Dicranin
Dicranin is a 15-lipoxygenase (15-LOX) inhibitor and bacterial agent. Dicranin inhibits the dioxygenation reaction of polyenoic fatty acids by irreversibly binding to lipoxygenase. Dicranin can be isolated from the phospholipids of Philonotis fontana, Leptodontium paradoxum, and Dicranum scoparium. Dicranin can be used in studies related to Gram-positive bacterial infections.
For research use only. We do not sell to patients.
- CAS No.: 61481-30-9
- Formula: C18H26O2
- Molecular Weight:274.40
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
15-LOX |
In Vitro
Dicranin (0.36-3.6 μM) potently inhibits soybean 15-lipoxygenase, achieving 100% inhibition at 3.6 μM and 37% inhibition at 0.36 μM[2].
Dicranin (10-1000 μg/disc) inhibits growth of Gram-positive bacteria (Bacillus stearothermophilus, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis) in disc diffusion assays, with the strongest activity against Streptococcus faecalis (inhibition down to 10 μg/disc), and no activity against tested Gram-negative bacteria at 1000 μg/disc[2].
Dicranin (10-200 μg/mL; up to 24 h) acts as a bactericide against Bacillus cereus (down to 150 μg/mL) and Bacillus stearothermophilus (at 200 μg/mL), with time-dependent bactericidal/bacteriostatic effects at intermediate concentrations, weak growth inhibition against Bacillus stearothermophilus at 10 μg/mL, no activity against Bacillus cereus at 10 μg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 61481-30-9
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Molecular Weight 274.40
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Formula C18H26O2
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SMILES
CC/C=C\C/C=C\C/C=C\CC#CCCCCC(O)=O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dicranin Related Classifications
HY-187674 Related Classifications
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