2. Induced Disease Models Products Metabolic Enzyme/Protease
  3. Immunology and Inflammatory Disease Models Glucose-6-Phosphate Isomerase (GPI)
  4. Arthritis Models
  5. G6PI 325-339 (human)

G6PI 325-339 (human) is an efficient inducer of arthritis in B10.Q mice. G6PI 325-339 (human) primes Th1 and Th17 cells cross-reacted with the murine G6PI protein. G6PI 325-339 (human) induces arthritis model operating through a T and B cell-dependent pathway but without antibody effector mechanisms.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

G6PI 325-339 (human)

G6PI 325-339 (human) Chemical Structure

CAS No. : 1174631-35-6

Size Price Stock Quantity
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of G6PI 325-339 (human):

G6PI 325-339 (human) Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

G6PI 325-339 (human) is an efficient inducer of arthritis in B10.Q mice. G6PI 325-339 (human) primes Th1 and Th17 cells cross-reacted with the murine G6PI protein. G6PI 325-339 (human) induces arthritis model operating through a T and B cell-dependent pathway but without antibody effector mechanisms[1].

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

G6PI can be used to induce arthritis models.

Induction of Arthritis[2]
Background
G6PI is capable of producing antibodies in the body that induce arthritis by inducing immunoactive cells to produce cytokines (such as TNF-α, IL-1β).
Specific Modeling Methods
Mice: DAB/1 • 6-10 week old
Administration: 50 μg in complete Freund's adjuvant • s.c. at the base of the tail • single dose
Note
(1) DBA/1 mice were bred and maintained under specific-pathogen free conditions in our animal facility.
(2) Mice were sacrificed on day 12 after immunization and single-cell suspensions from draining lymph nodes were prepared.
Modeling Indicators
Morphology: Showed a strong synovialitis and inflammation of the adjacent skeletal muscle , which was dominated by neutrophilic granulocytes as a sign of acute inflammation and exhibited activation of synovial fibroblasts to a lesser extent. The synovial tissue was enlarged due to the dense inflammatory infiltrate; however, bone destruction was onlyfocally observed.
Molecular changes: Increased the levels of IL-17, TNFα, RANKL, and IFNγ.
Correlated Product(s): Complete Freund's adjuvant (CFA) (HY-153808)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

1801.12

Formula

C82H117N19O21S3
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ile-Trp-Tyr-Ile-Asn-Cys-Phe-Gly-Cys-Glu-Thr-His-Ala-Met-Leu
Sequence Shortening

IWYINCFGCETHAML
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (27.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.5552 mL 2.7761 mL 5.5521 mL
5 mM 0.1110 mL 0.5552 mL 1.1104 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.5552 mL 2.7761 mL 5.5521 mL 13.8803 mL
5 mM 0.1110 mL 0.5552 mL 1.1104 mL 2.7761 mL
10 mM 0.0555 mL 0.2776 mL 0.5552 mL 1.3880 mL
15 mM 0.0370 mL 0.1851 mL 0.3701 mL 0.9254 mL
20 mM 0.0278 mL 0.1388 mL 0.2776 mL 0.6940 mL
25 mM 0.0222 mL 0.1110 mL 0.2221 mL 0.5552 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

G6PI 325-339 (human) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

G6PI 325-339 (human)1174631-35-6Glucose-6-Phosphate Isomerase (GPI)Glucose-6-Phosphate IsomeraseGPI/G6PIArthritisB10.Q miceTh1Th17G6PI proteinB cellInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
G6PI 325-339 (human)
Cat. No.:
HY-P10109
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.