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Cat. No.: HY-N14862

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Midecamycin A2

Midecamycin A2 is a macrolide compound with strong anti-Gram-positive bacteria and weak anti-Gram-negative bacteria, mycobacteria and fungi activity.

For research use only. We do not sell to patients.

CAS No.: 35457-81-9
Formula: C₄₂H₆₉NO₁₅
Molecular Weight:827.99

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

Midecamycin A2 Chemical Structure

CAS No.: 35457-81-9

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Midecamycin A2 is a macrolide compound with strong anti-Gram-positive bacteria and weak anti-Gram-negative bacteria, mycobacteria and fungi activity^[1].

Chemical Information

CAS No. 35457-81-9
Molecular Weight 827.99
Formula C₄₂H₆₉NO₁₅
SMILES

C[C@H]1OC(C[C@@H](OC(CC)=O)[C@H](OC)[C@@H](O[C@H]2O[C@H](C)[C@@H](O[C@H]3O[C@@H](C)[C@H](OC(CCC)=O)[C@@](O)(C)C3)[C@H](N(C)C)[C@H]2O)[C@@H](CC=O)C[C@@H](C)[C@@H](O)/C=C\C=C\C1)=O
Structure Classification
- Antibiotics
- Macrolide Antibiotics
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Histomorphology

Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. X Zhu, et al. Subcloning and expression of midecamycin polyketide synthase genes from Streptomyces mycarofaciens 1748. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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