FBBBE
Based on 1 Customer Validation
FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm).
For research use only. We do not sell to patients.
- Purity: 99.9%
- CAS No.: 1522117-83-4
- Formula: C46H46B2O9
- Molecular Weight:764.47
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
H2O2[1]
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. The RAW 264.7 cells are seeded at 250,000 in glass bottom 35 mm dishes and post-adhering (5 h).
2. Some dishes are treated with 1 μg/mL of LPS for 24 h. Other dishes are treated the next day after plating with either 100 μM H2O2 for 1 h or PBS for 1 h.
3. Cells are washed twice with PBS and fixed in ice-cold 95% ethanol for 15 min.
3. Cells are twice rinsed with PBS and incubated with either 50 μM probe (FBBBE) for 1 h at room temperature.
4. Cells are washed twice with PBS.
5. Observed under a confocal laser scanning microscope.
6. Images were processed using ImageJ software.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
512
480
Chemical Information
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CAS No. 1522117-83-4
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Appearance Solid
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Molecular Weight 764.47
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Formula C46H46B2O9
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Color Off-white to light yellow
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SMILES
O=C1OC2(C3=C1C=CC=C3)C4=CC=C(C=C4OC5=CC(OCC6=CC=C(B7OC(C)(C(C)(C)O7)C)C=C6)=CC=C52)OCC8=CC=C(B9OC(C)(C(C)(C)O9)C)C=C8
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMF : 30 mg/mL (39.24 mM; Need ultrasonic and warming)
DMSO : 25 mg/mL (32.70 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / DMF | 1 mM | 1.3081 mL | 6.5405 mL | 13.0810 mL | 32.7024 mL |
| 5 mM | 0.2616 mL | 1.3081 mL | 2.6162 mL | 6.5405 mL | |
| 10 mM | 0.1308 mL | 0.6540 mL | 1.3081 mL | 3.2702 mL | |
| 15 mM | 0.0872 mL | 0.4360 mL | 0.8721 mL | 2.1802 mL | |
| 20 mM | 0.0654 mL | 0.3270 mL | 0.6540 mL | 1.6351 mL | |
| 25 mM | 0.0523 mL | 0.2616 mL | 0.5232 mL | 1.3081 mL | |
| 30 mM | 0.0436 mL | 0.2180 mL | 0.4360 mL | 1.0901 mL |
FBBBE Related Classifications
HY-D1693 Related Classifications
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