Diacylglycerol pyrophosphate

Diacylglycerol pyrophosphate (0-50 μM; 1-30 min) induces potent, dose- and time-dependent PGE₂ production in mouse P388D1 macrophages, reaching a peak of approximately 0.33 ng/10⁶ cells after treatment with 10 μM for 30 min, and a peak of approximately 0.9 ng/10⁶ cells after treatment with 50 μM for 15 min^[1].
Diacylglycerol pyrophosphate (50 μM; 30 min) triggers PGD₂ generation in mouse P388D1 macrophages (this production is dose-dependently inhibited by Suramin (HY-B0879)), mediates arachidonic acid release fully abrogated by group IV cPLA₂ inhibitor MAFP (HY-103334) to confirm cPLA₂ engages in its signaling pathway, and induces phosphorylation of group IV cPLA₂^[1].
Diacylglycerol pyrophosphate (50 μM; 2-30 min) activates p42 and p44 mitogen-activated protein kinases (MAPKs) in mouse P388D1 macrophages in a time-dependent manner, and this activation is inhibited by the mitogen-activated protein kinase kinase inhibitor PD098059 in a dose-dependent manner^[1].
Diacylglycerol pyrophosphate (50 μM; 1-10 min) activates PKC isozymes α, ε and δ in mouse P388D1 macrophages, which is manifested by their rapid translocation to membrane fractions within 1 minute after stimulation, while PKC ζ remains inactive^[1].
Diacylglycerol pyrophosphate (DGPP) stabilizes the bilayer phase of DEPE model membranes, and exhibits a cylindrical effective molecular conformation identical to that of DOPC under physiological pH and salt conditions; the phosphomonoester pK_a values in equimolar DOPC/DOPE multilamellar vesicles are 2.61 and 6.71^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.