Diquat dibromide hydrate

Name: Diquat dibromide hydrate
Brand: MedChemExpress
SKU: HY-136372
Rating: 5.0 (3 reviews)

Cat. No.: HY-136372 Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
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Diquat dibromide hydrate is a comprehensive herbicide. Diquat dibromide hydrate increases the production of ROS and triggers mitophagy. Diquat dibromide hydrate generates free radicals such as superoxide anions through redox cycles, which induce oxidative stress. Diquat dibromide hydrate is cytotoxic, reproductive, and neurotoxic. Diquat dibromide hydrate is used in cotton, soybean, and other crops to combat noxious weeds.

For research use only. We do not sell to patients.

Diquat dibromide hydrate Chemical Structure

CAS No. : 6385-62-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Diquat dibromide hydrate:

Diquat dibromide In-stock
Diquat dibromide hydrate (Standard) Get quote

3 Publications Citing Use of MCE Diquat dibromide hydrate

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Diquat (10-20 μM; 20 h) dibromide hydrate causes cell death in SH-SY5Y cells^[3].
Diquat (50 μM) dibromide hydrate induces axonal degeneration in cultured dorsal root ganglion (DRG) neurons^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Diquat (10 mg/kg bodyweight; i.p.) dibromide hydrate induces oxidative stress, increases intestinal permeability, impairs mitochondrial function, and triggers mitophagy in piglets^[2].
Diquat dibromide hydrate (25 mg/L in drinking water; 10-week treatment) reduces beak red chroma and total plasma carotenoids, and increases lipophilic antioxidant capacity in adult male zebra finches^[4].
Diquat (8-12 mg/kg; i.p.; twice a week; 4 weeks) dibromide hydrate causes reproductive toxicity in female mice^[6].
Diquat (10 mg/kg; i.p.; twice a week; for 6 weeks) dibromide hydrate shows neurotoxic effects in male C57/BL-6 mice, inducing motor dysfunctions (such as bradykinesia, gait abnormalities, decreased swimming ability, etc.)^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Twelve male Duroc × Landrace × Yorkshire 35-d-old pigs (weaned at 21 d of age), with an average body weight of 9.6 kg, used as a model for studying the effect of Diquat-induced oxidative stress on intestinal barrier, mitochondrial function, and mitophagy^[2].
Dosage:	10 mg/kg BW (dissolved in 0.9% NaCl solution)
Administration:	Intraperitoneal injection
Result:	Significantly decreased ADFI and ADG. Impaired the intestinal barrier function, as indicated by a decrease in TER, an increase in FD4 flux, and a reduction in the protein abundance of claudin-1, occludin, and zonula occludens-1. Caused mitochondrial dysfunction, with increased ROS production, decreased membrane potential in intestinal mitochondria, and a decrease in the mRNA abundance of genes related to mitochondrial biogenesis and function. Triggered mitophagy, as shown by the increased expression of mitophagy-related proteins and the increased ratio of LC3-II to LC3-I.

Animal Model:	Female ICR mice^[6]
Dosage:	8 mg/kg or 12 mg/kg (dissolved in normal saline)
Administration:	Intraperitoneal injection, twice a week, for 4 weeks
Result:	Significantly reduced ovary weights compared with control mice. Decreased the percentage of normal GV oocytes and polar body extrusion. Significantly increased ROS production in follicular granulosa cells and oocytes. Decreased the activities of antioxidant enzymes (T-SOD, GPx and CAT) in the serum, and increased the MDA content. Reduced the average numbers of fetus and litter size on GD 7.5 d.

Molecular Weight

362.06

Formula

C₁₂H₁₄Br₂N₂O

CAS No.

6385-62-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

C12=CC=CC=[N+]1CC[N+]3=CC=CC=C23.[Br-].[Br-].O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (345.25 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7620 mL	13.8099 mL	27.6197 mL
5 mM	0.5524 mL	2.7620 mL	5.5239 mL
10 mM	0.2762 mL	1.3810 mL	2.7620 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

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Data Sheet (284 KB) SDS (479 KB)

COA (255 KB) HNMR (249 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Moreland D E. Mechanisms of action of herbicides. 1980. [Content Brief]

[2]. Nisar R, et al. Diquat causes caspase-independent cell death in SH-SY5Y cells by production of ROS independently of mitochondria. Arch Toxicol. 2015 Oct;89(10):1811-25. [Content Brief]

[3]. Zhang JQ, et al. Chronic Exposure to Diquat Causes Reproductive Toxicity in Female Mice. PLoS One. 2016 Jan 19;11(1):e0147075. [Content Brief]

[4]. Tomášek O, et al. Opposing effects of oxidative challenge and carotenoids on antioxidant status and condition-dependent sexual signalling. Sci Rep. 2016 Mar 22;6:23546. [Content Brief]

[5]. Singh P, et al. Sirtuin-2 Protects Neural Cells from Oxidative Stress and Is Elevated in Neurodegeneration. Parkinsons Dis. 2017;2017:2643587. [Content Brief]

[6]. Cao S, et al. Diquat-induced oxidative stress increases intestinal permeability, impairs mitochondrial function, and triggers mitophagy in piglets. J Anim Sci. 2018 May 4;96(5):1795-1805. [Content Brief]

[7]. Acar A. In vivo toxicological assessment of Diquat dibromide hydrate: cytotoxic, genotoxic, and biochemical approach. Environ Sci Pollut Res Int. 2021;28(34):47550-47561. [Content Brief]

[8]. Moreland D E. Mechanisms of action of herbicides. 1980.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.7620 mL	13.8099 mL	27.6197 mL	69.0493 mL
	5 mM	0.5524 mL	2.7620 mL	5.5239 mL	13.8099 mL
	10 mM	0.2762 mL	1.3810 mL	2.7620 mL	6.9049 mL
	15 mM	0.1841 mL	0.9207 mL	1.8413 mL	4.6033 mL
	20 mM	0.1381 mL	0.6905 mL	1.3810 mL	3.4525 mL
	25 mM	0.1105 mL	0.5524 mL	1.1048 mL	2.7620 mL
	30 mM	0.0921 mL	0.4603 mL	0.9207 mL	2.3016 mL
	40 mM	0.0690 mL	0.3452 mL	0.6905 mL	1.7262 mL
	50 mM	0.0552 mL	0.2762 mL	0.5524 mL	1.3810 mL
	60 mM	0.0460 mL	0.2302 mL	0.4603 mL	1.1508 mL
	80 mM	0.0345 mL	0.1726 mL	0.3452 mL	0.8631 mL
	100 mM	0.0276 mL	0.1381 mL	0.2762 mL	0.6905 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.