Exoenzyme C3, clostridium botulinum
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Exoenzyme C3, clostridium botulinum, is a mono-ADP-ribosylating enzyme. Exoenzyme C3, clostridium botulinum specifically modifies RhoA, B, and C by transferring ADP-ribose to them, thereby inactivating these GTPases. Exoenzyme C3, clostridium botulinum can induce neuronal axonal and dendritic growth, inhibit macrophage migration, and regulate cytoskeletal dynamics. Exoenzyme C3, clostridium botulinum can be used in the research of spinal cord injury and diabetic painful neuropathy.
For research use only. We do not sell to patients.
- CAS No.: 58319-92-9
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Exoenzyme C3, Clostridium botulinum (10 nM-1 μM; 7-24 h) induces bipolar protrusions in J774A.1 macrophages via RhoA ADP-ribosylation[2].
Exoenzyme C3, Clostridium botulinum (1 μM; 72 h) alters the abundance of 55 proteins in SH-SY5Y cells[3].
Exoenzyme C3, Clostridium botulinum (1 μM; 24-144 h) causes significant proteome changes in HT22 cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Normal and Streptozotocin (HY-13753)-induced diabetic male ICR mice (weighing about 20 g) aged 4 weeks[5]
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Dosage:1 pg, 3 pg, 10 pg
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Administration:Intrathecal injection
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Result:Dose-dependently prolonged the tail-flick latency of diabetic mice at 24, 48 and 72 h after treatment and increased their mechanical threshold at 48 h after treatment.
Had no effect on the tail-flick latency and mechanical threshold of non-diabetic mice.
Chemical Information
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CAS No. 58319-92-9
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Appearance Solid
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Color White to off-white
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SMILES
[Exoenzyme C3, clostridium botulinum]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Ingo Just, et al. Therapeutic Effects of Clostridium Botulinum C3 Exoenzyme. Naunyn Schmiedebergs Arch Pharmacol. 2011 Mar;383(3):247-52. [Content Brief]
[2]. Rotsch J, et al. Inhibition of macrophage migration by C. botulinum exoenzyme C3. Naunyn Schmiedebergs Arch Pharmacol. 2012 Sep;385(9):883-90. [Content Brief]
[3]. Muetzelburg MV, et al. Identification of biomarkers indicating cellular changes after treatment of neuronal cells with the C3 exoenzyme from Clostridium botulinum using the iTRAQ protocol and LC-MS/MS analysis. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 1;877(13):1344-51. [Content Brief]
[4]. Schröder A, et al. Proteome Alterations of Hippocampal Cells Caused by Clostridium botulinum C3 Exoenzyme. J Proteome Res. 2015 Nov 6;14(11):4721-33. [Content Brief]
[5]. Ohsawa M, et al. RhoA/Rho kinase pathway contributes to the pathogenesis of thermal hyperalgesia in diabetic mice. Pain. 2011 Jan;152(1):114-122. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Exoenzyme C3, clostridium botulinum Related Classifications
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