2-Bromohexadecanoic acid
Based on 20 publication(s) in Google Scholar
2-Bromohexadecanoic acid (2-Bromopalmitate; 2-BP) is a palmitoylation inhibitor targeting DHHC (Asp-His-His-Cys) protein palmitoyltransferase. 2-Bromohexadecanoic acid inhibits palmitoylation of GSDME-C during pyroptosis and inhibits BAK/BAX-Caspase 3-GSDME pathway-mediated pyroptosis.
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- Pureza: 99.95%
- No. CAS: 18263-25-7
- Fòrmula: C16H31BrO2
- Peso molecular:335.32
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) 2-Bromohexadecanoic acid
More- Science. 2026 Feb 26;391(6788):eads4405. [Abstract]
- Cell Metab. 2026 Feb 18:S1550-4131(26)00015-X. [Abstract]
- ACS Nano. 2025 May 20;19(19):18708-18728. [Abstract]
- Adv Sci (Weinh). 2026 Mar 26:e19791. [Abstract]
- Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]
- Theranostics. 2025 Jun 9;15(14):6665-6685. [Abstract]
- Cell Discov. 2026 Feb 24;12(1):13. [Abstract]
- J Biomed Sci. 2025 Feb 12;32(1):20. [Abstract]
- Research (Wash D C). 2025 Nov 25:8:1001. [Abstract]
- Cell Mol Biol Lett. 2025 Jan 29;30(1):14. [Abstract]
- Cancer Lett. 2026 Apr 1:642:218277. [Abstract]
- Cell Death Dis. 2026 Mar 19;17(1):323. [Abstract]
- Acta Neuropathol. 2025 May 14;149(1):48. [Abstract]
- Phytomedicine. 2025 Oct:146:157091. [Abstract]
- Ecotoxicol Environ Saf. 2025 Apr 3:295:118139. [Abstract]
- Cancer Biol Ther. 2025 Dec;26(1):2532217. [Abstract]
- J Virol. 2021 Aug 10;95(17):e0078121. [Abstract]
- Vet Microbiol. 2021 Oct:261:109177. [Abstract]
- Biochim Biophys Acta Proteins Proteom. 2025 Oct 13;1874(1):141101. [Abstract]
- SSRN. 2025 Sep 17.
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Actividad biológica
Palmitoylation promotes the release of GSDME-C from GSDME-N after cleavage, and 2-BP inhibits the palmitoylation process, while inhibiting the modification of GSDME-C increases the interaction between GSDME-C and GSDME-N. 2-BP inhibits TNFa+CHX-induced apoptosis but not total cell death[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 18263-25-7
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Appearance Solid
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Peso molecular 335.32
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Fòrmula C16H31BrO2
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Color White to yellow
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SMILES
CCCCCCCCCCCCCCC(Br)C(O)=O
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Synonyms
2-Bromopalmitic acid; 2-Bromopalmitate
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (20)
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Journal Impact Factor
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Most Recent
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Science
2026 Feb 26;391(6788):eads4405. PMID: 41747053 -
Cell Metab
2026 Feb 18:S1550-4131(26)00015-X. PMID: 41713424 -
ACS Nano
Mechanism of S-Palmitoylation in Polystyrene Nanoplastics-Induced Macrophage Cuproptosis Contributing to Emphysema through Alveolar Epithelial Cell Pyroptosis. [Abstract]2025 May 20;19(19):18708-18728. PMID: 40335889 -
Adv Sci (Weinh)
SMAD4 Palmitoylation Drives a Metabolic-Transcriptional Circuit to Promote Tumorigenesis and Confers Radiosensitivity in Pancreatic Cancer. [Abstract]2026 Mar 26:e19791. PMID: 41885390 -
Adv Sci (Weinh)
Iron Overload Mediates the Differential Cell Fate of Astrocytes from Neurons and Its Regulatory Mechanisms in Ischemic Stroke. [Abstract]2025 Nov 6:e07384. PMID: 41195589
2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]
HA cells were cultured with 10 µM 2‐BP during reoxygenation for 24 h. Western blot results showed a significant reduction in total TfR1 levels and total TfR1 palmitoylation levels.
2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]
Confocal microscopy images visually demonstrate the palmitoylation of TfR1 and its changes in localization on the cell membrane after treatment with OGD/Re and 2-BP (10 μM, 24 h).
2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 6:e07384. [Abstract]
Immunofluorescence results showed that OGD/Re treatment increased the co-localization of TfR1 and clathrin in HA cells, while 2-BP (10 μM, 24 h) decreased their co-localization.
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Theranostics
Oxidative stress-mediated PANoptosis and ferroptosis: Exploration of multimodal cell death triggered by an AIE-active nano-photosensitizer via photodynamic therapy. [Abstract]2025 Jun 9;15(14):6665-6685. PMID: 40585993
2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: Theranostics. 2025 Jun 9;15(14):6665-6685. [Abstract]
A2058 and MHCC 97H cells were treated with T-T NPs, and cell viability was assessed with or without light using 2-bromohexadecanoic acid (5 µM, 3 h).
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Cell Discov
ASCT2 palmitoylation regulated by JNK1-ZDHHC14 axis orchestrates glutamine metabolism and NSCLC progression. [Abstract]2026 Feb 24;12(1):13. PMID: 41730846 -
J Biomed Sci
PEX11B palmitoylation couples peroxisomal dysfunction with Schwann cells fail in diabetic neuropathy. [Abstract]2025 Feb 12;32(1):20. PMID: 39934809
2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2025 Feb 12;32(1):20. [Abstract]
Western blot detection of peroxisomes-related proteins under PA (400 μM) and PA (400 μM) with 2-BP (50 μM) conditions for 24 h in RSC96 cells. Tubulin was used as a loading control.
2-Bromohexadecanoic acid purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2025 Feb 12;32(1):20. [Abstract]
Representative immunofluorescence staining for PMP70 (green) under PA (400 μM) and PA (400 μM) with 2-BP (50 μM) conditions for 24 h in RSC96 cells.
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Research (Wash D C)
EVA1A Regulates Hepatic Lipid Homeostasis by Modulating CD36 Expression and Its Palmitoylation. [Abstract]2025 Nov 25:8:1001. PMID: 41306774 -
Cell Mol Biol Lett
The histone demethylase KDM5C enhances the sensitivity of acute myeloid leukemia cells to lenalidomide by stabilizing cereblon. [Abstract]2025 Jan 29;30(1):14. PMID: 39881283 -
Cancer Lett
Succinate dehydrogenase B palmitoylation promotes T cell exhaustion through the H3K27ac-PD1 axis in pancreatic cancer. [Abstract]2026 Apr 1:642:218277. PMID: 41577075 -
Cell Death Dis
2026 Mar 19;17(1):323. PMID: 41856969 -
Acta Neuropathol
2025 May 14;149(1):48. PMID: 40366450 -
Phytomedicine
Columbianadin targets TRIM7 to maintain P2X7 palmitoylation, inhibiting cuproptosis in synovial M2 macrophages. [Abstract]2025 Oct:146:157091. PMID: 40768810 -
Ecotoxicol Environ Saf
Arsenic exposure provoked prostatic PANoptosis by inducing mitochondrial dysfunction in mice and WPMY-1 cells. [Abstract]2025 Apr 3:295:118139. PMID: 40185034 -
Cancer Biol Ther
Lenvatinib promotes hepatocellular carcinoma pyroptosis by regulating GSDME palmitoylation. [Abstract]2025 Dec;26(1):2532217. PMID: 40653759 -
J Virol
Fatty Acid Synthase Is Involved in Classical Swine Fever Virus Replication by Interaction with NS4B. [Abstract]2021 Aug 10;95(17):e0078121. PMID: 34132567 -
Vet Microbiol
Lipid metabolism is a novel and practical source of potential targets for antiviral discovery against porcine parvovirus. [Abstract]2021 Oct:261:109177. PMID: 34391196 -
Biochim Biophys Acta Proteins Proteom
2025 Oct 13;1874(1):141101. PMID: 41093223 -
Solvente y solubilidad
DMSO : ≥ 125 mg/mL (372.78 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 5 mg/mL (14.91 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (267 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9822 mL | 14.9111 mL | 29.8223 mL | 74.5556 mL |
| 5 mM | 0.5964 mL | 2.9822 mL | 5.9645 mL | 14.9111 mL | |
| 10 mM | 0.2982 mL | 1.4911 mL | 2.9822 mL | 7.4556 mL | |
| 15 mM | 0.1988 mL | 0.9941 mL | 1.9882 mL | 4.9704 mL | |
| 20 mM | 0.1491 mL | 0.7456 mL | 1.4911 mL | 3.7278 mL | |
| 25 mM | 0.1193 mL | 0.5964 mL | 1.1929 mL | 2.9822 mL | |
| 30 mM | 0.0994 mL | 0.4970 mL | 0.9941 mL | 2.4852 mL | |
| 40 mM | 0.0746 mL | 0.3728 mL | 0.7456 mL | 1.8639 mL | |
| 50 mM | 0.0596 mL | 0.2982 mL | 0.5964 mL | 1.4911 mL | |
| 60 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2426 mL | |
| 80 mM | 0.0373 mL | 0.1864 mL | 0.3728 mL | 0.9319 mL | |
| 100 mM | 0.0298 mL | 0.1491 mL | 0.2982 mL | 0.7456 mL |
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