Castanospermine
Based on 6 publication(s) in Google Scholar
Castanospermine is a natural alkaloid that can be extracted from black beans or the Moreton Bay chestnut tree (Castanospermum australae). Castanospermine is an α/ β-glucosidase inhibitor. Castanospermine has anti-inflammatory, antiviral replication and anti-metastatic effects on prostate cancer. Castanospermine can be used as an immunosuppressant to prevent transplant rejection.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 79831-76-8
- Formula: C8H15NO4
- Molecular Weight:189.21
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Castanospermine
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Biological Activity
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Cell Line
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Type | Value | Description | References |
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| JM1 | IC50 |
7.3 μM
Compound: Castanospermine
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Tested for antiviral activity of compound against HIV (Strain GB 8) in JM cells
Tested for antiviral activity of compound against HIV (Strain GB 8) in JM cells
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10.1016/S0960-894X(01)80958-6 |
| JM1 | IC50 |
8.8 μM
Compound: Castanospermine
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Tested for antiviral activity of compound against HIV (Strain GB 8) in JM cells
Tested for antiviral activity of compound against HIV (Strain GB 8) in JM cells
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10.1016/S0960-894X(01)80958-6 |
Castanospermine (0.01-1000 μM, 48 h) inhibits the production of DEN infectious virus in a dose-dependent manner and slows the electrophoretic mobility of DEN prM (a glycosylated structural protein) in BGK-21 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BHK-21
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Concentration:100, 500 μM
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Incubation Time:48 h
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Result:Reduced marker gene expression or propagation of WNV or DEN replicons modestly by up to 20 to 40%, respectively. Had small effects on the secretion of infectious WNV or viral particles containing WNV RNA but decreased the amount of DEN viral RNA and infectious virus.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:A/J mice model[1]
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Dosage:10, 50, 250 mg/kg
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Administration:i.p.
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Result:Decreased secretion and virus infectivity, and increased survival rate of mice infected with DEN virus.
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Animal Model:Acute pancreatitis (AP) rat model[2]
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Dosage:10, 50, 100, 200, 500 mg/kg
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Administration:i.p.
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Result:The extent and severity of the pancreatic injury were significantly decreased. Decreased the interleukin production in serum and NF-kB activation. Increased the level of TNF-a, ICAM-1 and VCAM-1.
Chemical Information
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CAS No. 79831-76-8
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Appearance Solid
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Molecular Weight 189.21
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Formula C8H15NO4
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Color White to off-white
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SMILES
O[C@@H]1[C@@H](O)[C@H](O)[C@]([C@@H](O)CC2)([H])N2C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Nat Commun
Self-cleavage of the GAIN domain of adhesion G protein-coupled receptors requires multiple domain-extrinsic factors. [Abstract]2025 Oct 1;16(1):8736. PMID: 41034233 -
Nat Commun
FRET imaging of glycoRNA on small extracellular vesicles enabling sensitive cancer diagnostics. [Abstract]2025 Apr 10;16(1):3391. PMID: 40210865 -
Cell Chem Biol
2026 Jun 16:S2451-9456(26)00196-0. PMID: 42302779 -
J Virol
Disruption of spike protein N-glycosylation induces its endoplasmic reticulum retention and attenuates SARS-CoV-2 infectivity. [Abstract]2026 Apr 21;100(4):e0027026. PMID: 41910405 -
Glycobiology
2021 May 3;31(4):378-384. PMID: 32985653 -
Solvent & Solubility
H2O : 100 mg/mL (528.51 mM; Need ultrasonic)
DMSO : 100 mg/mL (528.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 120 mg/mL (634.22 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Whitby K, et al. Castanospermine, a potent inhibitor of dengue virus infection in vitro and in vivo. J Virol. 2005 Jul;79(14):8698-706. [Content Brief]
[2]. Hong YP, et al. Effects of Castanospermine on Inflammatory Response in a Rat Model of Experimental Severe Acute Pancreatitis. Arch Med Res. 2016 Aug;47(6):436-445. [Content Brief]
[3]. Elbein AD. Glycosidase inhibitors: inhibitors of N-linked oligosaccharide processing. FASEB J. 1991 Dec;5(15):3055-63 [Content Brief]
[4]. den Dulk M, et al. Combined donor leucocyte administration and immunosuppressive drug treatment for survival of rat heart allografts. Transpl Immunol. 2004 Nov;13(3):177-84. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.2851 mL | 26.4257 mL | 52.8513 mL | 132.1283 mL |
| 5 mM | 1.0570 mL | 5.2851 mL | 10.5703 mL | 26.4257 mL | |
| 10 mM | 0.5285 mL | 2.6426 mL | 5.2851 mL | 13.2128 mL | |
| 15 mM | 0.3523 mL | 1.7617 mL | 3.5234 mL | 8.8086 mL | |
| 20 mM | 0.2643 mL | 1.3213 mL | 2.6426 mL | 6.6064 mL | |
| 25 mM | 0.2114 mL | 1.0570 mL | 2.1141 mL | 5.2851 mL | |
| 30 mM | 0.1762 mL | 0.8809 mL | 1.7617 mL | 4.4043 mL | |
| 40 mM | 0.1321 mL | 0.6606 mL | 1.3213 mL | 3.3032 mL | |
| 50 mM | 0.1057 mL | 0.5285 mL | 1.0570 mL | 2.6426 mL | |
| 60 mM | 0.0881 mL | 0.4404 mL | 0.8809 mL | 2.2021 mL | |
| 80 mM | 0.0661 mL | 0.3303 mL | 0.6606 mL | 1.6516 mL | |
| 100 mM | 0.0529 mL | 0.2643 mL | 0.5285 mL | 1.3213 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Castanospermine Related Classifications
HY-N2022 Related Classifications
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