NC03
Based on 1 Customer Validation
NC03 is a PI4K2A inhibitor and reducing agent. NC03 inhibits the activity of PI4K2A, thereby reducing the production of PI4P in the cellular compartments where PI4K2A functions. NC03 rapidly induces a decrease in PI4P pools in the Golgi and endosomal compartments. NC03 reduces PI4P production in Rab7-positive late endosomes.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.79%
- No. CAS: 568558-66-7
- Fòrmula: C21H21N3O7S
- Peso molecular:459.47
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
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Actividad biológica
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PI4KIIα |
NC03 (15-76 μM; 1 hour) potently inhibits the activity of bacterially expressed human PI4K2A enzyme in COS-7 cells[1].
NC03 (10 μM; 10 min pre-incubation, 1.5 h kinase reaction) inhibits the activity of palmitoylated PI4K2A enzyme isolated from COS-7 cells when used at 10 μM[1].
NC03 (1-10 μM; 20 min pre-incubation, 30 min permeabilized cell incubation) inhibits PI4K2A-mediated PI4P synthesis in permeabilized COS-7 cells, with secondary effects on PI(4,5)P2 levels[1].
NC03 (10 μM; up to 10 min) rapidly reduces Golgi and endosomal PI4P pools in live COS-7 cells when used at 10 μM[1].
NC03 (10-30 μM; 30 min) decreases Rab7-positive late endosomal PI4P levels in intact HEK-AT1 cells in a concentration-dependent manner, with 30 μM showing greater efficacy than 10 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:COS-7
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Concentration:15, 76 μM
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Incubation Time:1 h
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Result:Inhibited the activity of bacterially expressed human PI4K2A enzyme, with efficacy that correlates with activity against palmitoylated PI4K2A from COS-7 cells.
Chemical Information
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No. CAS 568558-66-7
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Appearance Solid
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Peso molecular 459.47
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Fòrmula C21H21N3O7S
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Color White to off-white
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SMILES
O=C(CSC1=NN=C(C2=CC(OC)=C(OC)C(OC)=C2)O1)NC3=CC=C4OCCOC4=C3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (217.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1764 mL | 10.8821 mL | 21.7642 mL | 54.4105 mL |
| 5 mM | 0.4353 mL | 2.1764 mL | 4.3528 mL | 10.8821 mL | |
| 10 mM | 0.2176 mL | 1.0882 mL | 2.1764 mL | 5.4411 mL | |
| 15 mM | 0.1451 mL | 0.7255 mL | 1.4509 mL | 3.6274 mL | |
| 20 mM | 0.1088 mL | 0.5441 mL | 1.0882 mL | 2.7205 mL | |
| 25 mM | 0.0871 mL | 0.4353 mL | 0.8706 mL | 2.1764 mL | |
| 30 mM | 0.0725 mL | 0.3627 mL | 0.7255 mL | 1.8137 mL | |
| 40 mM | 0.0544 mL | 0.2721 mL | 0.5441 mL | 1.3603 mL | |
| 50 mM | 0.0435 mL | 0.2176 mL | 0.4353 mL | 1.0882 mL | |
| 60 mM | 0.0363 mL | 0.1814 mL | 0.3627 mL | 0.9068 mL | |
| 80 mM | 0.0272 mL | 0.1360 mL | 0.2721 mL | 0.6801 mL | |
| 100 mM | 0.0218 mL | 0.1088 mL | 0.2176 mL | 0.5441 mL |
NC03 Related Classifications
HY-115675 Related Classifications
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