Cy5-PEG3-endo-BCN
Based on 1 Customer Validation
Cy5-PEG3-endo-BCN is a derivative of the Cyanine 5 (Cy5) (HY-D0821) dye bearing 3 PEG units. Cy5-PEG3-endo-BCN contains the lyophilic bidentate macrocyclic ligand endo-BCN, which allows for further synthesis of macrocyclic complexes. In click chemistry, endo-BCN forms stable triazoles with azide-containing molecules under catalyst-free conditions.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 98.40%
- CAS No.: 1881221-50-6
- 화학식: C51H69ClN4O6
- 분자량:869.57
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보관:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
Cy5-PEG3-endo-BCN undergoes metal-free click chemistry with azide-functionalized AE105 (domain A) to generate the target probe (8b) with a purity of >95%[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1881221-50-6
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Appearance Solid
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분자량 869.57
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화학식 C51H69ClN4O6
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Color Purple to purplish red
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SMILES
CC1(C)C2=CC=CC=C2[N+](CCCCCC(NCCOCCOCCOCCNC(OC[C@H]3[C@]4([H])[C@@]3([H])CCC#CCC4)=O)=O)=C1/C=C/C=C/C=C5N(C6=CC=CC=C6C\5(C)C)C.[Cl-]
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
In Vitro:
DMSO : 50 mg/mL (57.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
순도&문서
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1500 mL | 5.7500 mL | 11.4999 mL | 28.7498 mL |
| 5 mM | 0.2300 mL | 1.1500 mL | 2.3000 mL | 5.7500 mL | |
| 10 mM | 0.1150 mL | 0.5750 mL | 1.1500 mL | 2.8750 mL | |
| 15 mM | 0.0767 mL | 0.3833 mL | 0.7667 mL | 1.9167 mL | |
| 20 mM | 0.0575 mL | 0.2875 mL | 0.5750 mL | 1.4375 mL | |
| 25 mM | 0.0460 mL | 0.2300 mL | 0.4600 mL | 1.1500 mL | |
| 30 mM | 0.0383 mL | 0.1917 mL | 0.3833 mL | 0.9583 mL | |
| 40 mM | 0.0287 mL | 0.1437 mL | 0.2875 mL | 0.7187 mL | |
| 50 mM | 0.0230 mL | 0.1150 mL | 0.2300 mL | 0.5750 mL |
Cy5-PEG3-endo-BCN Related Classifications
HY-149618 Related Classifications
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