GL67 pentahydrochloride
Based on 1 Customer Validation
GL67 pentahydrochloride (N4-Spermine cholesteryl carbamate pentahydrochloride) is a cationic lipid that serves as a non-viral siRNA delivery vector and a non-viral gene transfer vector. GL67 pentahydrochloride enhances the encapsulation efficiency of siRNA in liposomal formulations and promotes the cellular uptake of siRNA across cancer cell membranes. GL67 pentahydrochloride mediates the transduction of plasmid DNA into cells. GL67 pentahydrochloride can be used in drug delivery-related research.
For research use only. We do not sell to patients.
- Purity: 99.95%
- Formula: C38H75Cl5N4O2
- Molecular Weight:797.29
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Metabolism/Protease Compound Library
HY-L012
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Lipid Compound Library
HY-L043
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MCE Bioactive Compound Library
HY-L001V
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High-Throughput Bioactive Compound Library
HY-L205
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Liposome Library
HY-L214
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Ionizable Lipid Compound Library
HY-L251
Biological Activity
GL67 pentahydrochloride lipid complexes display enlarged particle sizes and positively shifted zeta potentials as the cationic lipid-to-siRNA molar ratio rises from 1:1 to 3:1, with the 100% GL67 formulation at 3:1 generating a high positive zeta potential of 47 mV. It elevates siRNA encapsulation efficiency across all tested ratios, and complete encapsulation is attained with less GL67 at higher lipid-siRNA ratios[1].
GL67 pentahydrochloride-based lipid complex at a 3:1 cationic lipid/siRNA molar ratio shows favorable metabolic and membrane integrity compatibility with A549 cells and boosts cellular uptake of Cy3-siRNA in A549 cells[1].
GL67 (GL-67) pentahydrochloride mediated markedly lower gene transfection efficiency in polarized differentiated primary human and canine tracheobronchial epithelial cells cultured at the air-liquid interface, along with suppressed cell division and elevated transepithelial electrical resistance. Its transfection capacity dropped with higher cell confluence in plate-grown unpolarized human bronchial epithelial cells and declined in growth-arrested, poorly proliferative primary human bronchial epithelial cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 797.29
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Formula C38H75Cl5N4O2
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Color White to off-white
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SMILES
C[C@@]12[C@]3([H])[C@](CC=C1C[C@H](CC2)OC(N(CCCN)CCCCNCCCN)=O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)CCCC(C)C)C.[H]Cl.[H]Cl.[H]Cl.[H]Cl.[H]Cl
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Synonyms
N4-Spermine cholesteryl carbamate pentahydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 100 mg/mL (125.42 mM)
DMSO : 50 mg/mL (62.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Jarallah SJ, et al. GL67 lipid-based liposomal formulation for efficient siRNA delivery into human lung cancer cells. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society. 2023 Jul;31(7):1139-1148. [Content Brief]
[2]. Jiang C, et al. Efficiency of cationic lipid-mediated transfection of polarized and differentiated airway epithelial cells in vitro and in vivo. Human gene therapy. 1998 Jul 20;9(11):1531-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.2542 mL | 6.2712 mL | 12.5425 mL | 31.3562 mL |
| 5 mM | 0.2508 mL | 1.2542 mL | 2.5085 mL | 6.2712 mL | |
| 10 mM | 0.1254 mL | 0.6271 mL | 1.2542 mL | 3.1356 mL | |
| 15 mM | 0.0836 mL | 0.4181 mL | 0.8362 mL | 2.0904 mL | |
| 20 mM | 0.0627 mL | 0.3136 mL | 0.6271 mL | 1.5678 mL | |
| 25 mM | 0.0502 mL | 0.2508 mL | 0.5017 mL | 1.2542 mL | |
| 30 mM | 0.0418 mL | 0.2090 mL | 0.4181 mL | 1.0452 mL | |
| 40 mM | 0.0314 mL | 0.1568 mL | 0.3136 mL | 0.7839 mL | |
| 50 mM | 0.0251 mL | 0.1254 mL | 0.2508 mL | 0.6271 mL | |
| 60 mM | 0.0209 mL | 0.1045 mL | 0.2090 mL | 0.5226 mL | |
| H2O | 80 mM | 0.0157 mL | 0.0784 mL | 0.1568 mL | 0.3920 mL |
| 100 mM | 0.0125 mL | 0.0627 mL | 0.1254 mL | 0.3136 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
GL67 pentahydrochloride Related Classifications
HY-149037A Related Classifications
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- GL67 pentahydrochloride
- N4-Spermine cholesteryl carbamate pentahydrochloride
- Liposome
- Drug Intermediate
- airway epithelial cells
- siRNA
- chloride transport defects
- transgenic mice
- A549 cells
- cystic fibrosis
- cancer cell membranes
- lung cancer
- normal human bronchial epithelial cells
- canine tracheobronchial epithelial cells
- Inhibitor
- inhibitor
- inhibit