Gly-His-Lys acetate

Cat. No.: HY-W010989 Purity: 99.68%: Data Sheet
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Gly-His-Lys acetate is a natural, circulating regulatory and antimicrobial tripeptide derived from extracellular matrix proteins. Gly-His-Lys acetate binds Cu²⁺ to support copper enzyme activation, antioxidant processes, cellular bioenergetics, and the synthesis of elastin, collagen and catecholamines. Gly-His-Lys acetate regulates cell growth, differentiation and tissue repair, and exerts regenerative, anxiolytic, anti-inflammatory, analgesic and immunosuppressive activities. Gly-His-Lys acetate induces liver degenerative changes. Gly-His-Lys acetate can be used for the research of infections, anxiety, pain-related behaviors and immune-associated liver diseases.

For research use only. We do not sell to patients.

Gly-His-Lys acetate Chemical Structure

CAS No. : 72957-37-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Gly-His-Lys acetate display potent antimicrobial activity against Staphylococcus aureus spp., Escherichia coli spp., Pseudomonas aeruginosa spp. and Candida albicans spp., and exert rapid bactericidal and fungicidal effects^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Gly-His-Lys (0.5-50 μg/kg; i.p.; single dose) acetate produces anxiolytic effects in male Wistar rats^[2].
Gly-His-Lys (5-150 μg/kg; i.p.; single dose, 12 min pre-stimulation) acetate exhibits dose-dependent analgesic and antiaggresogenic effects in rats with pain-induced aggressive-defensive behavior^[3].
Gly-His-Lys (0.5-450 mg/kg; i.p.; daily; 10 days) acetate stimulates hepatocyte mitotic index in a dose-independent manner at ≥ 1.5 mg/kg and exerts a dose-dependent suppression of humoral immune response in CBA mice^[4].
Gly-His-Lys (0.5-450 mg/kg; i.p.; daily; 10 days) acetate exerts a dose-dependent suppression of delayed-type hypersensitivity in Wistar rats at ≥ 1.5 mg/kg^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wistar (male, 180-220 g)^[2]
Dosage:	0.5 μg/kg; 5 μg/kg; 50 μg/kg
Administration:	i.p.; single dose
Result:	Increased time spent in open arms, entries into open arms, and time on the central platform, while decreasing time in closed arms at 0.5 and 5 μg/kg; at 50 μg/kg, it still elevated central platform time and closed-arm entries but showed no significant difference in open-arm time versus control.

Animal Model:	Wistar rats (male, 180-200 g)^[3]
Dosage:	5 μg/kg; 15 μg/kg; 50 μg/kg; 150 μg/kg
Administration:	i.p.; single dose, 12 min pre-stimulation
Result:	Increased flinching, vocalization, rearing, running, and fighting thresholds while reducing fight frequency at 5, 15, 50, and 150 μg/kg; the effects were most prominent at 150 μg/kg.

Animal Model:	CBA (weight 20-25 g)^[4]
Dosage:	0.5 mg/kg; 1.5 mg/kg; 5 mg/kg; 50 mg/kg; 150 mg/kg; 450 mg/kg
Administration:	i.p.; daily; 10 days
Result:	Increased hepatocyte mitotic index significantly at 1.5-450 mg/kg with no obvious dose dependence, dose-dependently reduced antibody-producing spleen cell number, and induced liver degeneration at 150 mg/kg and 450 mg/kg with more severe lesions at 450 mg/kg.

Animal Model:	Wistar (weight 180-220 g)^[4]
Dosage:	0.5 mg/kg; 1.5 mg/kg; 5 mg/kg; 50 mg/kg; 150 mg/kg; 450 mg/kg
Administration:	i.p.; daily; 10 days
Result:	Reduced the regional-versus-contralateral popliteal lymph node weight difference and nucleated cell count difference dose-dependently at 1.5-450 mg/kg, significantly inhibited delayed-type hypersensitivity, and induced obvious liver degeneration at 150 mg/kg and 450 mg/kg with more severe injury at 450 mg/kg.

Molecular Weight

400.43

Formula

C₁₆H₂₈N₆O₆

CAS No.

72957-37-0

Appearance

Solid

Color

White to off-white

SMILES

NCCCC[C@H](NC([C@@H](NC(CN)=O)CC1=CNC=N1)=O)C(O)=O.CC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (249.73 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4973 mL	12.4866 mL	24.9732 mL
5 mM	0.4995 mL	2.4973 mL	4.9946 mL
10 mM	0.2497 mL	1.2487 mL	2.4973 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.68%

Select Batch:

Data Sheet (277 KB) SDS (394 KB)

COA (247 KB) HNMR (281 KB) RP-HPLC (206 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Kukowska M, et al. In vitro studies of antimicrobial activity of Gly-His-Lys conjugates as potential and promising candidates for therapeutics in skin and tissue infections. Bioorg Med Chem Lett. 2015;25(3):542-546.

[2]. Bobyntsev II, et al. Anxiolytic Effects of Gly-His-Lys Peptide and Its Analogs. Bull Exp Biol Med. 2015;158(6).

[3]. Sever'yanova LА, et al. Effects of Tripeptide Gly-His-Lys in Pain-Induced Aggressive-Defensive Behavior in Rats. Bull Exp Biol Med. 2017 Dec;164(2):140-143.

[4]. Smakhtin MY, et al. Tripeptide Gly-His-Lys is a Hepatotropic Immunosuppressor. Bull Exp Biol Med. 2002;133(6):586.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.4973 mL	12.4866 mL	24.9732 mL	62.4329 mL
	5 mM	0.4995 mL	2.4973 mL	4.9946 mL	12.4866 mL
	10 mM	0.2497 mL	1.2487 mL	2.4973 mL	6.2433 mL
	15 mM	0.1665 mL	0.8324 mL	1.6649 mL	4.1622 mL
	20 mM	0.1249 mL	0.6243 mL	1.2487 mL	3.1216 mL
	25 mM	0.0999 mL	0.4995 mL	0.9989 mL	2.4973 mL
	30 mM	0.0832 mL	0.4162 mL	0.8324 mL	2.0811 mL
	40 mM	0.0624 mL	0.3122 mL	0.6243 mL	1.5608 mL
	50 mM	0.0499 mL	0.2497 mL	0.4995 mL	1.2487 mL
	60 mM	0.0416 mL	0.2081 mL	0.4162 mL	1.0405 mL
	80 mM	0.0312 mL	0.1561 mL	0.3122 mL	0.7804 mL
	100 mM	0.0250 mL	0.1249 mL	0.2497 mL	0.6243 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.