Diosgenin glucoside
Based on 1 Customer Validation
Diosgenin glucoside, a saponin compound extracted from Trillium tschonoskii, provides neuroprotection by regulating microglial M1 polarization. Diosgenin glucoside protects against spinal cord injury by regulating autophagy and alleviating apoptosis .
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 14144-06-0
- Formula: C33H52O8
- Molecular Weight:576.76
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Natural Product Library
HY-L021
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Autophagy Compound Library
HY-L029
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Glycoside Compound Library
HY-L042
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Lipid Compound Library
HY-L043
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Orally Active Compound Library
HY-L061
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Traditional Chinese Medicine Active Compound Library
HY-L065
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Neuroprotective Compound Library
HY-L070
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Anti-Cancer Metabolism Compound Library
HY-L083
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Food-Sourced Compound Library
HY-L094
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Anti-inflammatory Traditional Chinese Medicine Active Compound Library
HY-L114
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Plant-Sourced Natural Product Library
HY-L115
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Human Metabolite Library
HY-L123
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Natural Product Library Plus
HY-L021P
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Natural Product and Natural Product-Like Compound Library
HY-L021M
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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High-Throughput Natural Product Library
HY-L206
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Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library
HY-L209
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Mass Spectrometry Human Metabolite Library
HY-L215
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Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library
HY-L223
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Diarrhea-Related Traditional Chinese Medicine Active Compound Library
HY-L224
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Mongolian Medicine Compound Library
HY-L238
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Natural Product Diversity Scaffold Library
HY-L245
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Tonifying Traditional Chinese Medicine Monomer Compound Library
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Mass Spectrometry Natural Product Library
HY-L262
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
30.3 μM
Compound: Diosgenin-glucopyranoside
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Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 24974349] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Spinal cord injury (SCI) rats models[2]
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Dosage:100 mg/kg
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Administration:po, once daily for 21 days
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Result:Reduced spinal cord tissue damage, increased the number of motor neurons, and reduced the mitochondrial swelling.
Reduced the number of apoptotic cells.
Increased the LC3-II/LC3-I ratio and Beclin1 expression, and decreased p62 expression.
Chemical Information
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CAS No. 14144-06-0
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Appearance Solid
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Molecular Weight 576.76
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Formula C33H52O8
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Color White to off-white
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SMILES
C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C5[C@@]6(CC[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]7O)O)([H])O[C@@H]7CO)C5)C)([H])[C@]6([H])CC2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (173.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Int Immunopharmacol. 2017 Sep;50:22-29. doi: 10.1016/j.intimp.2017.06.008. Epub 2017 Jun 14. [Content Brief]
[2]. Chen XB, et al. Diosgenin Glucoside Protects against Spinal Cord Injury by Regulating Autophagy and Alleviating Apoptosis. Int J Mol Sci. 2018 Aug 2;19(8). pii: E2274. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7338 mL | 8.6691 mL | 17.3382 mL | 43.3456 mL |
| 5 mM | 0.3468 mL | 1.7338 mL | 3.4676 mL | 8.6691 mL | |
| 10 mM | 0.1734 mL | 0.8669 mL | 1.7338 mL | 4.3346 mL | |
| 15 mM | 0.1156 mL | 0.5779 mL | 1.1559 mL | 2.8897 mL | |
| 20 mM | 0.0867 mL | 0.4335 mL | 0.8669 mL | 2.1673 mL | |
| 25 mM | 0.0694 mL | 0.3468 mL | 0.6935 mL | 1.7338 mL | |
| 30 mM | 0.0578 mL | 0.2890 mL | 0.5779 mL | 1.4449 mL | |
| 40 mM | 0.0433 mL | 0.2167 mL | 0.4335 mL | 1.0836 mL | |
| 50 mM | 0.0347 mL | 0.1734 mL | 0.3468 mL | 0.8669 mL | |
| 60 mM | 0.0289 mL | 0.1445 mL | 0.2890 mL | 0.7224 mL | |
| 80 mM | 0.0217 mL | 0.1084 mL | 0.2167 mL | 0.5418 mL | |
| 100 mM | 0.0173 mL | 0.0867 mL | 0.1734 mL | 0.4335 mL |
Diosgenin glucoside Related Classifications
HY-N0730 Related Classifications
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