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Referencia número: HY-W714212

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Fluchloralin

Fluchloralin is a dinitroaniline herbicide that effectively controls annual gramineous and broadleaf weeds primarily by inhibiting tubulin synthesis and cell division. Fluchloralin exhibits cytotoxicity and genotoxicity, and promotes cell apoptosis by activating apoptotic signaling proteins, forming DNA ladder bands, inducing cell shrinkage and nuclear fragmentation.

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No. CAS: 33245-39-5
Fòrmula: C₁₂H₁₃ClF₃N₃O₄
Peso molecular:355.70

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

Fluchloralin Chemical Structure

No. CAS: 33245-39-5

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Descripciòn

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Cellular Effect

Cell Line	Type	Value	Description	References
HFF	IC₅₀	0.33 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	0.77 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235V mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235V mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	1 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235L mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235L mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	1.6 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin H28Q mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin H28Q mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	1.9 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin S6I mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin S6I mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	17.7 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243S mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243S mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	2.1 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235T mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235T mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	2.2 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243C mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243C mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	20.5 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin T239I mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin T239I mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	22.2 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin L136F mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin L136F mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	3.2 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin F24H mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin F24H mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	8 μM Compound: 6	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin V4L mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin V4L mutation infected in HFF cells by plaque assay	[PMID: 20145086]

In Vitro

Fluchloralin (5-20 μg/mL; 24-72 h) exhibits dose- and time-dependent cytotoxicity in CHO and MEF cells^[2].
Fluchloralin (2.5-20.0 μg/mL; 4-12 h) induces statistically significant, time-dependent chromosomal aberrations in CHO cells^[2].
Fluchloralin (1.0-20.0 μg/mL; 12 h) causes a statistically significant increase in the level of sister chromatid exchange in CHO cells^[2].
Fluchloralin (5-20 μg/mL; 30 h) exerts a dose-dependent inhibitory effect on DNA synthesis in synchronized MEF cells without affecting thymidine transport^[2].
Fluchloralin (5-20 μg/mL; 6 h) reduces the DNA synthesis level of synchronized MEF cells by nearly 50% at 12 h post-treatment, without affecting thymidine transport^[2].
Fluchloralin (5-20 μg/mL; 24-72 h) induces apoptosis in CHO cells, which is evidenced by the formation of DNA ladder bands and typical nuclear changes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

Chinese hamster ovary (CHO) cells, mouse embryo fibroblast (MEF) cells
Concentration:

5, 10, 15 and 20 μg/mL
Incubation Time:

24 h; 48 h; 72 h
Result:

Induced a dose-dependent decrease in cell viability in both cell lines, with greater toxicity observed in CHO cells than MEF cells.
Reduced viability to 50% in CHO cells treated with 20 μg/mL for 72 h.
Reduced viability to 50% in MEF cells treated with 20 μg/mL for 72 h.

Apoptosis Analysis^[2]

Cell Line:

Chinese hamster ovary (CHO) cells
Concentration:

5, 10 and 20 μg/mL
Incubation Time:

24 h; 48 h; 72 h
Result:

Induced a characteristic DNA ladder pattern in agarose gels of DNA from treated CHO cells.
Revealed apoptotic features including cell shrinkage and nuclear fragmentation via microscopic observation.
Induced apoptosis at 20 μg/mL after 72 h of treatment.

In Vivo

Fluchloralin (0.5-2 mg/L; aquatic immersion; daily; 96 hours) induces dose-dependent developmental toxicity in Danio rerio embryos, including reduced survival (LC₅₀ = 2.004 mg/L), morphological defects, neurotoxicity, cardiotoxicity, liver injury, and increased brain apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Transgenic zebrafish (olig2:dsRed, cmlc2:dsRed, lfabp:dsRed;elastase:GFP); wild-type zebrafish (reared at 26-28.5 °C on a 13 h light/11 h dark cycle, 8 hours post-fertilization)^[1]
Dosage:

0.5 mg/L; 1 mg/L; 2 mg/L
Administration:

aquatic immersion; daily; 96 hours
Result:

Significantly increased the number of apoptotic cells in the brain region of zebrafish.
Caused developmental toxicity to multiple organs (nervous system, heart, liver, pancreas) in zebrafish embryos.

Chemical Information

No. CAS 33245-39-5
Peso molecular 355.70
Fòrmula C₁₂H₁₃ClF₃N₃O₄
SMILES

ClCCN(CCC)C1=C(C=C(C(F)(F)F)C=C1[N+]([O-])=O)[N+]([O-])=O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureza y Documentación

Instrucciones de manejo (2659 KB)

Referencias

[1]. Hong T, et al. Fluchloralin induces developmental toxicity in heart, liver, and nervous system during early zebrafish embryogenesis. Comp Biochem Physiol C Toxicol Pharmacol. 2023;271:109679. [Content Brief]

[2]. Sinha S, et al. Fluchloralin is cytotoxic and genotoxic and induces apoptosis in mammalian cells. Environ Mol Mutagen. 1998;31(3):257-262. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Fluchloralin

Ver todos los productos específicos de isoformas DNA/RNA Synthesis

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Fluchloralin Related Classifications

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