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IL-21 Protein, Human

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Based on 1 publication(s) in Google Scholar

IL-21 Protein, Human is a class 1 cytokine, which is produced by activated CD4+ T cells. IL-21 Protein stimulation of T cells, B cells and NK cells leads to enhanced proliferation and mature effector function.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • IL-21 Protein, Human Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-21 Protein, Human is a class 1 cytokine, which is produced by activated CD4+ T cells. IL-21 Protein stimulation of T cells, B cells and NK cells leads to enhanced proliferation and mature effector function.

Background

Interleukin-21 (IL-21) is a pleiotropic cytokine that has key functions in both the innate and adaptive immunity and the regulation of autoimmunity. IL-21 has a range of activities likely to potentiate antitumor immunoresponses. IL-21 has a role as a single agent in the treatment of melanoma. IL-21 also has significant activity against various types of cancer in combination with monoclonal antibodies or signaling inhibitors[1]. Recombinant human interleukin-21 (rIL-21), a human recombinant interleukin, with Cetuximab, a monoclonal antibody, targeting the epidermal growth factor receptor[2].

Verified Bioactivity

1.The ED50 is <0.5 ng/mL as measured by Mino cells, corresponding to a specific activity of >2.0 × 106 units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized Human IL-21R at 5 μg/mL (100 μL/well) can bind Human IL-21. The ED50 for this effect is 5.056 ng/mL.
3.Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 6.707 ng/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q9HBE4-1 (Q32-S162)

  • Gene ID
    59067  [NCBI]
  • Molecular Construction
    • N-term
    • IL-21 (Q32-S162)
      Accession # Q9HBE4-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    IL21; Interleukin-21; Interleukin 21; Interleukin; IL-21; CVID11; Za11

  • AA Sequence

    QDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS

  • Predicted Molecular Mass

    15.6 kDa

  • Molecular Weight

    Approximately 15-16 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Davis ID, et al. Clinical and biological efficacy of recombinant human interleukin-21 in patients with stage IV malignant melanoma without prior treatment: a phase IIa trial. Clin Cancer Res. 2009 Mar 15;15(6):2123-9. [Content Brief]

[2]. Steele N, et al. A phase 1 trial of recombinant human IL-21 in combination with cetuximab in patients with metastatic colorectal cancer. Br J Cancer. 2012 Feb 28;106(5):793-8. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 6.707 ng/mL, corresponding to a specific activity is 1.491×105 U/mg.

  • 1/16

    IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    Customer Validation
     IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL). Western blot analysis of the expression levels of IL-21R in HFLS-RA cells. β-actin was used as an internal reference protein.

  • 1/16

    IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    Customer Validation
     IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL). CCK-8 assay was employed to measure cell viability of HFLS-RA cells.

  • 1/16

    IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    Customer Validation
     IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL). Flow cytometry was utilized to analyze the apoptosis rate.

  • 1/16

    IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    Customer Validation
     IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL) . Transmission electron microscopy (TEM) was applied to observe the number of autophagosomes in HFLS-RA cells. Yellow arrow, autophagosome.

  • 1/16

    IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    Customer Validation
     IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL) . Western blot was conducted to detect the protein expression levels of GRP78, Caspase12, cleaved-Caspase12, CHOP.

  • 1/16

    IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    Customer Validation
     IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL) . Western blot was conducted to detect the protein expression levels of LC3II/LC3I, Beclin1, and p62. β-actin was used as an internal reference protein.

  • IL-21 Protein, Human Product Image
    1/16
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • Experimental Validation Results for IL-21 Protein, Human
  • IL-21 Protein, Human Product Image

  • IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL). Western blot analysis of the expression levels of IL-21R in HFLS-RA cells. β-actin was used as an internal reference protein.

  • IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL). CCK-8 assay was employed to measure cell viability of HFLS-RA cells.

  • IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL). Flow cytometry was utilized to analyze the apoptosis rate.

  • IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL) . Transmission electron microscopy (TEM) was applied to observe the number of autophagosomes in HFLS-RA cells. Yellow arrow, autophagosome.

  • IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL) . Western blot was conducted to detect the protein expression levels of GRP78, Caspase12, cleaved-Caspase12, CHOP.

  • IL-21 Protein, Human purchased from MedChemExpress. Usage Cited in: Immunol Invest. 2025 Aug 4:1-24. [Abstract]

    IL-21 Protein, Human (10 ng/mL, 20 ng/mL, and 40 ng/mL) . Western blot was conducted to detect the protein expression levels of LC3II/LC3I, Beclin1, and p62. β-actin was used as an internal reference protein.