorescence[1]

Cell Line: A431 E-cadherin/Pcad double knockout cells rescued with full-length WT Pcad-mCherry
Concentration: 200 nM
Incubation Time: During cell growth to 70% confluence
Result: Increased internalization of Pcad, resulting in significantly higher colocalization with LAMP1 (Pearson's coefficient elevated compared to no-antibody conditions).

Western Blot Analysis[1]

Cell Line: A431 E-cadherin/Pcad double knockout cells rescued with full-length WT Pcad-mCherry
Concentration: 200 nM
Incubation Time: During cell growth to confluence
Result: Reduced the amount of cell surface Pcad compared to no-antibody conditions, though the ratio of surface to total Pcad remained similar across conditions.
Gene ID

1001  [NCBI]

Accession
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

145.94 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[CQY684]
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized Human Cadherin-3 Protein, His Tag can bind CQY684. The EC50 for this effect is 144 ng/mL.
Purity & Documentation
References
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CQY684 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CQY684PCA062 antibodyCQY 684CQY-684CadherinMonoclonal antibody targeting CDH3/P-cadherinbreast canceresophageal cancerhead and neck cancerA431 cellsInhibitorinhibitorinhibit

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