U18666A
Based on 29 publication(s) in Google Scholar
U18666A, an intra-cellular cholesterol transport inhibitor, inhibits replication of Ebola virus, dengue virus, and human hepatitis C virus.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 3039-71-2
- Formula: C25H42ClNO2
- Molecular Weight:424.06
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) U18666A
More- Cell. 2026 Feb 5;189(3):818-831.e19. [Abstract]
- Nat Microbiol. 2026 Jun;11(6):1677-1695. [Abstract]
- Nat Commun. 2026 Feb 12;17(1):1214. [Abstract]
- Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]
- Mol Cell. 2025 Sep 18;85(18):3486-3504.e7. [Abstract]
- J Control Release. 2026 Jun 18:396:115116. [Abstract]
- Oncogene. 2026 Apr;45(15):1386-1397. [Abstract]
- Cell Chem Biol. 2024 Mar 8:S2451-9456(24)00081-3. [Abstract]
- Transl Psychiatry. 2026 Jun 23;16(1):318. [Abstract]
- Br J Pharmacol. 2026 Apr 26. [Abstract]
- Emerg Microbes Infect. 2023 Dec;12(2):2261556. [Abstract]
- Cell Mol Life Sci. 2024 Jul 6;81(1):289. [Abstract]
- Neurobiol Dis. 2024 Nov 7:202:106728. [Abstract]
- Int J Mol Sci. 2022 Oct 1;23(19):11660. [Abstract]
- J Dairy Sci. 2023 Aug;106(8):5835-5852. [Abstract]
- Antiviral Res. 2023 Jan:209:105497. [Abstract]
- J Virol. 2026 Jun 9:e0030126. [Abstract]
- BMC Cancer. 2025 May 21;25(1):906. [Abstract]
- J Virol. 2023 Mar 30;97(3):e0004123. [Abstract]
- J Virol. 2020 Nov 23;94(24):e01350-20. [Abstract]
- J Virol. 2020 Mar 17;94(7):e01719-19. [Abstract]
- Vet Res. 2025 Jan 31;56(1):23. [Abstract]
- PLoS Negl Trop Dis. 2019 Aug 20;13(8):e0007681. [Abstract]
- Vet Microbiol. 2025 Apr 8:305:110507. [Abstract]
- Vet Microbiol. 2023 Mar:278:109668. [Abstract]
- bioRxiv. 2026 Jun 18.
- bioRxiv. 2025 Nov 21:2025.11.20.689520. [Abstract]
- bioRxiv. 2024 October 17.
- Research Square Preprint. 2023 Jun 22.
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
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ELISA
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WB
Biological Activity
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Cell Line
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Type | Value | Description | References |
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| HL-60 | IC50 |
>50 μM
Compound: U18666A
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Cytotoxicity in human HL60 cells by MTT assay
Cytotoxicity in human HL60 cells by MTT assay
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[PMID: 28964935] |
| Huh-7 | CC50 |
34.4 μM
Compound: 67
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Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay
Cytotoxicity against human HuH7 cells assessed as cell viability after 48 hrs by CellTiter Glo assay
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[PMID: 26771861] |
U18666A, the antiviral effect is found to result from two events: retarded viral trafficking in the cholesterol-loaded late endosomes/lysosomes and suppresse de novo sterol biosynthesis in treated infected cells. It is also observed an additive antiviral effect of U18666A with C75, a fatty acid synthase inhibitor, suggesting dengue virus relies on both the host cholesterol and fatty acid biosynthesis for successful replication[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3039-71-2
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Appearance Solid
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Molecular Weight 424.06
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Formula C25H42ClNO2
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Color White to off-white
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SMILES
C[C@@]12[C@]3([H])[C@](CC=C1C[C@@H](OCCN(CC)CC)CC2)([H])[C@@]4([H])[C@@](C)(C(CC4)=O)CC3.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (29)
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Journal Impact Factor
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Most Recent
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Cell
Molecular characterization of Ebola virus glycoprotein V75A substitution in the 2018-2020 epidemic. [Abstract]2026 Feb 5;189(3):818-831.e19. PMID: 41576953 -
Nat Microbiol
Lipid accumulation in tuberculosis granulomas inhibits macrophage-CD4+ T cell interactions and infection control. [Abstract]2026 Jun;11(6):1677-1695. PMID: 41933199 -
Nat Commun
Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]2026 Feb 12;17(1):1214. PMID: 41680175 -
Nat Commun
Cholesterol-rich lysosomes induced by respiratory syncytial virus promote viral replication by blocking autophagy flux. [Abstract]2024 Jul 26;15(1):6311. PMID: 39060258
U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]
Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated HEp-2 cells.
U18666A purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 26;15(1):6311. [Abstract]
Immunocolocalization of RSV proteins (RSV-F, RSV-G, and RSV-N), cholesterol (filipin III), and LAMP-1 in mock-infected, RSV-infected (24 h post-infection), and U18666A (10 μM)-treated 16HBE cells.
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Mol Cell
mTOR inhibition reprograms cellular lipid homeostasis by inducing alternative lipid uptake and promoting cholesterol transport. [Abstract]2025 Sep 18;85(18):3486-3504.e7. PMID: 40972529 -
J Control Release
Reversing immunotherapy resistance in cold tumors by weaponizing pyroptosis with a dual-payload nanotuner. [Abstract]2026 Jun 18:396:115116. PMID: 42314991 -
Oncogene
The NPC1/USP7/p53 axis regulates cholesterol and promotes the proliferation of hepatocellular carcinoma. [Abstract]2026 Apr;45(15):1386-1397. PMID: 41882104 -
Cell Chem Biol
FLEX: genetically encodable enzymatic fluorescence signal amplification using engineered peroxidase. [Abstract]2024 Mar 8:S2451-9456(24)00081-3. PMID: 38513646 -
Transl Psychiatry
2026 Jun 23;16(1):318. PMID: 42337263 -
Br J Pharmacol
Curcumin controls lysosomal acidification and exocytosis to ameliorate lysosomal cholesterol accumulation in Niemann-Pick type C disease. [Abstract]2026 Apr 26. PMID: 42036241 -
Emerg Microbes Infect
Hepatitis B virus promotes its own replication by enhancing RAB5A-mediated dual activation of endosomal and autophagic vesicle pathways. [Abstract]2023 Dec;12(2):2261556. PMID: 37725090
U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556. [Abstract]
HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours. The levels of secreted HBsAg in the culture supernatant and intracellular HBsAg in the cell lysate were detected by ELISA.
U18666A purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(2):2261556. [Abstract]
HepG2.2.15 cells were treated with 10 μM dynasore, 2 μM U18666A, or DMSO for 48 hours, and HBcAg was detected by WB.
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Cell Mol Life Sci
Human cytomegalovirus infection impairs neural differentiation via repressing sterol regulatory element binding protein 2-mediated cholesterol biosynthesis. [Abstract]2024 Jul 6;81(1):289. PMID: 38970696
U18666A purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Jul 6;81(1):289. [Abstract]
Intracellular cholesterol contents measured in SHEDs with different treatment. Cells added U18666A (2 µg/mL), an inhibitor specific to cholesterol synthesis, were measured as positive reference.
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Neurobiol Dis
LRRK2 and RAB8A regulate cell death after lysosomal damage in macrophages through cholesterol-related pathways. [Abstract]2024 Nov 7:202:106728. PMID: 39521098 -
Int J Mol Sci
Hepatocyte-Derived Prostaglandin E2-Modulated Macrophage M1-Type Polarization via mTOR-NPC1 Axis-Regulated Cholesterol Transport from Lysosomes to the Endoplasmic Reticulum in Hepatitis B Virus x Protein-Related Nonalcoholic Steatohepatitis. [Abstract]2022 Oct 1;23(19):11660. PMID: 36232960 -
J Dairy Sci
Regulation of cholesterol metabolism during high fatty acid-induced lipid deposition in calf hepatocytes. [Abstract]2023 Aug;106(8):5835-5852. PMID: 37419743 -
Antiviral Res
Targeting 7-dehydrocholesterol reductase against EV-A71 replication by upregulating interferon response. [Abstract]2023 Jan:209:105497. PMID: 36528172
U18666A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jan:209:105497. [Abstract]
Pretreatment with AY9944 (15, 30 µM), U18666A (5, 10 µM), lovastatin and MβCD for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.
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J Virol
The cholesterol transporter Niemann-Pick C1 facilitates the entry of porcine epidemic diarrhea coronavirus. [Abstract]2026 Jun 9:e0030126. PMID: 42262129 -
BMC Cancer
APOL4-mediated intracellular cholesterol trafficking is essential for glioblastoma cell growth. [Abstract]2025 May 21;25(1):906. PMID: 40399861 -
J Virol
Bombyx mori Nucleopolyhedrovirus Hijacks Multivesicular Body as an Alternative Envelopment Platform for Budded Virus Egress. [Abstract]2023 Mar 30;97(3):e0004123. PMID: 36916914 -
J Virol
Interferon-Induced Transmembrane Protein 3 Is a Virus-Associated Protein Which Suppresses Porcine Reproductive and Respiratory Syndrome Virus Replication by Blocking Viral Membrane Fusion. [Abstract]2020 Nov 23;94(24):e01350-20. PMID: 32999030 -
J Virol
The Late Domain of Prototype Foamy Virus Gag Facilitates Autophagic Clearance of Stress Granules by Promoting Amphisome Formation. [Abstract]2020 Mar 17;94(7):e01719-19. PMID: 31969431 -
Vet Res
The cholesterol metabolite 25-hydroxycholesterol suppresses porcine deltacoronavirus via lipophagy inhibition and mTORC1 modulation. [Abstract]2025 Jan 31;56(1):23. PMID: 39891192 -
PLoS Negl Trop Dis
Identification of anti-flaviviral drugs with mosquitocidal and anti-Zika virus activity in Aedes aegypti. [Abstract]2019 Aug 20;13(8):e0007681. PMID: 31430351 -
Vet Microbiol
Cholesterol-dependent Nsp5-endosomes co-trafficking to lysosomes facilitates porcine reproductive and respiratory syndrome virus replication by activating autophagy. [Abstract]2025 Apr 8:305:110507. PMID: 40215803 -
Vet Microbiol
25-hydroxycholesterol inhibits classical swine fever virus entry into porcine alveolar macrophages by depleting plasma membrane cholesterol. [Abstract]2023 Mar:278:109668. PMID: 36709687 -
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bioRxiv
2025 Nov 21:2025.11.20.689520. PMID: 41332603 -
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Solvent & Solubility
DMSO : 20.83 mg/mL (49.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5.56 mg/mL (13.11 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 5 mg/mL (11.79 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Poh MK, et al. U18666A, an intra-cellular cholesterol transport inhibitor, inhibits dengue virus entry and replication. Antiviral Res. 2012 Jan;93(1):191-8. [Content Brief]
[2]. Cenedella RJ, et al. Cholesterol synthesis inhibitor U18666A and the role of sterol metabolism and trafficking in numerous pathophysiological processes. Lipids. 2009 Jun;44(6):477-87. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.3582 mL | 11.7908 mL | 23.5816 mL | 58.9539 mL |
| 5 mM | 0.4716 mL | 2.3582 mL | 4.7163 mL | 11.7908 mL | |
| 10 mM | 0.2358 mL | 1.1791 mL | 2.3582 mL | 5.8954 mL | |
| DMSO | 15 mM | 0.1572 mL | 0.7861 mL | 1.5721 mL | 3.9303 mL |
| 20 mM | 0.1179 mL | 0.5895 mL | 1.1791 mL | 2.9477 mL | |
| 25 mM | 0.0943 mL | 0.4716 mL | 0.9433 mL | 2.3582 mL | |
| 30 mM | 0.0786 mL | 0.3930 mL | 0.7861 mL | 1.9651 mL | |
| 40 mM | 0.0590 mL | 0.2948 mL | 0.5895 mL | 1.4738 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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