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Cat. No.: HY-N11430

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F1839-I

F1839-I is a compound that can be isolated from Stachybotrys. F1839-I has weak cytotoxicity and anti-HIV activity with an IC₅₀ value of 15.6 μM.

For research use only. We do not sell to patients.

CAS No.: 159096-49-8
Formula: C₂₃H₃₂O₄
Molecular Weight:372.50

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

F1839-I Chemical Structure

CAS No.: 159096-49-8

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Protocols (3)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

F1839-I is a compound that can be isolated from Stachybotrys. F1839-I has weak cytotoxicity and anti-HIV activity with an IC₅₀ value of 15.6 μM^[1].

All HIV Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BGC-823	IC₅₀	21.9 μM Compound: 1; F1839-I	Cytotoxicity against human BGC823 cells assessed as reduction in cell viability after 96 hrs by MTT assay Cytotoxicity against human BGC823 cells assessed as reduction in cell viability after 96 hrs by MTT assay	[PMID: 28530828]
HepG2	IC₅₀	18.4 μM Compound: 1; F1839-I	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 96 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 96 hrs by MTT assay	[PMID: 28530828]
NCI-H460	IC₅₀	15.8 μM Compound: 1; F1839-I	Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 96 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 96 hrs by MTT assay	[PMID: 28530828]

In Vitro

F1839-I is weakly active against the HepG2, NCI-H460 and BGC823 cells with IC₅₀ values of 18.4, 15.8 and 21.9 μM, respectively.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 159096-49-8
Molecular Weight 372.50
Formula C₂₃H₃₂O₄
SMILES

C[C@@]12[C@@]3([C@@H](CC[C@@]1([H])C(C)([C@@H](CC2)O)C)C)OC4=C(C=O)C(C)=CC(O)=C4C3
Structure Classification
- Phenols
- Monophenols
Initial Source
- Microorganisms
Stachybotrys chartarum
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhao J, et al. Stachybotrysins A-G, Phenylspirodrimane Derivatives from the Fungus Stachybotrys chartarum. J Nat Prod. 2017 Jun 23;80(6):1819-1826. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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