Smad3 - SMAD family member 3 Gene

Also Known as Madh3

Species: Mus musculus

Gene Type: protein coding
Gene ID: 17127

Summary

Enables several functions, including DNA binding activity; DNA-binding transcription activator activity, RNA polymerase II-specific; and promoter-specific chromatin binding activity. Involved in several processes, including JNK cascade; negative regulation of osteoblast proliferation; and positive regulation of chondrocyte differentiation. Acts upstream of or within several processes, including animal organ development; regulation of transcription by RNA polymerase II; and transmembrane receptor protein serine/threonine kinase signaling pathway. Located in cytoplasm; nucleus; and plasma membrane. Is expressed in several structures, including alimentary system; early conceptus; genitourinary system; limb; and nervous system. Used to study Osteoarthritis. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 3; Lynch syndrome; and Pancreatic Cancer. Orthologous to human SMAD3 (Smad Family member 3). [provided by Alliance of Genome Resources, Apr 2022]

Smad3 Products (1)

mRNA Protein Name
NM_016769.4 NP_058049.3 mothers against decapentaplegic homolog 3

Gene Ontology

  • Molecular Function
  • Biological Process
  • Cellular Component
Molecular Function GO Annotation Evidence Références Source
enables DNA binding IDA
IDA: Inferred from direct assay
27336669 MGI
enables DNA-binding transcription activator activity, RNA polymerase II-specific IPI
IPI: Inferred from physical interaction
24964035 MGI
enables DNA-binding transcription factor activity IDA
IDA: Inferred from direct assay
12943993 MGI
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding IDA
IDA: Inferred from direct assay
25065622 MGI
enables RNA polymerase II-specific DNA-binding transcription factor binding IPI
IPI: Inferred from physical interaction
11937490 MGI
enables chromatin DNA binding IDA
IDA: Inferred from direct assay
16224064 MGI
enables chromatin binding IDA
IDA: Inferred from direct assay
16619037 MGI
enables collagen binding IPI
IPI: Inferred from physical interaction
14559231 MGI
enables double-stranded DNA binding IDA
IDA: Inferred from direct assay
15282343 MGI
enables identical protein binding IPI
IPI: Inferred from physical interaction
21724602 MGI
enables promoter-specific chromatin binding IDA
IDA: Inferred from direct assay
18802055 MGI
enables protein binding IPI
IPI: Inferred from physical interaction
11711431 MGI
Biological Process GO Annotation Evidence Références Source
involved in JNK cascade IMP
IMP: Inferred from mutant phenotype
18802055 MGI
involved in MAPK cascade IMP
IMP: Inferred from mutant phenotype
18802055 MGI
acts upstream of or within SMAD protein signal transduction IGI
IGI: Inferred from genetic interaction
23390484 MGI
involved in T cell activation IMP
IMP: Inferred from mutant phenotype
10064594 MGI
acts upstream of or within apoptotic process IMP
IMP: Inferred from mutant phenotype
19679673 MGI
acts upstream of or within cell population proliferation IGI
IGI: Inferred from genetic interaction
19679673 MGI
involved in cellular response to virus IDA
IDA: Inferred from direct assay
18802055 MGI
acts upstream of or within developmental growth IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within embryonic cranial skeleton morphogenesis IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within embryonic foregut morphogenesis IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within embryonic pattern specification IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within endoderm development IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within gastrulation IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within heart looping IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within immune system development IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within in utero embryonic development IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within lens fiber cell differentiation IMP
IMP: Inferred from mutant phenotype
17215516 MGI
acts upstream of or within liver development IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within mesoderm formation IMP
IMP: Inferred from mutant phenotype
15084457 MGI
involved in negative regulation of inflammatory response IMP
IMP: Inferred from mutant phenotype
10559937 MGI
acts upstream of or within negative regulation of osteoblast differentiation IGI
IGI: Inferred from genetic interaction
15150273 MGI
involved in negative regulation of osteoblast proliferation IMP
IMP: Inferred from mutant phenotype
21035443 MGI
acts upstream of or within negative regulation of transcription by RNA polymerase II IDA
IDA: Inferred from direct assay
9702197 MGI
involved in negative regulation of wound healing IMP
IMP: Inferred from mutant phenotype
10559937 MGI
acts upstream of or within osteoblast development IGI
IGI: Inferred from genetic interaction
15150273 MGI
involved in osteoblast differentiation IMP
IMP: Inferred from mutant phenotype
11585338 MGI
acts upstream of or within paraxial mesoderm morphogenesis IMP
IMP: Inferred from mutant phenotype
15084457 MGI
acts upstream of or within pericardium development IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within positive regulation of DNA-templated transcription IDA
IDA: Inferred from direct assay
15282343 MGI
involved in positive regulation of chondrocyte differentiation IMP
IMP: Inferred from mutant phenotype
21035443 MGI
acts upstream of or within positive regulation of transcription by RNA polymerase II IDA
IDA: Inferred from direct assay
12943993 MGI
involved in positive regulation of transcription by RNA polymerase II IDA
IDA: Inferred from direct assay
25065622 MGI
acts upstream of or within positive regulation of transcription by RNA polymerase II IGI
IGI: Inferred from genetic interaction
15150273 MGI
involved in positive regulation of transcription by RNA polymerase II IMP
IMP: Inferred from mutant phenotype
18802055 MGI
acts upstream of or within regulation of binding IDA
IDA: Inferred from direct assay
15282343 MGI
acts upstream of or within regulation of epithelial cell proliferation IMP
IMP: Inferred from mutant phenotype
12845704 MGI
involved in regulation of immune response IMP
IMP: Inferred from mutant phenotype
10064594 MGI
acts upstream of or within regulation of striated muscle tissue development IDA
IDA: Inferred from direct assay
11711431 MGI
acts upstream of or within regulation of transforming growth factor beta receptor signaling pathway IDA
IDA: Inferred from direct assay
11711431 MGI
acts upstream of or within response to transforming growth factor beta IGI
IGI: Inferred from genetic interaction
19679673 MGI
acts upstream of or within skeletal system development IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within somitogenesis IMP
IMP: Inferred from mutant phenotype
15183723 MGI
acts upstream of or within thyroid gland development IGI
IGI: Inferred from genetic interaction
15183723 MGI
acts upstream of or within transforming growth factor beta receptor signaling pathway IDA
IDA: Inferred from direct assay
15239668 MGI
acts upstream of or within transforming growth factor beta receptor signaling pathway IMP
IMP: Inferred from mutant phenotype
15239668 MGI
involved in ureteric bud development IEP
IEP: Inferred from expression pattern
14656760 MGI
Cellular Component GO Annotation Evidence Références Source
located in cytoplasm IDA
IDA: Inferred from direct assay
11160896 MGI
located in nucleus IDA
IDA: Inferred from direct assay
11160896 MGI
located in plasma membrane IDA
IDA: Inferred from direct assay
12543979 MGI
EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern
Protein Preferred Names Protein Names

mothers against decapentaplegic homolog 3

  • MAD homolog 3

  • Smad 3

  • mMad3

  • mad3

  • mothers against DPP homolog 3

Smad3 Protein-protein interaction Information

Type
Protein Name Protein ID Interactor Interactor Species Interactor ID Detection Method Références
Intra
Smad3 Q8BUN5 Pou5f1 Mus musculus P20263
Anti Bait CoIP
22036565
Intra
Smad3 Q8BUN5 Pou5f1 Mus musculus P20263
CH-IP
22036565
Intra
Smad3 Q8BUN5 Pou5f1 Mus musculus P20263
Crosslink
22036565
Intra
Smad3 Q8BUN5 Axin1 Mus musculus O35625
Anti Tag CoIP
16601693
Intra
Smad3 Q8BUN5 Smad4 Mus musculus P97471
Anti Bait CoIP
18268325
Intra
Smad3 Q8BUN5 Smad4 Mus musculus P97471
Anti Bait CoIP
22045334
Intra
Smad3 Q8BUN5 Smad4 Mus musculus P97471
Anti Bait CoIP
22036565
Intra
Smad3 Q8BUN5 Smad4 Mus musculus P97471
PLA
22442258
Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant Smad3 Proteins

Cat. No. Nom du produit Accession Pureté
HY-P73636 SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST, solution) P84022-1/Q8BUN5/P84025 (M1-S425) ≥ 85%, as determined by reducing SDS-PAGE.

Related Agents

  • Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)
Cat. No. Nom du produit
Effect
Pureté
Rare Diseases
HY-147025 (S,R,S)-AHPC-C2-amide-benzofuranylmethyl-pyridine Inhibitor 98.62% Unkown
HY-187219 CX-Se13 Inhibitor / Unkown
HY-P11914 Peptide CYP9 Inhibitor / Unkown

Orthologs Information

Species Symbol Source ID
Homo sapiens Smad3 NCBI NCBI:4088