DNA polymerase-IN-7
DNA polymerase-IN-7 is a noncompetitive inhibition of DNA Polymerase β with an IC50 of 112 nM and a Ki of 35 nM. DNA polymerase-IN-7 can inhibit DNA pol β lyase activity and the binding of the lyase domain of Pol β to DNA. DNA polymerase-IN-7 can be used for the research of cancer, such as cervical cancer.
For research use only. We do not sell to patients.
- Formula: C47H48N7O10P
- Molecular Weight:901.90
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
[1]|
Rat pol β 112 nM (IC50) |
DNA Polymerase |
Rat pol β 35 nM (Ki) |
In Vitro
DNA polymerase-IN-7 (Compound 28) (0.1-1 μM) inhibits dGTP incorporation activity of DNA polymerase β (Pol β) and inhibits the lyase activity of Pol β[1].
DNA polymerase-IN-7 (0.35-2 μM) exerts little effect on the binding of Pol β to DNA, but inhibits the binding of the lyase domain of Pol β to DNA[1].
DNA polymerase-IN-7 (0.1-1 μM) exhibits selectivity for X-family polymerases, with inhibitory rate of 50% for Pol β and 41% for Pol λ at 0.1 μM, and almost 100% at 1 μM[1].
DNA polymerase-IN-7 (0-100 μM, 24 h) shows no obvious cytotoxicity, maintaining cell viability at 95-100% in HeLa cells[1].
DNA polymerase-IN-7 (25 μM, 24 h) shows intracellular concentration reaching 4.0 μM in HeLa cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 901.90
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Formula C47H48N7O10P
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SMILES
O=C(C1=CC=C(C2=CC=C(C3=CC=C(C4=CC=C(O)C=C4)C=C3)C=C2)C=C1)N[C@H]5C[C@H](N6C=C(CCCNC(C7=CN8C(C=C(C)C=C8)=N7)=O)C(NC6=O)=O)O[C@@H]5COP([O-])(OC)=O.[NH4+]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DNA polymerase-IN-7 Related Classifications
HY-178063 Related Classifications
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