2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. TGF-beta Superfamily
  5. Smad Family
  6. SMAD3
  7. SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST)

SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST)

Cat. No.: HY-P73636
Handling Instructions

The SMAD3 protein is an R-SMAD that acts as an intracellular signal transducer activated by TGF-β and activin type 1 receptor kinase. It binds TRE elements and regulates TGF-β target genes. SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST) is the recombinant rat-derived SMAD3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST) is 425 a.a., with molecular weight of ~75.9 kDa.

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Description

The SMAD3 protein is an R-SMAD that acts as an intracellular signal transducer activated by TGF-β and activin type 1 receptor kinase. It binds TRE elements and regulates TGF-β target genes. SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST) is the recombinant rat-derived SMAD3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST) is 425 a.a., with molecular weight of ~75.9 kDa.
Background

The SMAD3 protein serves as a receptor-regulated SMAD (R-SMAD), functioning as an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. It binds the TRE element in the promoter region of genes regulated by TGF-beta, and upon forming the SMAD3/SMAD4 complex, activates transcription. Additionally, SMAD3 can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. This protein plays a role in wound healing, potentially modulating the growth and migration of primary keratinocytes and altering TGF-mediated chemotaxis of monocytes, with its impact on wound healing being hormone-sensitive. SMAD3 also acts as a regulator of chondrogenesis and osteogenesis and inhibits the early healing of bone fractures. Furthermore, it positively regulates PDPK1 kinase activity by stimulating its dissociation from the negative regulator 14-3-3 protein YWHAQ. The protein exists as a monomer in the absence of TGF-beta and forms a homooligomer in its presence, or a heterotrimer with C-terminally phosphorylated SMAD2 or SMAD3 and SMAD4 to form the transcriptionally active SMAD2/SMAD3-SMAD4 complex. SMAD3 interacts with various proteins involved in diverse cellular processes, including signal transduction, transcriptional regulation, ubiquitination, and protein-protein interactions.
Species

Rat
Source

Sf9 insect cells
Tag

N-His;N-GST
Accession

Q8BUN5 (M1-S425)
Gene ID

17127  [NCBI]
Molecular Construction
N-term
His-GST
SMAD3 (M1-S425)
Accession # Q8BUN5
C-term
Synonyms
Mothers against decapentaplegic homolog 3; mMad3; Smad3; Madh3
Molecular Weight

Approximately 75.9 kDa
Purity

Greater than 85% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SMAD3 Protein, Human/Mouse/Rat (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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