ErbB-1/ErbB-2 tyrosine kinase-IN-1
ErbB-1/ErbB-2 tyrosine kinase-IN-1 is a dual ErbB-1/ErbB-2 tyrosine kinase inhibitor with IC50 values of 0.027 μM and 0.026 μM. ErbB-1/ErbB-2 tyrosine kinase-IN-1 targets the ATP binding region of ErbB-1 and ErbB-2 tyrosine kinases. ErbB-1/ErbB-2 tyrosine kinase-IN-1 inhibits cancer cells proliferation.
For research use only. We do not sell to patients.
- CAS No.: 231277-83-1
- Formula: C29H28N4O4S
- Molecular Weight:528.63
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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- Purity & Documentation
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Biological Activity
Description
In Vitro
ErbB-1/ErbB-2 tyrosine kinase-IN-1 (Compound 2) potently inhibits ErbB-1 and ErbB-2 tyrosine kinases with IC50 values of 0.027 μM and 0.026 μM, respectively[1].
ErbB-1/ErbB-2 tyrosine kinase-IN-1 (72 h) inhibits proliferation of HN5, BT474, and N87 tumor cells with IC50 values of 0.65 μM, 0.28 μM, and 0.87 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 231277-83-1
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Molecular Weight 528.63
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Formula C29H28N4O4S
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SMILES
O=S(=O)(C)CCNCC=1C=COC1C=2C=CC3=NC=NC(NC4=CC=C(OCC=5C=CC=CC5)C=C4)=C3C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ErbB-1/ErbB-2 tyrosine kinase-IN-1 Related Classifications
HY-123726 Related Classifications
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