MMP Substrate

MMP-1 Substrate is a matrix metalloproteinase-1 (MMP-1) selective substrate that can be used for the fluorometric determination of MMP-1 enzymatic activity.

TNO211 (Dabcyl-GABA-Pro-Gln-Gly-Leu-Glu (EDANS)-Ala-Lys-NH2) TFA is a fluorescent peptide substrate used to detect matrix metalloproteinase (MMP) activity. TNO211 TFA contains a Gly-Leu hydrolysis site recognized by MMPs and is linked to the fluorescent group EDANS and the quenching group Dabcyl, respectively. In the intact substrate, Dabcyl quenches the fluorescence of EDANS; when active MMPs hydrolyze the Gly-Leu peptide bond, the two groups separate, the fluorescence quenching effect weakens, and EDANS restores its fluorescence signal. The increase in fluorescence intensity can be used to reflect the degree of substrate hydrolysis and MMP activity. TNO211 TFA has good selectivity for MMPs and is not readily hydrolyzed by non-MMP proteases such as plasmin, urokinase-type plasminogen activator (u-PA), and elastase (Ex/Em = 340/485 nm).

Mca-AQTLTL-Dpa-RSSSQ-NH2 is a fluorescent substrate peptide for TNF-α converting enzyme (TACE), with its peptide sequence spanning the TACE cleavage site of tmTNF. In its intact state, the fluorescence of Mca-AQTLTL-Dpa-RSSSQ-NH2 is quenched; upon cleavage by TACE, a fluorescent signal is released, thereby reflecting TACE enzymatic activity. Mca-AQTLTL-Dpa-RSSSQ-NH2 can be used for studies related to TACE activity and TACE inhibition.

TNO211 (Dabcyl-GABA-Pro-Gln-Gly-Leu-Glu (EDANS)-Ala-Lys-NH2) is a fluorescent peptide substrate used to detect matrix metalloproteinase (MMP) activity. TNO211 contains a Gly-Leu hydrolysis site recognized by MMPs and is linked to the fluorescent group EDANS and the quenching group Dabcyl, respectively. In the intact substrate, Dabcyl quenches the fluorescence of EDANS; when active MMPs hydrolyze the Gly-Leu peptide bond, the two groups separate, the fluorescence quenching effect weakens, and EDANS restores its fluorescence signal. The increase in fluorescence intensity can be used to reflect the degree of substrate hydrolysis and MMP activity. TNO211 has good selectivity for MMPs and is not readily hydrolyzed by non-MMP proteases such as plasmin, urokinase-type plasminogen activator (u-PA), and elastase (Ex/Em = 340/485 nm).

BHQ‑3 carboxylic acid is an azo‑containing dark fluorescence quencher. BHQ‑3 carboxylic acid is conjugated to an MMP‑cleavable peptide and paired with a fluorophore to form an activatable probe for MMP activity detection in vitro. BHQ‑3 carboxylic acid shows maximal absorption in the 620 to 730 nm range. BHQ‑3 carboxylic acid–based fluorescent probes can be used in the research of arthritis and tumor‑related pathological processes..

TG-MMP-Lys is an MMP-sensitive crosslinking peptide. TG-MMP-Lys is promising for research of fibrotic diseases (e.g., liver fibrosis).

TNO003 is a selective fluorogenic substrate for stromelysin-1 (MMP-3). TNO003 has higher selectivity for MMP-3 than MMP-1, MMP-2, MMP-7, MMP-8, MMP-9 and MMP-13. TNO003 can be used for the determination of stromelysin activity (Ex/Em = 360/490 nm).

TNO003 TFA is a selective fluorogenic substrate for stromelysin-1 (MMP-3). TNO003 TFA has higher selectivity for MMP-3 than MMP-1, MMP-2, MMP-7, MMP-8, MMP-9 and MMP-13. TNO003 TFA can be used for the determination of stromelysin activity (Ex/Em = 360/490 nm).