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Pou5f1 - POU domain, class 5, transcription factor 1 Gene

Also Known as Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7

Species: Mus musculus

Gene Type: protein coding

Gene ID: 18999

About Pou5f1

Chromosome 17: 35,816,915-35,821,669 forward strand.GRCm39:CM001010.3

This gene has 6 transcripts (splice variants), 331 orthologues, 16 paralogues and is associated with 4 phenotypes. Biased expression in testis adult (RPKM 4.) and ovary adult (RPKM 1.5).

Summary

The protein encoded by this gene belongs to the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein plays a key role in embryonic development and stem cell pluripotency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Pou5f1 Products (2)

mRNA	Protein	Name
NM_013633.3	NP_038661.2	POU domain, class 5, transcription factor 1 isoform 1
NM_001252452.1	NP_001239381.1	POU domain, class 5, transcription factor 1 isoform 2

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables DNA binding	IDA IDA: Inferred from direct assay	1967980	MGI
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA IDA: Inferred from direct assay	12615074	MGI
enables DNA-binding transcription factor activity	IDA IDA: Inferred from direct assay	1967980	MGI
enables DNA-binding transcription factor activity	IMP IMP: Inferred from mutant phenotype	23376973	MGI
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA IDA: Inferred from direct assay	15047715	MGI
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IMP IMP: Inferred from mutant phenotype	29153991	MGI
enables DNA-binding transcription factor binding	IPI IPI: Inferred from physical interaction	17541407	MGI
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA IDA: Inferred from direct assay	12615074	MGI
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IGI IGI: Inferred from genetic interaction	15863505	MGI
enables RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding	IDA IDA: Inferred from direct assay	17938240	MGI
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI IPI: Inferred from physical interaction	18662995	MGI
enables chromatin DNA binding	IDA IDA: Inferred from direct assay	24268575	MGI
enables chromatin binding	IDA IDA: Inferred from direct assay	15988017	MGI
enables cis-regulatory region sequence-specific DNA binding	IDA IDA: Inferred from direct assay	23495099	MGI
enables cytokine binding	IDA IDA: Inferred from direct assay	23495099	MGI
enables lysine-acetylated histone binding	IDA IDA: Inferred from direct assay	23798425	MGI
enables protein binding	IPI IPI: Inferred from physical interaction	7590241	MGI
enables sequence-specific DNA binding	IDA IDA: Inferred from direct assay	1690859	MGI
enables transcription cis-regulatory region binding	IDA IDA: Inferred from direct assay	17541407	MGI
enables ubiquitin protein ligase binding	IPI IPI: Inferred from physical interaction	15047715	MGI

Biological Process GO Annotation	Evidence	References	Source
involved in blastocyst development	IEP IEP: Inferred from expression pattern	21258368	MGI
acts upstream of or within blastocyst growth	IGI IGI: Inferred from genetic interaction	24036311	MGI
acts upstream of or within cell fate commitment	IMP IMP: Inferred from mutant phenotype	9814708	MGI
acts upstream of or within cellular response to leukemia inhibitory factor	IEP IEP: Inferred from expression pattern	20439489	MGI
acts upstream of or within ectodermal cell fate commitment	IDA IDA: Inferred from direct assay	10742100	MGI
acts upstream of or within endodermal cell fate commitment	IDA IDA: Inferred from direct assay	10742100	MGI
involved in gene expression	IMP IMP: Inferred from mutant phenotype	17267691	MGI
acts upstream of or within germ-line stem cell population maintenance	IMP IMP: Inferred from mutant phenotype	15486564	MGI
acts upstream of or within mesodermal cell fate commitment	IDA IDA: Inferred from direct assay	10742100	MGI
acts upstream of or within negative regulation of DNA-templated transcription	IDA IDA: Inferred from direct assay	1967980	MGI
acts upstream of or within negative regulation of calcium ion-dependent exocytosis	IDA IDA: Inferred from direct assay	2357966	MGI
acts upstream of or within negative regulation of cell differentiation	IGI IGI: Inferred from genetic interaction	16325584	MGI
involved in negative regulation of gene expression	IDA IDA: Inferred from direct assay	23495099	MGI
acts upstream of or within negative regulation of transcription by RNA polymerase II	IGI IGI: Inferred from genetic interaction	16325584	MGI
acts upstream of or within positive regulation of DNA-templated transcription	IDA IDA: Inferred from direct assay	1967980	MGI
acts upstream of or within positive regulation of gene expression	IDA IDA: Inferred from direct assay	17938240	MGI
involved in positive regulation of gene expression	IDA IDA: Inferred from direct assay	23495099	MGI
acts upstream of or within positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	1972777	MGI
involved in positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	12615074	MGI
acts upstream of or within positive regulation of transcription by RNA polymerase II	IGI IGI: Inferred from genetic interaction	7590241	MGI
acts upstream of or within positive regulation of transcription by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	15988017	MGI
involved in positive regulation of transcription by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	23376973	MGI
involved in positive regulation of transcription initiation by RNA polymerase II	IDA IDA: Inferred from direct assay	26691508	MGI
acts upstream of or within regulation of DNA-templated transcription	IDA IDA: Inferred from direct assay	12665572	MGI
involved in regulation of asymmetric cell division	IEP IEP: Inferred from expression pattern	21258368	MGI
involved in regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	15047715	MGI
acts upstream of or within response to retinoic acid	IDA IDA: Inferred from direct assay	18400104	MGI
acts upstream of or within somatic stem cell population maintenance	IMP IMP: Inferred from mutant phenotype	9814708	MGI
acts upstream of or within stem cell differentiation	IDA IDA: Inferred from direct assay	20123909	MGI
involved in stem cell differentiation	IMP IMP: Inferred from mutant phenotype	23376973	MGI
involved in stem cell population maintenance	IMP IMP: Inferred from mutant phenotype	23376973	MGI
acts upstream of or within transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	1967980	MGI
acts upstream of trophectodermal cell differentiation	IGI IGI: Inferred from genetic interaction	16325584	MGI
acts upstream of or within trophectodermal cell differentiation	IGI IGI: Inferred from genetic interaction	16325584	MGI
acts upstream of or within trophectodermal cell fate commitment	IDA IDA: Inferred from direct assay	10742100	MGI

Cellular Component GO Annotation	Evidence	References	Source
is active in chromatin	IDA IDA: Inferred from direct assay	23798425	MGI
located in chromatin	IDA IDA: Inferred from direct assay	23495099	MGI
located in cytoplasm	IDA IDA: Inferred from direct assay	7958450	MGI
located in cytoplasm	IMP IMP: Inferred from mutant phenotype	29153991	MGI
located in female germ cell nucleus	IDA IDA: Inferred from direct assay	7958450	MGI
located in germ cell nucleus	IDA IDA: Inferred from direct assay	12641567	MGI
located in male germ cell nucleus	IDA IDA: Inferred from direct assay	9507072	MGI
located in nucleolus	IDA IDA: Inferred from direct assay	19480014	MGI
located in nucleoplasm	IDA IDA: Inferred from direct assay	21076177	MGI
is active in nucleus	IDA IDA: Inferred from direct assay	23798425	MGI
located in nucleus	IDA IDA: Inferred from direct assay	7958450	MGI
located in nucleus	IMP IMP: Inferred from mutant phenotype	23376973	MGI
part of transcription regulator complex	IDA IDA: Inferred from direct assay	7969117	MGI
part of transcription repressor complex	IDA IDA: Inferred from direct assay	16325584	MGI

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Pou5f1 Protein Structure

POU

Homeobox

0
100
200
300
352 a.a.

Protein Preferred Names	Protein Names
POU domain, class 5, transcription factor 1	octamer-binding protein 3 octamer-binding protein 4 octamer-binding transcription factor 3 octamer-binding transcription factor 4 alternative

Pou5f1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	Pou5f1	P20263	Wdr5	Mus musculus	P61965	Anti Tag CoIP	20362541
Intra	Pou5f1	P20263	Wdr5	Mus musculus	P61965	Anti Bait CoIP	20362541
Intra	Pou5f1	P20263	Wdr5	Mus musculus	P61965	Anti Tag CoIP	21477851
Intra	Pou5f1	P20263	Wdr5	Mus musculus	P61965	Anti Bait CoIP	21477851
Intra	Pou5f1	P20263	Ewsr1	Mus musculus	Q61545	Pull Down	15917470
Intra	Pou5f1	P20263	Ewsr1	Mus musculus	Q61545	Y2H	15917470
Intra	Pou5f1	P20263	Ewsr1	Mus musculus	Q61545	Anti Bait CoIP	15917470
Intra	Pou5f1	P20263	Nanog	Mus musculus	Q80Z64	Pull Down	17093407
Intra	Pou5f1	P20263	Nanog	Mus musculus	Q80Z64	Anti Tag CoIP	17093407
Intra	Pou5f1	P20263	Nr0b1	Mus musculus	Q61066	Anti Tag CoIP	20362541
Intra	Pou5f1	P20263	Sox2	Mus musculus	P48432	Pull Down	23108051
Intra	Pou5f1	P20263	Sox2	Mus musculus	P48432	Anti Tag CoIP	20362541
Intra	Pou5f1	P20263	Parp1	Mus musculus	P11103	TAP	20362542
Intra	Pou5f1	P20263	Pkm	Mus musculus	P52480	Anti Tag CoIP	18191611
Intra	Pou5f1	P20263	Cdk1	Mus musculus	P11440	Pull Down	23108051
Intra	Pou5f1	P20263	Mta2	Mus musculus	Q9R190	TAP	20362542
Intra	Pou5f1	P20263	Mta2	Mus musculus	Q9R190	Anti Tag CoIP	20362541
Intra	Pou5f1	P20263	Mta2	Mus musculus	Q9R190	Anti Bait CoIP	20362541

Cross: Cross-species interaction Intra: Intraspecies interaction

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (6)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS10907	POU5F1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS10908	Pou5f1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-168566	EV206	Inhibitor	99.62%	Unkown
HY-RS10909	Pou5f1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS10910	POU5F1B Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-174544	Human POU5F1 mRNA		/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Homo sapiens	Pou5f1	NCBI	NCBI:5460