DIBAC-GGFG-NH2CH2-Dxd
Based on 1 Customer Validation
DIBAC-GGFG-NH2CH2-Dxd (compound LP4), a Camptothecin (HY-16560) derivative, is a linker-payload of protein-agent conjugates. Dxd (HY-13631D) can be used as a payload for the antibody-coupling drug ADC (DS-8201a).DIBAC-GGFG-NH2CH2-Dxd is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 98.57%
- CAS No.: 2758875-01-1
- Formula: C61H58FN9O12
- Molecular Weight:1128.16
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Drug-Linker Conjugates for ADC Isoforms
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Biological Activity
Description
In Vitro
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2758875-01-1
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Appearance Solid
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Molecular Weight 1128.16
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Formula C61H58FN9O12
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Color Light yellow to yellow
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SMILES
O=C(N1C2=CC=CC=C2C#CC3=CC=CC=C3C1)CCC(NCC(NCC(N[C@@H](CC4=CC=CC=C4)C(NCC(NCOCC(N[C@@H]5C6=C7C(C(N8C7)=CC([C@](O)(C(OC9)=O)CC)=C9C8=O)=NC%10=CC(F)=C(C)C(CC5)=C%106)=O)=O)=O)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (88.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.8864 mL | 4.4320 mL | 8.8640 mL | 22.1600 mL |
| 5 mM | 0.1773 mL | 0.8864 mL | 1.7728 mL | 4.4320 mL | |
| 10 mM | 0.0886 mL | 0.4432 mL | 0.8864 mL | 2.2160 mL | |
| 15 mM | 0.0591 mL | 0.2955 mL | 0.5909 mL | 1.4773 mL | |
| 20 mM | 0.0443 mL | 0.2216 mL | 0.4432 mL | 1.1080 mL | |
| 25 mM | 0.0355 mL | 0.1773 mL | 0.3546 mL | 0.8864 mL | |
| 30 mM | 0.0295 mL | 0.1477 mL | 0.2955 mL | 0.7387 mL | |
| 40 mM | 0.0222 mL | 0.1108 mL | 0.2216 mL | 0.5540 mL | |
| 50 mM | 0.0177 mL | 0.0886 mL | 0.1773 mL | 0.4432 mL | |
| 60 mM | 0.0148 mL | 0.0739 mL | 0.1477 mL | 0.3693 mL | |
| 80 mM | 0.0111 mL | 0.0554 mL | 0.1108 mL | 0.2770 mL |
DIBAC-GGFG-NH2CH2-Dxd Related Classifications
HY-147363 Related Classifications
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