Maltobionic acid
Based on 1 Customer Validation
Maltobionic acid (4-O-α-D-Glucopyranosyl-D-gluconic acid) is an orally active oligosaccharide and iron chelator with antibacterial activity. Maltobionic acid inhibits the expression of NFATc1, suppresses osteoclast differentiation, inhibits bone resorption, and increases serum calcitonin levels. Maltobionic acid protects mammalian cells from hydrogen peroxide (H2O2)-induced oxidative damage; resists fermentation by the gut microbiota; and exhibits anti-digestive and anti-fermentative properties. Maltobionic acid can be used in research related to osteoporosis, bacterial infections, and constipation.
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- Pureza: 90.0%
- No. CAS: 534-42-9
- Fòrmula: C12H22O12
- Peso molecular:358.30
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Maltobionic acid (0.01-1000 μM; 72 h) significantly inhibits sRANKL (an NF-κB activator)-induced osteoclast differentiation of RAW264 cells without reducing cell viability[1].
Maltobionic acid (100-1000 μM; 48 h) significantly reduces the expression of NFATc1, a key transcription factor for osteoclast differentiation, in sRANKL-stimulated RAW264 cells[1].
Maltobionic acid (100-1000 μM; 15 min) significantly enhances sRANKL-induced IκBα phosphorylation in RAW264 cells[1].
Maltobionic acid (0.4-50 mg/mL; 24 h) inhibits the growth of *Salmonella choleraesuis*, *Escherichia coli*, *Staphylococcus aureus* and *Listeria monocytogenes*, with MIC values of 8.0, 8.5, 10.5 and 8.0 mg/mL, respectively[2].
Maltobionic acid (100 μg/mL; 24.5 h) exerts cytoprotective effects on HEK-293 cells against H2O2-induced oxidative damage[2].
Cassava starch-chitosan films supplemented with 7.5% (w/w) maltose acid inhibit the growth of *Listeria monocytogenes*, *Salmonella choleraesuis*, *Escherichia coli* and *Staphylococcus aureus*, with a zone of inhibition diameter ranging from 15.67 to 22.33 mm[2].
Sodium maltobionate (10% (w/v) to 1% (w/w); 30 min-6 h) is completely indigestible by artificial saliva, gastric juice, and pancreatic juice in vitro, and is only minimally (3.3%) digested by rat intestinal enzymes[4].
Sodium maltobionate (0.5% (w/v); 3 days) is selectively utilized by specific human intestinal bacteria, and exhibits strong proliferative activity against Bifidobacterium dentium and Bifidobacterium adolescentis[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:murine macrophage RAW264 cells
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Concentration:0.01, 0.1, 1, 10, 100 and 1000 μM
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Incubation Time:72 h
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Result:Caused a significant decrease in the number of multinucleated (4+ nuclei) tartrate-resistant acid phosphatase-positive osteoclast-like cells relative to control.
Did not significantly affect cell viability at any tested concentration.
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Cell Line:murine macrophage RAW264 cells
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Concentration:0.01, 0.1, 1, 10, 100 and 1000 μM
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Incubation Time:48 h
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Result:Caused a significant decrease in NFATc1 protein expression relative to the sRANKL-treated control.
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Cell Line:murine macrophage RAW264 cells
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Concentration:0.01, 0.1, 1, 10, 100 and 1000 μM
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Incubation Time:15 min
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Result:Caused a significant increase in phosphorylated IκBα protein levels relative to the sRANKL-treated control.
Maltobionic acid (1600 mg/kg; p.o.; single administration) exhibits high resistance to digestion and fermentation in rats[3].
Maltobionic acid enhances intestinal absorption of Ca2+ and Mg2+ in healthy rats, and increases the weight of cecal contents and levels of short-chain fatty acids[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ddY mice (4-week-old female; ovariectomized)[1]
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Dosage:5.0% (w/w)
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Administration:p.o.; ad libitum; 80 days
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Result:Increased femur dry weight to 60.8 mg (vs. 52.4 mg in control).\nIncreased femur calcium content to 12.7 mg (vs. 10.2 mg in control).
Increased femur phosphorus content to 5.29 mg (vs. 4.12 mg in control).
Increased serum calcitonin levels significantly.
Decreased serum tartrate-resistant acid phosphatase 5b (TRACP-5b) levels significantly.
Showed an upward trend in femoral bone mineral density.
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Animal Model:Wistar rats (male, initial body weight 220 g)[3]
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Dosage:1600 mg/kg
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Administration:p.o.; single dose
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Result:Was minimally hydrolyzed by brush border membrane vesicles (BBMV).
Produced no significant difference in exhaled hydrogen levels over 8 h following administration compared with controls.
Chemical Information
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No. CAS 534-42-9
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Appearance Solid
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Peso molecular 358.30
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Fòrmula C12H22O12
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Color White to off-white
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SMILES
OC[C@H]([C@H]([C@@H]([C@H]1O)O)O)O[C@@H]1O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C(O)=O
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Synonyms
4-O-α-D-Glucopyranosyl-D-gluconic acid
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (279.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[2]. de Souza RC, et al. Purification, bioactivity and application of maltobionic acid in active films. 3 Biotech. 2024;14(1):32. [Content Brief]
[3]. Tanabe K, et al. Metabolic fate of newly developed nondigestible oligosaccharide, maltobionic acid, in rats and humans. Food Sci Nutr. 2020;8(7):3610-3616. Published 2020 May 20. [Content Brief]
[4]. Fukami K, et al. In Vitro Utilization Characteristics of Maltobionic Acid and Its Effects on Bowel Movements in Healthy Subjects. J Appl Glycosci (1999). 2020;67(1):1-9. Published 2020 Feb 20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7910 mL | 13.9548 mL | 27.9096 mL | 69.7739 mL |
| 5 mM | 0.5582 mL | 2.7910 mL | 5.5819 mL | 13.9548 mL | |
| 10 mM | 0.2791 mL | 1.3955 mL | 2.7910 mL | 6.9774 mL | |
| 15 mM | 0.1861 mL | 0.9303 mL | 1.8606 mL | 4.6516 mL | |
| 20 mM | 0.1395 mL | 0.6977 mL | 1.3955 mL | 3.4887 mL | |
| 25 mM | 0.1116 mL | 0.5582 mL | 1.1164 mL | 2.7910 mL | |
| 30 mM | 0.0930 mL | 0.4652 mL | 0.9303 mL | 2.3258 mL | |
| 40 mM | 0.0698 mL | 0.3489 mL | 0.6977 mL | 1.7443 mL | |
| 50 mM | 0.0558 mL | 0.2791 mL | 0.5582 mL | 1.3955 mL | |
| 60 mM | 0.0465 mL | 0.2326 mL | 0.4652 mL | 1.1629 mL | |
| 80 mM | 0.0349 mL | 0.1744 mL | 0.3489 mL | 0.8722 mL | |
| 100 mM | 0.0279 mL | 0.1395 mL | 0.2791 mL | 0.6977 mL |
Maltobionic acid Related Classifications
HY-W423191 Related Classifications
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- Maltobionic acid
- 534-42-9
- 4-O-α-D-Glucopyranosyl-D-gluconic acid
- Nuclear Factor of activated T Cells (NFAT)
- Bacterial
- nuclear factor of activated T-cell cytoplasmic 1
- bone resorption
- osteoclast differentiation
- ovariectomy-induced femoral bone mineral content
- HEK-293 cells
- Salmonella enterica serovar Choleraesuis
- Escherichia coli
- RAW264 cells
- serum calcitonin
- ovariectomy-induced femoral bone mineral density
- Inhibitor
- inhibitor
- inhibit