INH6
Based on 1 Customer Validation
INH6 is a potent Nek2/Hec1 inhibitor; inhibits the growth of HeLa cells with an IC50 of 2.4 μM.
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- Pureza: 99.53%
- No. CAS: 1001753-24-7
- Fòrmula: C19H18N2OS
- Peso molecular:322.42
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
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NEK2 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
2.4 μM
Compound: 6
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Cytotoxicity against human HeLa cells after 4 days by XTT assay
Cytotoxicity against human HeLa cells after 4 days by XTT assay
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[PMID: 19243176] |
| HeLa | IC50 |
3.6 μM
Compound: 2
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Antiproliferative activity against human HeLa cells assessed as cell viability after 96 hrs by CellTiter96 assay
Antiproliferative activity against human HeLa cells assessed as cell viability after 96 hrs by CellTiter96 assay
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[PMID: 24773549] |
| K562 | IC50 |
2.5 μM
Compound: 6
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Cytotoxicity against human K562 cells after 4 days by XTT assay
Cytotoxicity against human K562 cells after 4 days by XTT assay
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[PMID: 19243176] |
| K562 | IC50 |
3.2 μM
Compound: 2
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Antiproliferative activity against human K562 cells assessed as cell viability after 96 hrs by CellTiter96 assay
Antiproliferative activity against human K562 cells assessed as cell viability after 96 hrs by CellTiter96 assay
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[PMID: 24773549] |
| MDA-MB-231 | IC50 |
1.7 μM
Compound: 6
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Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay
Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay
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[PMID: 19243176] |
| MDA-MB-231 | IC50 |
3.2 μM
Compound: 2
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Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 96 hrs by CellTiter96 assay
Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 96 hrs by CellTiter96 assay
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[PMID: 24773549] |
| MDA-MB-468 | IC50 |
2.1 μM
Compound: 6
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Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay
Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay
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[PMID: 19243176] |
| MDA-MB-468 | IC50 |
2.2 μM
Compound: 2
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Antiproliferative activity against human MDA-MB-468 cells assessed as cell viability after 96 hrs by CellTiter96 assay
Antiproliferative activity against human MDA-MB-468 cells assessed as cell viability after 96 hrs by CellTiter96 assay
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[PMID: 24773549] |
Hec1 is an oncogene overly expressed in many human cancers. Small molecule INH (Inhibitor of Nek2/Hec1) targeting the Hec1 and its regulator, Nek2, in the mitotic pathway is identified to inactivate Hec1/Nek2 function mediated by protein degradation that subsequently leads to chromosome mis-segregation and cell death. INH6 treated cells exhibit increased mitotic population with multipolar spindle configurations. An increased rate of chromosome misalignment is detected upon treatment with INH6 of HeLa cells expressing the chromosome marker protein H2B-GFP. INH6 treated cells shows progressive morphological changes characteristic of dying cells (e.g., membrane bubbling), which is further confirmed by cell cycle profiling with FACS analysis. Approximately 20% of INH6 treated cells are apoptotic 72 hrs after treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1001753-24-7
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Appearance Solid
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Peso molecular 322.42
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Fòrmula C19H18N2OS
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Color White to off-white
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SMILES
O=C(NC1=NC(C2=C(C)C=C(C)C=C2C)=CS1)C3=CC=CC=C3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
DMSO : 50 mg/mL (155.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Standard XTT assays with a four-day drug treatment procedure were performed to measure the dose-dependent cytotoxicity of INH analogs in cultured cells. Triplicate sets were measured and compiled for final data presentation. Cells were plated on 96-well dishes one day before the drug treatment, followed by drug treatment (2.5 μM INH6) on day 2 and XTT assay on day 5 after drug addition. The absorption at 595 nm was measured with a plate reader and converted to cell survival percentages in comparison to mock treated groups[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1015 mL | 15.5077 mL | 31.0154 mL | 77.5386 mL |
| 5 mM | 0.6203 mL | 3.1015 mL | 6.2031 mL | 15.5077 mL | |
| 10 mM | 0.3102 mL | 1.5508 mL | 3.1015 mL | 7.7539 mL | |
| 15 mM | 0.2068 mL | 1.0338 mL | 2.0677 mL | 5.1692 mL | |
| 20 mM | 0.1551 mL | 0.7754 mL | 1.5508 mL | 3.8769 mL | |
| 25 mM | 0.1241 mL | 0.6203 mL | 1.2406 mL | 3.1015 mL | |
| 30 mM | 0.1034 mL | 0.5169 mL | 1.0338 mL | 2.5846 mL | |
| 40 mM | 0.0775 mL | 0.3877 mL | 0.7754 mL | 1.9385 mL | |
| 50 mM | 0.0620 mL | 0.3102 mL | 0.6203 mL | 1.5508 mL | |
| 60 mM | 0.0517 mL | 0.2585 mL | 0.5169 mL | 1.2923 mL | |
| 80 mM | 0.0388 mL | 0.1938 mL | 0.3877 mL | 0.9692 mL | |
| 100 mM | 0.0310 mL | 0.1551 mL | 0.3102 mL | 0.7754 mL |
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